ANTIFERTILITY ACTIVITY OF β-SITOSTEROL ISOLATED FROM BARLERIA PRIONITIS (L.) ROOTS IN MALE ALBINO RATS.
Keywords:
Libido, Fertility, Active component, NMR, Sperm, Testosterone, Contraceptive, Spermatogenesis, NilAbstract
Objective: Plant–derived male antifertility agents need to address the basic requirements of fertility suppression without affecting libido. This study was undertaken to evaluate the male antifertility potential of the β-sitosterol (BS) which is an active component isolated from the methanolic root extract of Barleria prionitis in the male albino rats.
Methods: β-sitosterol was extracted using silica gel column chromatography from the methanolic root extract of Barleria prionitis and characterized by IR and NMR spectral analysis. Extracted BS was further used to determine its antifertility activity. The rats were orally administered olive oil (Group-I, control), BS at the dose level of 5 (Group II), 15 (Group III) and 25 mg/kg body weight (Group IV) for 60 d. Body weight was measured weekly. At the end of the experimental duration, treated males were sacrificed and subjected to biochemical, hormonal and sperm analysis. Fertility was assessed by mating treated rats with normally cycling virgin females.
Results: Average weight of reproductive organs, serum levels of Testosterone, Follicular stimulating hormone (FSH) and Luteinizing hormone (LH), levels of Protein, Ascorbic acid, Glycogen, Fructose, sperm motility and sperm density was decreased significantly as compared to control group. While, Cholesterol level was increased significantly. Fertility percentage was also decreased in treated groups. Body weight remained unchanged.
Conclusion: The results show that β-sitosterol from the roots of Barleria prionitis causes suppression of spermatogenesis and fertility thereby suggesting the potential of β-sitosterol for the development of male contraceptive which has very limited options available.
Keywords: Libido, Fertility, Active component, NMR, Sperm, Testosterone, Contraceptive, SpermatogenesisÂ
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Thangakrishnakumari S, Sakhtidevi G, Muthukumaraswami S, Mohan VR. Antifertility activity of whole plant extracts of Sarcostemma secamone (L) bennet on male albino rats. Int Res J Pharm 2012;3:139-44.
Shajeela PS, Mohan VR, Jesudas LL, Soris PT. Antifertility activity of ethanol extract of Dioscorea esculenta (L.) Schott on male albino rats. Int J PharmTech Res 2011;3:946-54.
Deshgan MH, Daryani A, Robabeh D. Histological evidence of male potent reproductive sites by Iranian botanical Azadirachta indica (neem) seed extract. Int J Mol Med Adv Sci 2006;2:7-15.
Gupta RS, Kachhawa JB, Chaudhary R. Antifertility effects of methanolic pod extract of Albizzia lebbeck (L.) Benth in male rats. Asian J Androl 2004;6:155-9.
Sharma N, Kachhawa JBS, Tyagi S, Gupta RS, Sharma KK. In vitro evaluation of antibacterial activity of Pterocarpus marsupium Roxb. Int J Pharm Pharm Sci 2012;4:67-8.
Cragg GM, Newman DJ. Medicinals for the millennia. Ann N Y Acad Sci 2001;953:3-25.
Khare CP. Indian medicinal plants: An Illustrated Dictionary. 1st Ed. New York: Springer Sci; 2007. p. 82-3.
Shukla P, Singh A, Gawri S, Alexande A, Sonwane S. In vitro propagation of Barleria prionitis Linn and its antibacterial activity. Int J Pharma Professional Res 2011;2:198-200.
Jaiswal SK, Dubey MK, Das S, Verma AR, Vijaykumar M, Rao CV. Evaluation of flower of Barleria prionitis for anti–inflammatory and anti–nociceptive activity. Int J Pharma Bio Sci 2010;1:1–10.
Verma PK, Sharma A, Joshi SC, Gupta RS, Dixit VP. Effect of Isolated fractions of Barleria prionitis root methanolic extract on reproductive function of male rats: preliminary study. Fitoterapia 2005;76:428–32.
Chavan CB, Shinde UV, Hogade M, Bhinge S. Screening of in vitro antibacterial assay of Barleria prionitis Lin. J Herb Med Toxicol 2010;4:197-200.
Dheer R, Bhatnagar P. A study of the antidiabetic activity of Barleria prionitis Linn. Indian J Pharmacol 2010;42:70–3.
Singh B, Bani S, Gupta DK, Chandan BK, Kaul A. Anti–inflammatory activity of ‘TAF’ an active fraction from the Barleria prionitis Linn. J Ethnopharmacol 2003;85:187–93.
