• Charumathy M. Departmentof Biochemistry, Kongunadu Arts and Science College, Coimbatore 641029, Tamil Nadu, India
  • Sudha G.
  • Packialakshmi B.


Objectives: Hericium is a genus of mushrooms in the Hericiaceae family. Hericium erinaceus has been used for the treatment of various diseases for over 2000 y in China. The study investigated the bioactive components and antioxidant properties of the hot water extract from the fruiting body of H. erinaceus.

Methods: The hot water extract of H. erinaceus was estimated for antioxidant potency using different analysis, such as 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay, ferric reducing antioxidant power (FRAP) assay, cupric ions reducing antioxidant capacity (CUPRAC) assay, metal ion chelation, erythrocyte hemolysis inhibition assay and compared with the reference standards. Total phenolic, flavonoid contents, and total antioxidant capacity were also determined using spectrophotometric methods.

Results: H. erinaceus showed concentration dependent activity in all the assays with following EC50 values. DPPH (EC50= 2.13 mg/ml), FRAP (EC50= 7.57 mg/ml), CUPRAC (EC50= 0.97 mg/ml), ferrous ion chelation (EC50= 2.31 mg/ml) and erythrocytes hemolysis inhibition (EC50= 4.88 mg/ml). The total phenolic and flavonoid content in H. erinaceus was found to be 8.77 mg GAE/g and 5.62 mg RE/g respectively.

Conclusion: It was concluded that the hot water extract of the H. erinaceus exhibit potent free radical scavenging activity and can be used as natural antioxidants owing to their significant antioxidant activity.

Keywords: Antioxidant, Scavenging activity, Free radicals, Reducing power, Hericium erinaceus pers, Total flavonoids, Total phenolics


