ANTI-OXIDANT, ANTI-MICROBIAL AND GLUCOSE DIFFUSION INHIBITION ACTIVITIES OF THE AQUEOUS AND CHLOROFORM EXTRACT OF PHYLLANTHUS URINARIA
Objective: The aim of this research was to analyze the antioxidant, anti-microbial and in-vitro glucose diffusion inhibition of the phytochemicals extracted from Phyllanthus urinaria.
Methods: The anti-oxidant activity of the two extracts was done using DPPH method. The antimicrobial activity was performed using plate hole diffusion method. The in vitro anti-diabetic activity was determined using glucose diffusion inhibition technique.
Results: Both the aqueous and chloroform extract were shown to have declined in antioxidant activity with the increase in concentration. There was a considerable amount of zone of inhibition observed in antimicrobial activity when the assay was performed against E. coli. However, no such zone of inhibition was observed against Bacillus subtilis. The glucose diffusion inhibition was found to be maximum for chloroform extract.
Conclusion: The present study validates the effective use of P. urinaria against the microbial growth and also has anti-oxidant activity. The in vitro glucose diffusion inhibition proves the fact that it can act as a potent anti-diabetic agent.
Keywords: DPPH, Anti-oxidant, Anti-microbial, Glucose Diffusion Inhibition, Phytochemicals
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