IN VITRO ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF RUTIN AND PIPERINE AND THEIR SYNERGISTIC EFFECT

Authors

  • Prapurnachandra Yadala
  • A. H. M. Viswanathswamy

Abstract

Objective: The objective of the present study was to evaluate the antioxidant and cytotoxic activities of Rutin and Piperine and their synergistic effect.

Methods: The Oxidative activity of Rutin, Piperine, and combination of both was confirmed by the level of Superoxide radical (SOD), Nitric oxide (NO), and Reducing power method (RO), Cytotoxic activity was also measured by using brine shrimp lethality bioassay.

Results: The present study showed that combination of Rutin and Piperine showed enhanced antioxidant activity in SOD and NO compared to individuals with IC50 values 27.18±0.59 µg/ml and 22.00±0.15 µg/ml respectively and also produced good reducing power activity. All samples showed a potent cytotoxic effect, combinational sample showed a more potent effect than the standard potassium dichromate with LC50 3.96 µg/ml.

Conclusion: Therefore, the present study demonstrated that the combination of Rutin and Piperine exhibits synergistic antioxidant and cytotoxic effect and the study thus provide more insight into the mechanism of the hepato protective action of the combinational sample for the management of hepatotoxicity.

Keywords: Rutin, Piperine, Antioxidant, Cytotoxic, SOD, NO, RO

 

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References

Ferenci P, Fried M, Labrecque D, Bruix J, Sherman M. Hepatocellular carcinoma (HCC): a global perspective. J Clin Gastroenterol 2010;44:239-45.

Arya SC, Ashraf SJ, Parande CM, Tobeiqi MS, Ageel AR. Hepatitis B and delta markers in primary hepatocellular carcinoma patients in the gizan area of Saudi Arabia. APMIS Suppl 1988;3:30-4.

Asmaa Ibrahim Gomaa, Shahid A Khan, Mireille B Toledano, Imam Waked, Simon D Taylor-Robinson. Hepatocellular carcinoma: epidemiology, risk factors, and pathogenesis. World J Gastroenterol 2008;14:4300–8.

Hideaki Tsukuma, Tomohiko Hiyama, Akira Oshima, Tomotaka Sobue, Isaburo Fujimoto. A case-control study of hepatocellular carcinoma in osaka, Japan. Int J Cancer 1990;45:231-6.

Wildi S, Pestalozzi BC, McCormack L, Clavien PA. Critical evaluation of the different staging systems for hepatocellular carcinoma. Br J Surg 2004;91:400-8.

Carnat AP, Carnat A, Fraisse D, Lamaison JL, Heitz A, Wylde R, et al. Violarvensin, a new flavone di-C-glycoside from viola arvensis. J Nat Prod 1998;61:272–4.

Goncalves AFK, Friedrich RB, Boligon AA, Piana M, Beck RCR, Athayde ML. Antioxidant capacity, total phenolic contents, and HPLC determination of rutin in Viola tricolor (L) flowers. Free Radicals Antioxid 2012;2:32–7.

Buszewski B, Kawka S, Suprynowicz Z, Wolski T. Simultaneous isolation of rutin and esculin from plant material and drugs using solid-phase extraction. J Pharm Biomed Anal 1993;11:211–5.

Tang DQ, Wei YQ, Gao YY, Yin XX. Protective effects of rutin on rat glomerular mesangial cells cultured in high glucose conditions. Phytother Res 2011;25:1640–7.

Wu CH, Lin MC, Wang HC, Yang MY, Jou MJ, Wang CJ. Rutin inhibits oleic acid induced lipid accumulation via reducing lipogenesis and oxidative stress in hepatocarcinoma cells. J Food Sci 2011;76:65–72.

Chen S, Gong J, Liu F, Mohammed U. Naturally occurring polyphenolic antioxidants modulate IgE-mediated mast cell activation. Immunology 2000;100:471–80.

Lee S, Suh S, Kim S. Protective effects of the green tea polyphenol (-)-epigallocatechin gallate against hippocampal neuronal damage after transient global ischemia in gerbils. Neurosci Lett 2000;287:191–4.

Novakovic A, Gojkovic-Bukarica L, Peric M, Nezic D, Djukanovic B, Markovic-Lipkovski J. The mechanism of endothelium-independent relaxation induced by the wine polyphenol resveratrol in the human internal mammary artery. J Pharmacol Sci 2006;101:85–90.

Singh YN. Kava an overview. J Ethanopharmacol 1992;37:18–25.

Singh YN, Blue Menthal M. Kava an overview. Herbal Gram 1997;39:34-55.

Virinder SP, Subash CJ, Kirpal SB, Rajani J. Phytochemistry of genus Piper. Phytochemistry 1997;46:597–603.

