ANTIULCER ACTIVITY OF CONTROLLED RELEASE FORMULATION CONTAINING AQUEOUS EXTRACT OF ACACIA CATECHU WILLD ON RODENT MODELS
Objective: To study the Antiulcer activity of aqueous extract of acacia catechu willd on rodent models by controlled release formulation.
Methods: Microspheres were prepared by solvent evapoaration method and were evaluated for various parameters like SEM, stability and in vitro relese. And acacia catechu willd loaded microspheres were evaluated for in vivo study involving Gastric ulceration in rats was induced by Ethanol/HCl. Ulcer genic effect (Ulcer Index), pH and Total acidity, Histopathological studies.
Results: Results indicated that Acacia catechu willd microspheres contains some active constituents like flavanoid which are responsible for its anti ulcer activity. Also it was observed aqueous extract loaded microspheres showed maximum activity was found (200 mg/kg) with standard Cimetidine (100 mg/kg) also, the results revealed that aqueous extract microspheres at 200 mg/kg had reduced ulcer incidence significantly, when compared to the control as evident by decrease in ulcer score in the model.
Conclusion: Based on the result it can be concluded that microspheres loaded with aqueous extracts of Acacia catechu willd produced promising antiulcer activity and were safe.
2. Asolkar LV, Kakkar KK. Second supplement to glossary of indian medicinal plants with active principles. Part I. Publication and Information Directorate (CSIR), New Delhi; 1992. p. 201-3.
3. B Guizot, DJ Armstrong, PNC Elliot, JL Ford, C Rostron. Investigation of in vitro release characteristics of NSAID-loaded polylactic acid microspheres. J Microencapsulation 1996:13:701â€“8.
4. Bancroft JD, Cook BC. Manual of histological techniques. Churchill Livingstone, Edinburgh NY, Baron JH. Current views of the pathogenesis of peptic ulcer. Stand J Gastroenterol 1984. p. 7-20.
5. Bimla, Meera, Chander, Jagdish, Kalidhar SB. A review on the chemistry and bioactivity of acacia spp. J Med Aromatic Plants Sci 2005;3:51-90.
6. Friedman GD, Siegelaub AB, Seltzer C. Cigarettes, alcohol, coffee, and peptic ulcer. N Engl J Med 1974;290:469-73.
7. Sontag S, Graham DY, Belisto A. Cimetidine, cigarette smoking, and recurrence of duodenal ulcer. Med 1984;311:689-93.
8. Srivastava AK, Ridhurkar DN, Wadhwa S. Floating microspheres of cimetidine: formulation, characterization and in vitro evaluation. Acta Pharm 2005;55:277â€“85.
9. Kurata JH, Honda GD, Frankl H. Hospitalization and mortality rates for peptic ulcers: a comparison of a large health maintenance organization and the United States data. Gastroenterology 1982;83:1008-16.
10. Gattani YS, Bhagwat DA, Maske AP. Formulation and evaluation of intragastric floating drug delivery system of diltiazem hydrochloride. Asian J Pharm 2008;2:228-31.
11. Gledhill T, Howard OM, Buck M. Single nocturnal dose of H, receptor antagonist for the treatment of duodenal ulcer. Gut 1983;24:904-8.
12. Lakshmi T, Geetha RV, Roy Anitha. In vitro evaluation of antibacterial activity of acacia catechu willd heartwood extract. Int J Pharma Biosci 2012;2:12.
13. Evans WC, Trease Pharmacognosy. 16th ed. London: Saunders Elsevier; 2009. p. 232-4.
14. Kokate CK, Purohit AP, Gokhle S. B Pharmacognosy 13Th edition Pune Nirali Prakashan; 2007. p. 124.
15. Lakshmi T, Geetha RV, Roy Anita. Acacia catechu willdâ€“a pharmacological review. Int J Curr Res Rev 2011;3:1380-9.
16. Lam SK, Isenberg JI, Grossman MI. Rapid gastric emptying in duodenal ulcer patients. Dig Dis Sci 1982;27:598-604.
17. Malagelada JR, Lonstreth GF, Deering TB. Gastric secretion and emptying after ordinary meals in duodenal ulcer. Gastroenterology 1977;73:989-94.
18. McCoy RF, Greenberg GR, Baron JH. Duodenal pH in health and duodenal ulcer disease. Gut 1984;25:386-92.
19. Patel, Jayshree, Vipin, Shreyas. Antimicrobial screening and phytochemical analysis of the resin part of Acacia catechu pharmaceutical. Biol J 2009;47:34-7.
20. Mendel A. What has been happening to a duodenal ulcer? Gastroenterology; 1974. p. 1020-2.
21. Mizui T, Dotuchi M. Effect of polyamines on acid and an ethanol-induced gastric lesion in rats. Japan J Pharmacol 1983;33:939â€“45.
22. Naik GH, Priyadarsini KI, Satav JG, Banavalikar MM, Sohoni DP, Biyani MK, et al. the Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine. Phytochemistry 2003;63:97-104.
23. Patil SS, Bhide AA, Gorle AM. Antiulcer activity and Anti-inflammatory studies on acacia catechu. Indian Drugs 2010;47:52-3.
24. Rao PR, Seshadri TR. Epi-catechin from Acacia catechu. J Sci Indian Res 2008;2:59.
25. SK Bhattacharya. Hand Book of Medicinal Plant. Pointer publishers, Jaipur; 2001. p. 230.
26. Sethi RK, Guru P, Sahu SK, Das PK, Barik B. Design and development of drug loaded microsphere by utilizing emulsion solvent evaporation method. Int J Pharm Sci 2010;1:46-9.
27. Sharma P, Dayal R, Ayyer KS. Acylglucosterole from Acacia catechu. J Med Aromatic Plants Sci 1999;21:1002-5.
28. Wallis TE. Textbook of pharmacognosy, CBS Publishers and Distributors, New Delhi. 5th Edition; 2011. p. 133-7.
29. Pramod Kumar, Shahid H Ansari, Javed Ali. Herbal remedies for the treatment of periodontal disease-a patent review recent patents on drug delivery and formulation 2009;3:221-8.
30. Price WA, Grizzle JA, Postlewait RW. Results of operation for duodenal ulcer; 1970;131:233-44.