ANTIOXIDANT ACTIVITY AND TOTAL PHENOL AND FLAVONOIDS ANALYSIS OF SIBERIAN GINSENG ROOT
Objective: This study was examined to in vitro antioxidant activity and Total Phenol and Flavonoids content analysis of methanolic root extract of Eleutherococcus senticosus (Siberian ginseng).
Methods: 1,1-dephenyl-2-picryl-hydrazyl free radical scavenging and FRAP assay propose that antioxidant activity of methanol root extract because of reducing capacity of the antioxidant against oxidative effects of reactive oxygen species.
Results: Scavenging activity of Siberian ginseng root RC50 value was shown 713.42±11.55 µg/ml and reducing power 0.13±0.01 mmol/g was investigated. In addition, total phenol 12.6±1.13 mg GAE/g DW and total flavonoids 9.8±0.20 mg QE/g DW were recorded.
Conclusion: Although all tests were performed in vitro assay, these results recommend that Siberian ginseng root may be a good source of antioxidant ingrediant.
2. Huang L, Zhao H, Huang B, Zheng C, Peng W, Qin L. Acanthopanaxsenticosus: review of botany, chemistry and pharmacology. Pharmazie 2011;66:83-97.
3. Lee S, Shin KH. The chemistry of secondary products from Acanthopanax species and their pharmacological activities. Nat Prod Sci 2002;8:111-26.
4. Sun YL, Liu LD, Hong SK. Eleutherococcus senticosus as a crude medicine: a review of biological and pharmacological effects. J Med Plants Res 2011;5:5946-52.
5. Ahn J, Um MY, Lee H. Eleutheroside E, an active component of Eleutherococcussenticosus, ameliorates insulin resistance in type 2 diabetic db/db mice. Evid Based Complement Alternat Med 2013. Doi:10.1155/2013/934183
6. Lee D, Park J, Yoon J. Neuroprotective effects of Eleutherococcussenticosus bark on transient global cerebral ischemia in rats. J Ethnopharmacol 2012;139:6-11.
7. Hibasami H, Fujikawa T, Takeda H. Induction of apoptosis by Acanthopanaxsenticosus HARMS and its component, sesamin in human stomach cancer KATO III cells. Oncol Rep 2000;7:1213-6.
8. Soo KH, Young PS, Kyoung KE. Acanthopanaxsenticosus has a heme oxygenase-1 signaling-dependent effect on porphyromonasgingivalis lipopolysaccharide-stimulated macrophages. J Ethnopharmacol 2012;142:819-28.
9. Huang L, Zhao H, Huang B, Zheng C, Peng W, Qin L. Acanthopanaxsenticosus: review of botany, chemistry and pharmacology. Pharmazie 2011;66:83-97.
10. Ha ES, Hwang SH, Shin KS. Anti-metastatic activity of glycoprotein fractionated from Acanthopanaxsenticosus, the involvement of NK-cell and macrophage activation. Arch Pharm Res 2004;27:217-24.
11. Sofowora. Recent trends in research into african medicinal plants. J Ethnopharmacol 1993;38:209.
12. VL Singleton, JA Rossi. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Viticulture 1965;16:144–58.
13. Hyun TK, Kim MO, Lee H, Kim Y, Kim E, Kim JS. Evaluation of anti-oxidant and anti-cancer properties of Dendropanax morbifera leveille. Food Chem 2013;141:1947-55.
14. Lee YJ, Kim DB, Lee JS, Cho JH, Kim BK, Choi HS, et al. Antioxidant activity and anti-adipogenic effects of wild herbs mainly cultivated in Korea. Molecules 2013;18:12937-50.
This work is licensed under a Creative Commons Attribution 4.0 International License.