EFFICACY OF AQUEOUS AND METHANOLIC EXTRACTS OF PLANT BRASSICA RAPA LEAVES POTENTIAL ANTI-PSEUDOMONAL ACTIVITY
Keywords:Brassica rapa, Anti-pseudomanal activities, Agar well diffusion method, Gentamycin, Phytochemical analysis, Turnip green
Objective: Efficacy of Aqueous and Methanolic Extracts of Plant Brassica Rapa Leaves Potential Anti-Pseudomanal Activity
Methods: Phytochemical analysis of Brassica rapa belonging to the family was examined using Aqueous and Methanolic extracts. Methanolic and aqueous extracts of leaves part of the plant were investigated individually for Anti-pseudomanal activity by agar well diffusion method. The susceptibility of three gram-negative bacteria to the aqueous and Methanolic extracts of this plant was measured and the results compared with standard antibiotic Gentamycin. Both the extracts were tested against selected species of Pseudomonas aeruginosa MTCC2295 (G-ve), Pseudomonas fluorescens MTCC2421 (G-ve), and Pseudomonas alcaligenes MTCC7095 (G-ve), to find the inhibitory activities of microbes at the dose level of 50 and 100 µg/ml.
Results: The phytochemical analysis of Aqueous and Methanolic extracts confirmed the presence of Carbohydrates, Fat, proteins, Vitamins, Minerals, Electrolytes, sugar, soluble and insoluble fiber and amino acids. Methanolic extract of Brassica rapa showed considerably high Anti-pseudomonal activities against all bacteria than Aqueous extract.
Conclusion: The leaves of Brassica Rapa were found to be rich in sulphur and nitrogen-containing compounds. Brassicarapamethanolic extract has anti-pseudomonal effects against bacteria.
The current study's range of activity can provide a new plant source for safe anti-pseudomanal drugs.
Al-Snafi AE. The therapeutic importance of Cassia occidentalis-an overview. Indian J Pharm Sci Res. 2015;5(3):158-71.
Marbin M Ideen, Al-Snafi AE. The probable therapeutic effects of date palm pollens in treatment of male infertility. Tikrit J Pharm Sci. 2008;1(1):30-5.
Goel RK, Singh D, Lagunin A, Poroikov V. PASS-assisted exploration of 1028 new therapeutic potential of natural products. Med Chem Res. 2010;1029.
Nalamolu KR, Nammi S. Antidiabetic and renoprotective effects of the chloroform extract of terminalia chebula retz. seeds in streptozotocin-induced diabetic rats. BMC Complement Altern Med. 2006;6:17. doi: 10.1186/1472-6882-6-17, PMID 16677399.
Service RF. Antibiotics that resist resistance. Science. 1995;270(5237):724-7. doi: 10.1126/science.270.5237.724, PMID 7481757.
Walsh C. Where will new antibiotics come from? Nat Rev Microbiol. 2003;1(1):65-70. doi: 10.1038/nrmicro727, PMID 15040181.
Koehn FE, Carter GT. Tithe evolving role of natural products in drug discovery. Nat Rev Drug Discov. 2005;4(3):206-20. doi: 10.1038/nrd1657.
Ojala T, Remes S, Haansuu P, Vuorela H, Hiltunen R, Haahtela K, Vuorela P. Antimicrobial activity of some coumarin containing herbal plants growing in Finland. J Ethnopharmacol. 2000;73(1-2):299-305. doi: 10.1016/s0378-8741(00)00279-8, PMID 11025169.
Chopra RN, Layer SL, Chopra K. Glossary of Indian medicinal plants. New Delhi: Council of Scientific and Industrial Research; 1956. p. 75.
Sun R. Economic/Academic interest of Brassica rapa. In: Wang X, Kole C, editors, The Brasicarapa genome. Heidelberg, New York, Berlin, London: Springer; 2015. p. 1-16.
Kokate CK. Practical pharmacognosy. Vallabh Prakashan, Delhi, 4th Edition; 1994. p. 107-9.
Vishnoi NK. Advanced practical organic chemistry. 2007 ed. Delhi: Vikas Publishing House Pvt. Ltd, 2nd; 2007. p. 54-5.
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy, Nirali Prakashan, Pune. 36th ed. Vol. 255; 2006.
http://mtcc.imtech.res.in/aboutmtcc.php. [Last accessed on 05 Aug 2021].
Gunasekaran P. Laboratory Manual in Microbiology, New Age publisher. 1st ed. Vol. 77; 1995.
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