Quantitative evaluation of pollen protein from Helianthus annuus for honey bee nutrition


Amit Gupta

Abstract


Objective- The objective of our study is to estimate the protein content from pollen (flowers) of Helianthus annuus and also determined in bee pollen (collected from honey bees).

 

Methods: Nanodrop method is the most suitable method for protein estimation and also quantified the protein of pollen and bee pollen in KDa using SDS PAGE. In addition, proteins extracted from both the samples and exposed to hemolymph containing haemocytes pertaining to determine forward and side scatter using flow cytometry.

 

Results: The data showed that pollen contained higher amount of protein content as compared to bee pollen. While SDS-PAGE analysis represents bands of various protein size from 14 KDa to 67 KDa (pollen, 25KDa and 34KDa; bee pollen, 25KDa). In an effort to determine the effect of proteins extracted from pollen and bee pollen directly exposed to haemolymph after feeding with these proteins diets and determined its shape and size (forward scatter) and granularity (side scatter) of the haemocytes. The data indicates that proteins from bee pollen showed enhancement in forward and side scatter as compared to pollen.

 

Conclusion: Overall, the data suggests that bee pollen could be a potent candidate for nutrition to honey bees.

 

Key words: Helianthus annuus, pollen; protein, haemocytes, haemolymph, flow cytometry.


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About this article

Title

Quantitative evaluation of pollen protein from Helianthus annuus for honey bee nutrition

Date

01-01-2017

Additional Links

Manuscript Submission

Journal

Innovare Journal of Life Sciences
Vol 5 Issue 1 2017 (Jan-Feb) Page: 4-6

Online ISSN

2321-550X

Statistics

108 Views | 173 Downloads

Authors & Affiliations

Amit Gupta
India


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