STANDARDIZATION OF A POLYHERBAL PREPARATION (POLâ€“6) FOR TREATMENT OF OXIDATIVE, INFLAMMATORY AND IMMUNE DISORDERS
Objective: The objective of this study was to develop and standardize a polyherbal preparation (POLâ€“6) and evaluate its use in treating oxidative, inflammatory and immune disorders.
Methods: The individual hydroalcoholic extractions of Hypericum perforatum, Bacopa monnieri, Centella asiatica, Withania somnifera, Ocimum sanctum and Camellia sinesis dried plant materials were done, and POLâ€“6 was prepared by mixing them in (1:2:2:2:2:1) proportions. POLâ€“6 has been standardized on the basis of organoleptic properties, physical characteristics, and physicochemical properties. The antioxidant activities were evaluated using free radical scavenging methods for 1,1â€“diphenylâ€“2â€“picrylhydrazyl, (DPPH) and nitric oxide assays. Antiâ€“inflammatory activity was done by albumin denaturation and HRBC membrane stabilization assays. Cell viability assay was used for immunomodulatory activity measurement by cultivating peripheral blood mononuclear cells.
Results: The results obtained from physical and chemical parameters evaluation shows that the contents of preparation present are within permissible limits. In inâ€“vitro antioxidant studies POLâ€“6 showed significant antioxidant activity when tested in 1â€“1 Diphenylâ€“2â€“picrylhydrazyl and nitric oxide assays. In inâ€“vitro antiâ€inflammatory investigation, POLâ€“6 exhibited significant inhibition against albumin denaturation and significant protection against membrane stabilization assay. POLâ€“6 also exhibit significant immunomodulatory activity at low doses, when tested in cell viability assay.
Conclusion: The results from this study rationalize the medicinal use of POLâ€“6 in oxidative, inflammatory and immune disorders.
Keywords: Polyherbal preparation, Antioxidant, Antiâ€inflammatory, Immunomodulatory
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