IDENTIFICATION AND SCREENING OF EXTRACELLULAR VIRULENCE FACTORS OF AEROMONAS SPP. IN ENVIRONMENTAL WATER SAMPLES FROM TAMILNADU, SOUTH INDIA

  • Umapathy Indra
  • Palaniswamy Geetha
  • Balasubramanian Lakshman Kumar P. G. and Research Department of Biotechnology, Kongunadu Arts and Science College, G. N. Mills Post, Coimbatore 641029, Tamil Nadu

Abstract

Objective: Aeromonas species (spp.) are emerging pathogen that is present in drinking water supplies which are a major reason for public health concern. Factors contributing to virulence include toxins, haemolysins, adhesins and various hydrolytic enzymes. The present study aimed at investigating the incidence of Aeromonas spp. and its virulence factors from environmental water bodies in Coimbatore.

Methods: A total of 180 water samples were collected and screened for the presence of pathogenic Aeromonas spp. using starch ampicillin agar. The conventional method of identification might lead to misidentification of Aeromonas spp. thus molecular based identification using 16S rRNA gene was carried out followed by the identification of production of extracellular virulence factors like haemolysin, gelatinase, caseinase, lipase and DNase.

Results: Incidence of Aeromonas in water sample was found to be 55% (100 positive isolates) which was also confirmed with the molecular identification. The isolates showed 60% of haemolytic activity,, 34% of gelatinase activity, 77% of caseinase, 52% of lipase and 48% of DNase activity.

Conclusion: This study, thus, provides valuable information for the existence of pathogenic Aeromonas spp. in the environmental water bodies. Use of such water for domestic and commercial purpose might lead to the transfer of the pathogenic Aeromonas spp.

Keywords: Aeromonas spp, Haemolysis, Caseinase, DNase, Gelatinase and lipase.

Downloads

Download data is not yet available.

References

1. Pin C, Marin ML, Selgas D, Tormo MLGJ, Casas C. Differences in the production of several extracellular virulence factors in clinical and food Aeromonas sp strains. J Appl Bacteriol 1995;78:175-9.
2. Havelaar AH, Schets FM, Van Silfhout A, Jansen WH, Wieten G, van der Kooij D. Typing of Aeromonas strains from patients with diarrhoea and from drinking water. J Appl Bacteriol 1992;72:435-44.
3. Sen K, Rodgers M. Distribution of six virulence factors in Aeromonas species isolated from US drinking water utilities: A PCR identification. J Appl Microbiol 2004;97:1077-86.
4. Chopra AK, Xu XJ, Ribardo D, Gonzalez M, Kuhl K, Peterson JW, et al. The cytotoxic enterotoxin of Aeromonas hydrophila induces proinflammatory cytokine production and activates arachidonic acid metabolism in macrophages. Infect Immun 2000;68:2808-18.
5. Chacon MR, Figueras MJ, Castro-Escarpulli G, Soler L, Guarro J. Distribution of virulence genes in clinical and environmental isolates of Aeromonas spp. Antonie Van Leeuwenhoek 2003;84:269-78.
6. Bhowmik P, Bag PK, Hajra TK, Sarkar DPR, Ramamurthy T. Pathogenic potential of Aeromonas hydrophila isolated from surface waters in Kolkata, India. J Med Microbiol 2009; 58:1549-58.
7. John N, Hatha AAM. Distribution, extracellular virulence factors and drug resistance of motile Aeromonads in fresh water ornamental fishes and associated carriage water. Indian J Anaesth 2013;3:92-100.
8. Koksal F, Oguzkurt N, Samast M, Altas K. Prevalence and antimicrobial resistance patterns of Aeromonas strains isolated from drinking water samples in Istanbul, Turkey. Chemotherapy 2007;53:30-5.
9. Das A, Vinayasree CR, Santhosh CR, Sree Hari S. Surveillance of Aeromonas sobria and Aeromonas hydrophila from commercial food stuffs and environmental sources. J Exp Sci 2012;3:36-42.
10. Sechi LA, Deriu A, Falchi MP, Fadda G, Zanetti S. Distribution of virulence genes in Aeromonas spp. isolated from Sardinian waters and from patients with diarrhea. J Appl Microbiol 2002;92:221-7.
11. Yadav S, Verma DK, Pradhan PK, Dobriyal AK, Sood N. Phenotypic and genotypic identification of Aeromonas species from the aquatic environment. Int J Appl Sci 2014;5:3-20.
12. Suhet MI, Schocken-Iturrino RP, Amaral LA. Hemolytic activity and resistance to antimicrobials by Aeromonas species isolated from the Intensive rearing of Nile Tilapia (Oreochromis niloticus). ARS Veterinaria, Jaboticabal, SP; 2011;27:36-44.
13. Illanchezian S, Jayaraman S, Manoharan MS, Valsalam S. Virulence and cytotoxicity of seafood-borne Aeromonas hydrophila. Braz J Microbiol 2010;41:978-83.
14. Alzainy ZAA. The occurrence, hemolytic, cytotoxic activity and antibiotic susceptibility of Aeromonas hydrophila isolated from fish samples in baghdad. Iraqi J Veterinary Sci 2011;35:123-35.
15. Castro-Escarpulli G, Figueras MJ, Aguilera-Arreola G, Soler L, Fernandez-Rendon E, Aparicio GO, et al. Characterisation of Aeromonas spp. isolated from frozen fish intended for human consumption in Mexico. Int J Food Microbiol 2003;84:41-9.
16. Bagyalakshmi B, Sridhar D, Ponmurugan P, Smitha AJ, Arti K, Vidyashri T, et al. Characterization, hemolysis and multidrug resistance among Aeromonas spp. isolated from Bhavani River, Erode, South India. Recent Res Sci Technol 2009;1:14-9.
17. Erdem B, Kariptas E, Kaya T. Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish. Turk J Biol 2010;34:453-62.
18. Kozinska A. Dominant pathogenic species of mesophilic aeromonads isolated from diseased and healthy fish cultured in Poland. J Fish Dis 2007;30:293-301.
Statistics
406 Views | 421 Downloads
How to Cite
Indra, U., P. Geetha, and B. L. Kumar. “IDENTIFICATION AND SCREENING OF EXTRACELLULAR VIRULENCE FACTORS OF AEROMONAS SPP. IN ENVIRONMENTAL WATER SAMPLES FROM TAMILNADU, SOUTH INDIA”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 3, Mar. 2016, pp. 388-9, https://innovareacademics.in/journals/index.php/ijpps/article/view/10235.
Section
Short Communication(s)