A RAPID, SENSITIVE AND VALIDATED ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY AND TANDEM MASS SPECTROMETRY METHOD FOR DETERMINATION OF PAROMOMYCIN IN MICE PLASMA: APPLICATION TO PHARMACOKINETIC STUDY


M. Jakir S. K. Pinjari, Rahul S. Somani, Ritu M. Gilhotra

Abstract


Objective: To develop and validate simple, sensitive, accurate and selective UPLC-MS/MS method for quantification of paromomycin (PARO) in mice plasma.

Methods: Precipitation method was used for the extraction of plasma samples, an aliquot of 25 µl plasma samples was extracted using 10% perchloric acid in water. Chromatographic separation was performed using waters acquity ultra-performance liquid chromatography (UPLC) columns, BEH HILIC (50 mm× 2.1 mm, 1.7 µm) by a gradient mixture of acetonitrile and water (both containing 0.005% v/v trifluro acetic acid) as a mobile phase at the flow rate of 0.2 ml/min. The analyte was protonated in the positive electrospray ionization (ESI) interface and detected in multiple reactions monitoring (MRM) modes using the transition m/z 308.60-455.30.

Results: The method had a short chromatographic run time of 3 min. Calibration curves were linear over wide ranges of 50.51-5019.22 ng/ml. The between and within-batch precision and accuracy of the method was determined by using 4 quality control samples, the highest % CV observed was 11.06. The mean recovery values are 78.17, 101.17 and 92.58 at low, medium and high-quality control levels; respectively.

Conclusion: It was concluded that the developed and validated UPLC-MS/MS method was rapid, sensitive, accurate, precise, linear, and specific. Therefore, this method can be used for quantification of PARO in mice plasma with various advantages over the reported methods.


Keywords


Paromomycin, Quantification, Pharmacokinetics, UPLC-MS/MS

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About this article

Title

A RAPID, SENSITIVE AND VALIDATED ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY AND TANDEM MASS SPECTROMETRY METHOD FOR DETERMINATION OF PAROMOMYCIN IN MICE PLASMA: APPLICATION TO PHARMACOKINETIC STUDY

Keywords

Paromomycin, Quantification, Pharmacokinetics, UPLC-MS/MS

DOI

10.22159/ijpps.2017v9i5.11024.

Date

01-05-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 5, 2017 Page: 86-90

Online ISSN

0975-1491

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99 Views | 66 Downloads

Authors & Affiliations

M. Jakir S. K. Pinjari
Department of Research, School of Pharmacy, Suresh Gyanvihar University, Mahal Jagatpura, Jaipur, Rajasthan 302017
India

Rahul S. Somani
Department of Research, School of Pharmacy, Suresh Gyanvihar University, Mahal Jagatpura, Jaipur, Rajasthan 302017
India

Ritu M. Gilhotra
Department of Research, School of Pharmacy, Suresh Gyanvihar University, Mahal Jagatpura, Jaipur, Rajasthan 302017


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