DEVELOPMENT OF SPECTROPHOTOMETRIC METHODS FOR THE ANALYSIS OF FLORFENICOL IN BULK AND DOSAGE FORMS

Authors

  • Mohamed M. Elimam Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, Sudan
  • Shaza W. Shantier Department Of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, Sudan
  • Elrasheed A. Gadkariem Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, Sudan
  • Magdi A. Mohamed Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, Sudan

Keywords:

Florfenicol, Spectrophotometry, dosage form

Abstract

Objectives: The objective of this research was to develop simple, sensitive and accurate zero ( °D), first (1D) and second (2D) order derivative spectrophotometric methods for the analysis of florfenicol in bulk and dosage forms.

Methods: The original UV spectrum (zero-order) of florfenicol aqueous solution was measured at 267 nm against its blank. This spectrum was then differentiated instrumentally to generate the first and second derivative spectra which were measured at 274 nm and 281 nm, respectively. The developed methods were validated as per ICH guidelines.

Results: Regression data of the developed methods obeyed Beer's law over the concentration range 3˗15µg/ml with a good correlation coefficient (not less than 0.998). Limits of detection were found to be 0.68, 1.30, 1.13µg/ml and limits of quantification were 2.05, 3.87, 3.58µg/ml for °D, 1D and 2D order derivative, respectively. The developed methods demonstrated good inter-day and intra-day precision at the three modes. The obtained recovery percentage (98.3±1.8%; n=3) reflected the freedom from interference by the excipients.

Conclusion: The statistical validation at 95% confidence level proves the sensitivity, accuracy and precision of the developed methods.

Keywords: Florfenicol, Spectrophotometry, Derivative spectra, Dosage form

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References

Syriopoulou VP, Harding AL, Goldmann DA, Smith AL. "In vitro antibacterial activity of fluorinated analogs of chloramphenicol and thiamphenicol." Antimicrob Agents Chemother 1981;19:294–7.

Gaunt PS, Langston C, Wrzesinski C, Gao D, Adams P, Crouch L, et al. "Multidose pharmacokinetics of orally administered florfenicol in the channel catfish". J Vet Pharmacol Ther 2012;36:502–6.

Susan S, Moslem J. Selective solid-phase extraction using molecular imprinted polymer sorbent for the analysis of Florfenicol in food samples. Food Chem 2013;141:1242–51.

Pengjie L, Xia C, Chunlai L, Hua K, Liming L, Zhigang J, et al. Simultaneous determination of thiamphenicol, florfenicol and florfenicol amine in swine muscle by liquid chromatography–tandem mass spectrometry with immunoaffinity chromatography clean-up. J Chromatogr B: Anal Technol Biomed Life Sci 2010;878:207–12.

Pengjie L, Xingyuan C, Zhanhui W, Haiyang J, Suxia Z, Xia C, et al. Development of an enzyme-linked immunosorbent assay for the detection of florfenicol in fish feed. Food Agric Immunol 2009;20:57-65.

Pfenning AP, Madson MR, Roybal JE, Turnipseed SB, Gonzales SA, Hurlbut JA, et al. Simultaneous determination of chloramphenicol, florfenicol, and thiamphenicol residues in milk by gas chromatography with electron capture detection. J AOAC Int 1998;81:714-20.

Ozez D, Senel H. Determination of lisinopril in pharmaceutical preparation by derivative UV spectrophotometry. J Pharm Biomed Anal 1999;21:691-5.

Anthony CM, Widdop B, Moss MS. Clarck’s isolation and identification of drugs, the pharmaceutical press: 2nd ed. UK; 2006.

El-Walily A, El-Gindy A, Bedair MF. Application of first derivative UV-spectrophotometry, TLC-densitometry and liquid chromatography for the simultaneous determination of mebeverine hydrochloride and sulphiride. J Pharm Biomed Anal 1999;21:535-48.

Nevin Erk. Assay of ephedrine hydrochloride and theophylline in pharmaceutical formulations by differential derivative spectroscopy. J Pharm Biomed Anal 2001;23:2555-61.

El-Gindy A, Ashour A, Abdel-Fattah L, Shabana MM. First derivative, TLC-densitometric and HPLC determination of acebutolol HCl in the presence of its acid-induced degradation product. J Pharm Biomed Anal 2001;24:527-34.

Shantier SW, Gadkariem EA. Spectrophotometric and HPLC methods for the determination of cefquinome sulphate in bullk and dosage forms. Elix Pharm 2013;59:15471-3.

Kavitha J, Saidevaraj AB, Lakshmi KS. UV Spectrophotometric estimation of sunitinib malate in pharmaceutical dosage form. Int J Pharm Pharm Sci 2016;8:99-103.

Elimam MM, Shantier SW, Gadkariem EA, Mohamed MA. Derivative spectrophotometric methods for the analysis and stability studies of colistin sulphate. J Chem 2015. Doi.org/10.1155/2015/624316.

Miller JC, Miller JN. Statistics and chemometrics for analytical chemistry. 5th ed. Pearson Education Limited, London, UK; 2005.

Published

01-05-2016

How to Cite

Elimam, M. M., S. W. Shantier, E. A. Gadkariem, and M. A. Mohamed. “DEVELOPMENT OF SPECTROPHOTOMETRIC METHODS FOR THE ANALYSIS OF FLORFENICOL IN BULK AND DOSAGE FORMS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 5, May 2016, pp. 347-9, https://journals.innovareacademics.in/index.php/ijpps/article/view/10709.

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