Singh B, Chandan BK, Prabhakar A, Taneja SC, Singh J, Qazi GN. Chemistry and hepatoprotective activity of an active fraction from Barleria prionitis Linn. in experimental animals. Phytother Res 2005;19:391–404.
Amoo SO, Ndhlala AR, Finnie JF, Van Staden J. Anti–fungal, acetylcholinesterase inhibition, antioxidant and phytochemical properties of three Barleria species. S Afr J Bot 2011;77:435–45.
Suri JL, Banerjee SK, Taneja SC, Chandra S, Anand AS, Prabhakar A, et al. U S Pat Appl Publ; 2003.
Gupta RS, Kumar P, Dixit VP, Dobhal MP. Antifertility studies of the root extract of the Barleria prionitis Linn in male albino rats with special reference to testicular cell population dynamics. J Ethnopharmacol 2000;70:111-7.
Prieto JM, Recio MC, Rosa M, Giner. Anti-inflammatory activity of β-sitosterol in a model of oxazolone induced contact-delayed-type hypersensitivity. Bol Latinoam Caribe Plant Med Aromat 2006;5:57-62.
Paniagua-perez R, Madrigal-bujaidar E, Reyes-cadena S, Molina-Jasso D, Gallaga JP, Silva-Miranda A. Genotoxic and cytotoxic studies of beta-sitosterol and pteropodine in mouse. J Biomed Biotechnol 2005;3:242-7.
Gupta R, Sharma AK, Dobhal MP, Sharma MC, Gupta RS. Antidiabetic and antioxidant potential of b-sitosterol in streptozotocin-induced experimental hyperglycemia. J Diabetes 2011;3:29-37.
Jamaluddin F, Mohamed S, Lajis MN. Hypoglycaemic effect of Parma speciosa seeds due to the synergistic action of β-sitosterol and stigmasterol. Food Chem 1994;49:339-45.
Radika MK, Viswanathan P, Anuradha CV. Nitric oxide mediates the insulin sensitizing effects of β-sitosterol in high fat diet-fed rats. Nitric Oxide 2013;32:43-53.
Kiprono PC, Kaberia F, Keriko JM, Karanja JN. The in vitro anti-fungal and anti-bacterial activities of beta-sitosterol from Senecio lyratus (Asteraceae). Z Naturforsch C 2000;55:485-8.
Sen A, Dhavan P, Khukla KK, Singh S, Tejovathi G. Analysis of IR, NMR and antimicrobial activity of β-sitosterol isolated from Momordica charantia. Sci Secure J Biotechnol 2012;1:9-13.
Villasenor IM, Angelada J, Canlas AP, Echegoyen D. Bioactivity studies on beta-sitosterol and its glucoside. Phytother Res 2002;16:417-21.
Jourdain C, Tenca G, Deguercy A, Troplin P, Poelman D. In vitro effects of polyphenols from cocoa and beta-sitosterol on the growth of human prostate cancer and normal cells. Eur J Cancer Prev 2006;15:353-61.
Holtz RL, Fink CS, Awad AB. Beta-sitosterol activates the sphingomyelin cycle and induces apoptosis in LNCaP human prostate cancer cells. Nutr Cancer 1998;32:8-12.
Harborne JB. Phytochemical methods: a guide to modern techniques of plant analysis. 3rd Ed. London: Chapman and Hall; 1998. p. 129-38.
Walum E. Acute oral toxicity. Environ Health Perspect 1998;106:497-503.
Nostro F, Geimano MP, Angela VP, Marino A, Cannatell MA. Extraction methods and bioautography for evolution of medicinal plant antimicrobial activity. Left Appl Microbiol 2000;30:379-84.
Britto AJD, Gracelin DHS, Sebastian SR. Antibacterial activity of a few medicinal plants against Xanthomonas campestris and Aeromonas hydrophila. J Biopestic 2011;4:57-60.
Asuquo OR, Ekanem TB, Udoh PB, Eluwa MA. Histomorphological study of the anti-fertility effect of Spondias mombin L. in adult male rats. IOSR-JBPS 2012;3:29-34.
Kamboj A, Saluja AK. Isolation of stigmasterol and β ¬sitosterol from petroleum ether extract of aerial parts of Ageratum conyzoides (asteraceae). Int J Pharm Pharm Sci 2011;3:94-6.
Chaturvedula VSP, Prakash I. Isolation of stigmasterol and β-sitosterol from the dichloromethane extract of Rubus suavissimus. Int J Chem Pharm Res 2012;1:239-42.
