PHYTOCHEMICAL SCREENING AND HPTLC FINGERPRINTING ANALYSIS OF ETHANOLIC EXTRACT OF ERYTHRINA VARIEGATA L. FLOWERS
Objective: Medicinal plants possess phytochemicals that accumulate in various parts of plant like leaves, flowers, vegetables and roots that exert defense mechanism and protect us from various diseases. The present study was carried out to investigate the secondary metabolites and develop HPTLC fingerprinting of Erythrina variegata L.
Methods: The Phytochemical screening was analyzed for various extracts by using standard protocols and HPTLC analysis was carried out for the identification of alkaloids, flavonoids, glycosides, saponin and steroids in the ethanolic extract of Erythrina variegata L. flower.
Results: The phytochemicals screening confirms the presence of phyto-constituents in various plant extracts of Erythrina variegata L. The study revealed the presence of alkaloids, flavonoids, glycosides, saponin and steroids in the ethanolic extract of Erythrina variegata L. flower.
Conclusion: Based on the study it is concluded that Erythrina variegata L. flower possess phytochemicals like alkaloids, flavonoids, glycosides, saponin and steroids.
Keywords: Erythrina variegata L., HPTLC, Medicinal plants, Phytoconstituents, Secondary metabolites
2. Nile SH, Park SW. HPTLC densitometry method for simultaneous determination of flavonoids in selected medicinal plants. Front Life Sci 2014;8:1-7.
3. Zibbu G, Batra A. A review on chemistry and pharmacological activity of Nerium oleander L. J Chem Pharm Res 2010;2:351-8.
4. Sen S, Chakraborty R, Sridhar C, Reddy YSR, De B. Free radicals, antioxidants, diseases, and phytomedicines: current status and future prospect. Int J Pharm Sci Rev Res 2010;3:91-100.
5. Wadood A, Ghufran M, Jamal SB, Naeem M, Khan A, Ghaffar R, et al. Phytochemical analysis of medicinal plants occurring in the local area of mardan. Biochem Anal Chem 2013;2:1-4.
6. Alqasoumi SI, Alam P, Anwer MK, Abdel-Kader MS. Qualitative and quantitative analysis of khellin in Ammi visnaga friuts and pharmaceutical preparations using HPTLC and HPLC. J Liq Chromatogr Relat Technol 2014;37:61â€“72.
7. Gallo FR, Multari G, Federici E, Palazzino G, Giambenedetti M, Petitto V, et al. Chemical fingerprinting of Equisetum arvense L. using HPTLC densitometry and HPLC. Nat Prod Res 2011;25:1261â€“70.
8. Gallo FR, Multari G, Federici E, Palazzino G, Nicoletti M, Petitto V. The modern analytical determination of botanicals and similar novel natural products by the HPTLC fingerprint approach. In: Atta-ur-Rahman, editor. Studies in natural products chemistry. Bioact Nat Prod 2012;37:217â€“58.
9. Sheridan H, Krenn L, Jiang R, Sutherland I, Igntova S, Marmann A, et al. The potential of metabolic fingerprinting as a tool for the modernization of TCM preparations. J Ethnopharmacol 2012;140:482â€“91.
10. Uddin MMN, Emran TB, Mahib MMR, Dash R. Molecular docking and analgesic studies of Erythrina variegataâ€™s derived phytochemicals with COX enzymes. Bioinformatics 2014;10:630-6.
11. Mangathayaru K, Sarah K, Balakrishna K. Estrogenic effect of Erythrina variegata L. in the prepubertal female rat. Indian J Nat Prod Resour 2014;5:223-7.
12. Javed SB, Anis M. Cobalt induced augmentation of in vitro morphogenic potential in Erythrina variegata L.: a multipurpose tree legume. Plant Cell Tissue Organ Cult 2015;120:463-74.
13. Trease GE, Evans WC. Pharmacognosy. 11th edition. London: Bailliere Tindall Limited Publishers; 1978. p. 60-75.
14. Harborne JB. Phytochemical methods: a guide to modern techniques of plant analysis. 2nd edition. London: Chapman and Hall; 1984. p. 288.
15. Gomathi D, Ravikumar G, Kalaiselvi M, Vidya B, Uma C. HPTLC fingerprinting analysis of Evolvulus alsinoides (L.) L. J Acute Med 2012;2:77-82.
16. Rai MV, Pai VR, Kedilaya HP, Hegde S. Preliminary phytochemical screening of members of the lamiaceae family: Leucas linifolia, Coleus aromaticus and Pogestemon patchouli. Int J Pharm Sci Rev Res 2013;21:131-7.
17. Nagore DH, Patil PS, Kuber VV. Comparison between high-performance liquid chromatography and high-performance thin layer chromatography determination of diosgenin from fenugreek seed. Int J Green Pharm 2012;6:315-20.
18. Wang J, Peng Q, Li G. New compounds of natural resources in 2008. Afr J Biotechnol 2009;8:4299-307.
19. Edwin ON, Fidelia UI. Extraction and detection of the quaternary alkaloids of the stem bark of Alstonia boonei plant (family: Apocynaceae), by the sulfonephthalein dye-stuff ion-pair complexation at pH 5.58. J Pharm Biomed Sci 2012;15:1-4.
20. Kumar Y, Babujestadi D, Periyasamy L. Preliminary phytochemical screening, DNA protection, the antioxidant and antiproliferative effect of seed extracts of Bixa orellana L. Am J Phytomed Clin Ther 2014;2:1024-36.
21. Ugochukwu SC, Uche A, Ifeanyi O. Preliminary phytochemical screening of different solvent extracts of stem bark and roots of Dennetia tripetala G. Baker Asian J Plant Sci Res 2013;3:10-3.
22. Sharma V, Paliwal R. Isolation and characterization of saponins from Moringa oleifera (moringaeceae) pods. Int J Pharm Pharm Sci 2013;5:179-83.
23. Boukes GJ, Ventor MV, Oosthuizen V. Quantitative and qualitative analysis of sterols/sterolins and hypoxoside contents of three hypoxis (African potato) spp. Afr J Biotechnol 2008;7:1624-9.