• TIRUVAIPATI SIRISHA Department of Microbiology, Government Medical College, Srikakulam Dist, Andhra Pradesh, India
  • SWAPNA SASAPU Department of Microbiology, Andhra Medical College, Visakhapatnam
  • PAYALA VIJAYALAKSHMI Department of Microbiology, GITAM Institute of Medical Sciences and Research, GITAM (Deemed to be University), Visakhapatnam 530045, Andhra Pradesh, India
  • PILLI HEMA PRAKASH KUMARI Department of Microbiology, GITAM Institute of Medical Sciences and Research, GITAM (Deemed to be University), Visakhapatnam 530045, Andhra Pradesh, India



MDR microorganisms, MPN, Water-borne diseases, Antibiotics


Objective: The aim of the present study was to determine the levels of groundwater contamination by isolating total coliforms, fecal coliforms, and bacterial pathogens.

Methods: A total of 32 water samples, comprising 5 samples from each GVMC urban area zone, Visakhapatnam were randomly collected from different areas. Evaluation of Total and fecal coliform counts was done by using multiple-tube fermentation technique and were reported as the most probable number (MPN) of organisms present per 100 ml of sample.

Results: The samples collected from the zones Yendada (zone I), Marripalem (zone IV), Chinna musidiwada (zone VI) showed the highest total and fecal coliform counts (MPN>180). Nearly 30 samples (94%) yielded bacterial isolates. Among the isolates Pseudomonas spp. showed highest susceptibility to cefepime and imipenem antibiotics. All the microorganisms isolated were multidrug resistant with a multidrug antibiotic resistant index more than 0.2.

Conclusion: These multidrug-resistant (MDR) organisms are considered as a major threat to the public health and should receive more attention.


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Akturk S. Adana determination of microbial quality in water from Adana‐Tufanbeyli road line. Adana: Cukurova Universitesi; 2009.

Baquero F, Martinez JL, Canton R. Antibiotics and antibiotic resistance in water environments. Curr Opin Biotechnol. 2008;19(3):260-5. doi: 10.1016/j.copbio.2008.05.006, PMID 18534838.

Korzeniewska E, Korzeniewska A, Harnisz M. Antibiotic-resistant Escherichia coli in hospital and municipal sewage and their emission to the environment. Ecotoxicol Environ Saf. 2013;91:96-102. doi: 10.1016/j.ecoenv.2013.01.014, PMID 23433837.

WHO. Water supply, sanitation and hygiene development. In: Water sanitation and Health; 2015. Available: hygiene/en/.2017.

Edqvist L, Pedersen K. Antimicrobials as growth promoters: resistance to common sense. In: MacGarvin M, Stirling A, Keys J, Wynne B, Guedes Vaz S, editors. Late lessons from early warnings: the precautionary principle 1896-2000; Harremoes P, Gee D. Copenhagen, Denmark: European Environmental Agency; 2001. p. 93-100.

Brown MG, Balkwill DL. Antibiotic resistance in bacteria isolated from the deep terrestrial subsurface. Microb Ecol. 2009;57(3):484-93. doi: 10.1007/s00248-008-9431-6, PMID 18677528.

Ellis H, Schoenberger E. On the identification of associations between five world health organization water, sanitation and hygiene phenotypes and six predictors in low and middle-income countries. PLOS ONE. 2017;12(1):e0170451. doi: 10.1371/journal.pone.0170451, PMID 28125625.

Bush K, Courvalin P, Dantas G, Davies J, Eisenstein B, Huovinen P. Tackling antibiotic resistance. Nat Rev Microbiol. 2011;9(12):894-6. doi: 10.1038/nrmicro2693, PMID 22048738.

Port JA, Cullen AC, Wallace JC, Smith MN, Faustman EM. Metagenomic frameworks for monitoring antibiotic resistance in aquatic environments. Environ Health Perspect. 2014;122(3):222-8. doi: 10.1289/ehp.1307009, PMID 24334622.