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1. Yazdanparast R, Ardestani A. In vitro antioxidant and free radical scavenging activity of Cyperus rotundus. J Med Food 2007;10:667-74.
2. Heleno SA, Barros L, Sousa MJ, Martins A, Ferreira ICFR. Tocopherols composition of Portuguese wild mushrooms with antioxidant capacity. Food Chem 2010;119:1443-50.
3. Reis FS, Barros L, Martins A, Ferreira ICFR. Chemical composition and nutritional value of the most widely appreciated cultivated mushrooms: an interspecies comparative study. Food Chem Toxicol 2012;50:191-7.
4. Tseng YH, Yang JH, Mau JL. Antioxidant properties of polysaccharides from Ganoderma tsugae. Food Chem 2008;107:732-8.
5. Yang BK, Park JB, Song CH. Hypolipidemic effect of an Exo-biopolymer produced from a submerged mycelia culture of Hericium erinaceus. Biosci Biotechnol Biochem 2003;67:1292-8.
6. Mizuno T, Wasa T, Ito H, Suzuki C, Ukai N. Antitumor active polysaccharides isolated from the fruiting body of Hericium erinaceum, an edible and medicinal mushroom called yamabushitake or houtou. Biosci Biotechnol Biochem 1992;56:347-8.
7. Okamoto K, Sakai T, Shimada A, Shirai R, Sakamoto H, Yoshida S, et al. Anti microbial chlorinated orcinol derivatives from mycelia of Hericium erinaceum. Phytochemistry 1993;34:1445-6.
8. Abdulla MA, Noor S, Wong KH, Ali HM. Effect of culinary-medicinal lion's mane mushroom, Hericium erinaceus (Bull. Fr.) Pers. (Aphyllophoromy-cetideae), on ethanol-induced gastric ulcers in rats. Int J Med Mushrooms 2008;10:325-30.
9. Zhang Z, Lv G, Pan H, Pandey A, He W, Fan L. Antioxidant and hepato protective potential of endo-polysaccharides from Hericium erinaceus grown on tofu whey. Int J Biol Macromol 2012;51:1140-6.
10. Wang JC, Hu SH, Wang JT, Chen KS, Chia YC. Hypoglycemic effect of an extract of Hericium erinaceus. J Sci Food Agric 2005;85:641-6.
11. Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the auto-oxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem 1992;40:945-8.
12. Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “Antioxidant power” The FRAP assay. Anal Biochem 1996;239:70-6.
13. Apak R, Guclu K, Ozyurek M, Karademir SE, Ercaq E. The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. Int J Food Sci Nutr 2006;57(5-6):292-304.
14. Balaydin HT, Gulcin I, Menzek, A, Goksu S, Sahin E. Synthesis and antioxidant properties of diphenylmethane derivative bromophenols including a natural product. J Enzyme Inhib Med Chem 2010;25:685-95.
15. Dinis TCP, Madeira VMC, Almeida LM. The action of phenolic derivatives (acetaminophen, salicylate, and 5‐amino salicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 1994;315:161‐9.
16. Barros L, Baptista P, Ferreira ICFR. Effect of Lactarius piperatus fruiting body maturity stage on antioxidant activity measured by several biochemical assays. Food Chem Toxicol 2007;45:1731-7.
17. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phospho- molybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337-41.
18. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965;16:144-58.
19. Jia Z, Tang M, Wu J. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999;64:555-9.
20. Banerjee A, Dasgupta N, De B. In vitro study of antioxidant activity of Syzygium cumini fruit. Food Chem 2005;90:727-33.
21. Naik GH, Priyadarsini KI, Satav JG, Banavalikar MM, Sohoni DP, Biyani MK, et al. the Comparative antioxidant activity of individual herbal components used in ayurvedic medicine. Phytochemistry 2003;63:97-104.
22. Ferreira ICFR, Baptista P, Vilas-Boas M, Barros L. Free-radical scavenging capacity and reducing power of edible wild mushrooms from the northeast portugal: individual cap and stipe activity. Food Chem 2007;100:1511-6.
23. Keleş A, Koca I, Gençcelep H. Antioxidant properties of wild edible mushrooms. J Food Process Technol 2011;2:1-6.
24. Mohamed AA, Ali SI, El-Baz FK. Antioxidant and antibacterial activities of crude extracts and essential oils of Syzygium cumini leaves. PLoS One 2013;8:1-7.
25. Shwetha VK, Sudha GM. In vitro free radical scavenging activity of aqueous extract from the mycelia of Volvariella volvacea (Bulliard Ex Fries) Singer. Int J Curr Pharm Res 2012;4:94-100.
26. Apak R, Guclu K, Ozyurek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agric Food Chem 2004;52:7970-81.
27. Gulcin I. Measurement of the antioxidant ability of melatonin and serotonin by the DMPD and CUPRAC methods as trolox equivalent. J Enzyme Inhib Med Chem 2008;23:871-6.
28. Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005;53:1841-56.
29. Abdullah N, Ismail SM, Aminudin N, Shuib AS, Lau BF. Evaluation of selected culinary-medicinal mushrooms for antioxidant and ACE inhibitory activities. J Evidence-Based Complementary Altern Med 2012;1-12. [Article in Press]
30. Chen H, Ju Y, Li J, Yu M. Antioxidant activities of polysaccharides from Lentinus edodes and their significance for disease prevention. Int J Biol Macromol 2012;50:214-8.
31. Vamanu E, Nita S. Antioxidant capacity and the correlation with major phenolic compounds, anthocyanin, and tocopherol content in various extracts from the wild edible Boletus edulis mushroom. Biomed Res Int 2013;1-11. [Article in Press]
32. Zou CG, Agar NS, Jones GL. Oxidative insult to human red blood cells induced by free radical initiator AAPH and its inhibition by a commercial antioxidant mixture. Life Sci 2001;69:75-86.
33. Costa RM, Magalhaes AS, Pereira JA, Andrade PB, Valentao P, Carvalho M, et al. Evaluation of free radical scavenging and antihemolytic activities of quince (Cydonia oblonga) leaf: a comparative study with green tea (Camellia sinensis). Food Chem Toxicol 2009;47:860-5.
34. Carvalho M, Ferreira PJ, Mendes VS, Silva R, Pereira JA, Jeronimo C, et al. Human cancer cell antiproliferative and antioxidant activities of Juglans regia L. Food Chem Toxicol 2010;48:441-7.
35. Packialakshmi B, Sudha G, Charumathy M. Total phenol, flavonoid and antioxidant properties of Auricularia Auricula-judae. Int J Pharm Sci 2015;7:1-5.
36. Stefano P, Crispian S. Polyphenols, oral health and disease: a review. J Dent 2009;37:413-23.
37. Agati G, Azzarello E, Pollastri S, Tattini M. Flavonoids as antioxidants in plants: location and functional significance. Plant Sci 2012;196:67-76.
38. Kosanic M, Rankovic B, Dasic M. Mushrooms as possible antioxidant and antimicrobial agents. Iran J Pharm Res 2012;11:1095-102.
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How to Cite
M., C., S. G., and P. B. “ANTIOXIDANT ACTIVITY AND BIOACTIVE CONSTITUENTS IN THE FRUITING BODIES OF HERICUM ERINACEUS PERS, AN EDIBLE MUSHROOM”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 3, Mar. 2016, pp. 152-6,
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