Akhtar P, Ali M, Sharma MP, Farooqi H, Khan HN. Phytochemical investigation of fruits of corylus colurna linn. J Phytol 2010;2:89-100.
Bulama JS, Dangoggo SM, Mathias SN. Isolation and characterization of beta-sitosterol from ethyl acetate extract of root bark of Terminalia glaucescens. Int J Sci Res Publications 2015;5:1-3.
Sarin R, Bansal N. Phytosterol from in vivo and in vitro cultures of two medicinal plants viz. Adhatoda vasica and Ageratum conizoidus. Int J Res Ayurveda Pharm 2011;2:927-30.
Kim HC, Chung HY, Lee SY, Kim YJ, Baek NI, Kim SH, et al. Production of β-sitoterol by cell suspension culture of Chrysanthemum coronarium L. J Korean Soc Appl Biol Chem 2005;48:425-30.
Vijaykumar B, Sangamma I, Sharanabasappa A, Saraswati BP. Antifertiltiy activity of various extracts of Crotalaria juncea Linn. Seeds in male mice. Philipp J Sci 2003;132:39-46.
Snedchor CW. Statistical methods (Iowa State College Press, Ames, Iowa); 1946. p. 22.
Vijaykumar BM, Saraswati BP. Spermatogenic index and hormonal profile in the rats received chromatographic fractions of ethanol extract of Crotalaria juncea Linn. Seeds, Orient. Pharm Exp Med 2006;6:286-95.
Londonkar RL, Reddy PS, Patil SR, Patil SB. Nicotine induced inhibition of the activities of accessory reproductive ducts in male rats. J Ethnopharmacol 1998;60:215-21.
Zeherea MN, Reddy SP, Ravindra, Saraswati Patil B. Antispermatogenic and androgenic activities of Momordica Charantia (Karela) in albino rats. J Ethnopharmacol 1998;61:9-16.
Choudhury S, Sinha MP. Effects of Psidium guajava aqueous extract on testosterone and serum lipid profile of albino rats. Middle-East J Sci Res 2014;21:1893-7.
Setty BS. Regulation of Epididymal function and sperm maturation-endocrine approach to fertility control in male. Endocrinology 1979;74:100-17.
Chinoy NJ, Mehta D. Effects of protein supplementation and deficiency on fluoride-induced toxicity in reproductive organs of male mice. Fluoride 1999;32:204-14.
Thejashwini MS, Krishna RH, Shivabasavaiah. Reversible antifertility effect of cyamposis psoralioides in male swiss albino mice. Int J Adv Biotechnol Res 2012;2:657-5.
Waterman MR, Keeney DS. Genes involved in androgen biosynthesis and the male phenotype. Horm Res 1992;38:217–21.
Ghosh D, Das UB, Misro M. Protective role of alpha-tocopherol-succinate (provitamin-E) in cyclophosphamide-induced testicular gametogenic and steroidogenic disorders: a correlative approach to oxidative stress. Free Radical Res 2002;36:1209–18.
Adhikary P, Banerji J, Choudhuri D, Das AK, Deb CC, Mukherjee SR, et al. Effect of Piper betle Linn. (Stalk) extract on male fertility. Indian J Pharmacol 1990;22:145-9.
Brokaw CJ. Direct measurements of sliding between outer doublet microtubules in swimming sperm flagella. Science 1989;243:1593-6.
Sharma N, Jacob D. Antifertility investigation and toxicological screening of the petroleum ether extract of the leaves of Mentha arvensis L. in male albino rats. J Ethnopharmacol 2001;75:5-12.
Kusemiju TO, Osinubi AA, Noronha CC, Okanlawon AO. Effect of aqueous extract of the bark of Carica papaya on the testicular histology in sprague-dawley rats. Nigerian Quarterly J Hospital Med 2010;20:133-7.
Cheng CY, Wong EW, Yan HH, Mruk DD. Regulation of spermatogenesis in the microenvironment of the seminiferous epithelium: new insights and advances. Mol Cell Endocrinol 2010;315:49–56.
Patil SJ, Satishagouda S, Vishwanatha T, Saraswati B. Effect of Terminalia bellirica barks extracts on activities of reproductive accessory ducts in male rats. Int J Pharm Sci Rev Res 2010;1:75-9.
Gonzales GF, Villena A. True corrected seminal fructose level: a marker of the function of seminal vesicles in infertile men. Int J Androl 2001;24:255-60.
Dawson EB, Harris WA, Teter MC, Powell LC. Effect of ascorbic acid supplementation on the sperm quality of smokers. Fertil Steril 1992;58:1034-9.