Munir M, Wong K, Xagoraraki I. Release of antibiotic-resistant bacteria and genes in the effluent and biosolids of five wastewater utilities in Michigan. Water Res. 2011;45(2):681-93. doi: 10.1016/j.watres.2010.08.033, PMID 20850863.

Samie A, Makonto TE, Odiyo J, Ouaboi-Egbenni PO, Mojapelo P, Bessong PO. Microbial quality, diversity and antibiotic susceptibility profiles of bacterial isolates from borehole water used by schools in Greater Giyani Municipality, Mopani District, South Africa. Afr J Microbiol Res. 2011;5(3):198-210.

Mwajuma JJ. Physico-Chemical and bacteriological quality of water, and antimicrobial susceptibility of pathogenic isolates from selected water sources in Samburu South,”a thesis submitted in partial fulfillment of the requirements for the award of the degree of master of science (Microbiology) in the school of pure and applied sciences, department of plant and microbial sciences, Kenyatta University; 2010. p. 8-90.

Marathe NP, Pal C, Gaikwad SS, Jonsson V, Kristiansson E, Larsson DGJ. Untreated urban waste contaminates Indian river sediments with resistance genes to last-resort antibiotics. Water Res. 2017;124:388-97. doi: 10.1016/j.watres.2017.07.060, PMID 28780361.

Mezrioui N, Baleux B. Resistance patterns of E. coli strains isolated from domestic sewage before and after treatment in both aerobic lagoon and activated sludge. Water Res. 1994;28(11):2399-406. doi: 10.1016/0043-1354(94)90056-6.

Feachem RG. Interventions for the control of diarrhoeal diseases among young children: promotion of personal and domestic hygiene. Bull World Health Organ. 1984;62(3):467-76. PMID 6331908.

Mari L, Casagrandi R, Rinaldo A, Gatto M. Epidemicity thresholds for water-borne and water-related diseases. J Theor Biol. 2018;447:126-38. doi: 10.1016/j.jtbi.2018.03.024, PMID 29588168.

Center for Disease Control (CDC). Antibiotic resistance questions and answers [Are antibacterial-containing products (soaps, household cleaners, etc.) better for preventing the spread of infection? Does their use add to the problem of resistance?]". Atlanta: Centers for Disease Control and Prevention; 2009.

Collee AG, Fraser BP, Marmion, Simmons. A editors. Mackie and McCartney practical medical microbiology. 14th ed JG, editor; 2000.

World Health Organization. Diarrhoeal disease fact sheet; 2016. Available from; [Last accessed on 23 Feb 2023]

Sartory DP, Spies K, Lange B, Schneider S, Langer B. Evaluation of a most probable number method for the enumeration of Legionella pneumophila from potable and related water samples. Lett Appl Microbiol. 2017;64(4):271-5. doi: 10.1111/lam.12719, PMID 28117485.

Krupanidhi KVJR. Mechanism of groundwater pollution in village wells. J Geol Soc India. 1984;25(5):301-2.

Walsh C. Antibiotics: actions, origins, resistance. Washington, DC: ASM Press; 2003.

Bessong PO, Odiyo JO, Musekene JN, Tessema A. Spatial distribution of diarrhoea and microbial quality of domestic water during an outbreak of diarrhoea in the Tshikuwi community in Venda, South Africa. J Health Popul Nutr. 2009;27(5):652-9. doi: 10.3329/jhpn.v27i5.3642, PMID 19902801.

Sandhu R, Dahiya S, Sayal P. Evaluation of multiple antibiotic resistance (MAR) index and doxycycline susceptibility of Acinetobacter species among inpatients. Indian J Microbiol Res. 2016;3(3):299-304. doi: 10.5958/2394-5478.2016.00064.9.



How to Cite

SIRISHA, T., S. SASAPU, P. VIJAYALAKSHMI, and P. H. P. KUMARI. “ISOLATION OF MULTI DRUG RESISTANT BACTERIA FROM BORE WATER SAMPLES IN AND AROUND VISAKHAPATNAM”. International Journal of Current Pharmaceutical Research, vol. 15, no. 2, Mar. 2023, pp. 26-32, doi:10.22159/ijcpr.2023v15i2.2083.



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