Amritpal Singh, Sanjiv Duggal. Piperine-review of advances in pharmacology. Int J Pharm Sci Nanotechnol 2009;2:615-20.

Meyer AS, Isaksen A. Application of enzymes as food antioxidants. Trends Food Sci Technol 1995;6:300–4.

Robak J, Gryglewski RJ. Flavonoids are scavengers of superoxide anions. Biochem Pharmacol 1988;37:837–41.

David SB. Endogenous nitric oxide synthesis: biological functions and pathophysiology. Free Radical Res 1999;31:577–96.

Ghafourifar P, Cadenas E. Mitochondrial nitric oxide synthase. Trends Pharmacol Sci 2005;26:190–5.

Marcocci L, Marguire JJ, Droy-lefaiz MT, Packer L. The nitric oxide scavenging properties of Ginkgo biloba extract. Biochem Biophys Res Commun 1994;201:748–55.

Oyaizu M. studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 1986;44:307-15.

Jayaprakash GK, Singh RP, Sakariah KK. Antioxidant activity of grape seed extracts on peroxidation models in vitro. J Agric Food Chem 2001;55:1018–22.

Mittal R, Gupta RL. In vitro antioxidant activity of Piperine. Methods find Exp Clin Pharmacol 2000;22:271-4.

Kester DR, IW Duedall, DN Connors, RM Pytkowicz. Preparation of artificial seawater. Limnol Oceanogr 1967;12:176-9.

Pietta PG. Flavonoids as antioxidants. J Nat Prod 2000;63:1035-42.

Wickens AP. Aging and the free radical theory. Respir Physiol 2001;128:379-91.

Yen G, Duh P. Scavenging effect of methanolic extracts of peanut hulls on free-radical and active oxygen species. J Agric Food Chem 1994;42:629-32.

Parejo I, Viladomat F, Bastida J, Rosas-Romero A, Flerlage N, Burillo J, et al. Comparison between the radical scavenging activity and antioxidant activity of six distilled and nondistilled Mediterranean herbs and aromatic plants. J Agric Food Chem 2002;50:6882-90.

Chung YC, Chang CT, Chao WW, Lin CF, Chou ST. Antioxidative activity and safety of the 50% ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1. J Agric Food Chem 2002;50:2454-8.

Illhmi Gulcin, Zubeyr Huyut, Mahfuz Elmastas, Hassan Y, Aboul-Enein. Radical scavenging and antioxidant activity of tannic acid. Arabian J Chem 2010;3:43–53.

Meyer BN, NR Ferrigni, JE Putnam, LB Jacobsen, DE Nichols, JL McLaughlin. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med 1982;45:31-4.

Lincoln RD, K Strupinski, JM Walker. The use of Artemia naupli (Brine shrimp larvae) to detect toxic compounds from microalgal cultures. Pharm Biol 1996;34:384-9.

Bergamini CM, S Gambelli, A Dondi, C Cervallati. Reative oxygen species and tissue damage. Curr Pharm Des 2004;10:1611-26.

Fridovich I. Oxygen toxicity: a radical explantation. J Exp Biol 1998;201:1203-9.

Hazra B, S Biswas, N Mandal. Antioxidant and free radical scavenging activity of Spondias pinnate. BMC Complementary Altern Med 2008;8:6882-30.

Rasool MK, EP Samba, K Lavanya, P Nitya. Therapeutic effect of Indian ayurvedic formulation triphala on acetaminophen-induced hepatotoxicity in mice. J Pharmacol Toxicol 2007;2:725-31.

Pimple BP, PV Kadam, NS Badgujar, AR Banfa, MJ Patil. Protective effect of Taminrundus indical Linn. against paracetamol induced hepatotoxicity in rats. Indian J Pharm Sci 2007;69:827-31.

Chuchawankul S, Khorana N, Poovorawan Y. Piperine inhibits cytokine production by human peripheral blood mononuclear cells. Genet Mol Res 2012;11:617–27.

Amrutpal Singh, Sanjiv Duggal. Piperine-review of advances in pharmacology. Int J Pharm Sci Nanotechnol 2009;2:615-20.

Rui Hai Liu. Potential synergy of phytochemicals in cancer prevention: mechanism of action. J Nutr 2004;134:3479-85.

Published

01-05-2016

How to Cite

Yadala, P., and A. H. M. Viswanathswamy. “IN VITRO ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF RUTIN AND PIPERINE AND THEIR SYNERGISTIC EFFECT”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 5, May 2016, pp. 78-82, https://journals.innovareacademics.in/index.php/ijpps/article/view/10426.

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