STUDY OF ANTIBACTERIAL ACTIVITY OF RESINS OF BOSWELLIA SERRATA ROXB EX COLEBR., COMMIPHORA MUKUL (HOOKS EX-STOCKS) ENGL., GARDENIA RESINIFERA ROTH. AND SHOREA ROBUSTA GAERTN
Objective: To evaluate the antibacterial activity of the resins of Boswellia serrata, Commiphora mukul, Gardenia resinifera, Shorea robusta against Gram-positive bacteria namely Bacillus cereus and Staphylococcus aureus and Gram-negative bacteria namely Proteus vulgaris and Esherichia coli.
Methods: Resin samples (20%) were separately prepared in ethanol, DMSO, acetone and chloroform by overnight soaking 2 g of sample in 10 ml of solvent, filtered next day and were tested for the antibacterial activity by following standard method (well diffusion). The MIC was determined for the samples exhibiting+ve activity.
Results: The study revealed that resin of Shorea did not inhibit the growth of both Gram+ve and Gram-ve bacteria. The resin of Boswellia and Commiphora showed activity against B. cereus and the MIC was found to be 20 mg/ml and 200 mg/ml respectively. Resin of Gardenia exhibited activity against B. cereus (MICâ€“20 mg/ml and S. aureus (MICâ€“100 mg/ml).
Conclusion: Shorea did not have any antibacterial activity. The resins of B. serrata, C. mukul, and G. resinifera exhibited activity against Gram-positive bacteria; comparable to standard antibiotic amoxicillin, but they did not have the activity against Gram-negative bacteria. The maximum zone of inhibition (ZOI) was observed against B. cereus by Boswellia serrata resin sample prepared in ethanol.
Keywords: Resins, Boswellia serrata, Commiphora mukul, Gardenia resinifera, Shorea robusta, antibacterial activity, Gram positive, Gram negative
Murthy TK. Minor forest produces used in pharmaceutical and other industries. Hyderabad: Pharma Med Press; 2010.
Nadkarni KM. The Indian Materica Medic. Vol. I. 3rd ed. Ahmedabad: Popular Prakashan; 1976.
Joshi SG. Medicinal plants. New Delhi, Calcutta: Oxford and IBH publishing Co. Pvt. Ltd.; 2000.
National Institute of Science Communication, CSIR (IN). The Wealth of India-A Dictionary of Indian Raw materials and Industrial Products. Vol. IV. New Delhi: CSIR; 1976.
Sridhar PG, Harikiran L, Appa RA, Narsimha RN. Evaluation of anticancer activity of Dikamaliartane-A, A Cycloartane isolated from dikamali, a gum resin. Int J Pharm Pharm Sci 2012;4:501-4.
Hasson SS, Al-Balushi MS, Sallam TA, Idris MA, Habbal O, Al-Jabri AA. In vitro antibacterial activity of three medicinal plants-Boswellia (Luban) species. Asian Pac J Trop Biomed 2011;1: S178-S182.
Alam M, Khan H, Samiullah L, Siddque KM. A review on Phytochemical and pharmacological studies of Kundur (Boswellia serrate Roxb ex Colebr.)â€“A Unani drug. J Appl Pharm Sci 2012;2:148-56.
Abdallah EA, Khalid AS, Ibhrahim N. Antibacterial activity of oleo-gum resins of Commiphora molmol and Boswellia papyrifera against methicillin-resistant Staphlococcus aureus (MRSA). Sci Res Essay 2009;4:351-6.
Murthy KSR, Lakshmi N, Ramulu DR. Biological activity and phytochemical screening of the oleoresin of Shorea robusta Gaertn. f. Trop Subtrop Agroecosyst 2011;14:787-91.
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 46th ed. Pune: Nirali Prakashan; 2007.
Mahadlek J, Phachamud T, Wessapun C. Antimicrobial studies of Sonneratia caseolaris using different agar diffusion method. Res J Pharm Biol Chem Sci 2012;3:404-10.
Ramakrishnan G, Kothari R, Jayakar B, Venkata Rathnakumar T. In vitro antibacterial activity of different extracts of leaves of Coldenia procumbens. Int J PharmTech Res 2011;3:1000-4.
Rajendra CE, Harish Kumar DH, Yeshoda SV, Mahaboob Ali Nadaf, Hanumanthraju N. Comparative evaluation of antimicrobial activities of methanolic extract of Curcuma longa and Boswellia serrata. Int J Res Pharm Chem 2013;3:534-6.
Shareef Ali A. Evaluation of antibacterial activity of essential oils of Cinnamomum sp. And Boswellia sp. J Basrah Res (Sci) 2011;37:60-70.
Ishnava KB, Mahida YN, Mohan JSS. In vitro assessments of antibacterial potential of Commiphora wightii (Arn.) Bhandari. gum extract. J Pharmacogn Phytother 2010;2:91-6.