GALECTIN-3 IN REGULATION OF INFLAMMATORY RESPONSES MEDIATED BY LIPOPOLYSACCHARIDE IN MACROPHAGES AND ADIPOCYTES CULTURE SYSTEMS
Objective: The rationale of the present research work was to get insights of galectin-3 function in modulating inflammation in macrophages and adipocytes culture systems.
Methods: Recombinant galectin-3 was prepared, and anti-inflammatory effect of galectin-3 was studied in 3T3-L adipocytes and RAW264.7 macrophages stimulated with lipopolysaccharide followed by western blot analysis. Furthermore, we determined the galectin-3 effect on LPS-mediated ROS and NO generation in RAW macrophage cells by using DHE and mitochondrial membrane potential was measured by JC-1 respectively.
Results: Galectin-3 negatively regulates the exaggerated inflammatory response in the presence of lipopolysaccharide, by lowering cytokines in adipocytes and macrophages. Reduced oxidative stress was evident as the production of ROS and NO was diminished to a great extent by galectin-3 in lipopolysaccharide-treated macrophages. This was also confirmed by the ability of galectin-3 in the maintenance of mitochondrial membrane potential against lipopolysaccharide-induced massive membrane depolarization by galectin-3.
Conclusion: Based on the results obtained, it is rational to mention that galectin-3 exhibits significant anti-inflammatory and anti-oxidative effects in adipocyte and macrophage culture systems, when exposed to lipopolysaccharide.Keywords: Galectin-3, Adipocytes, Macrophages, LPS, Glycobiology, Toll-like receptor
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