CARDIO PROTECTIVE EFFECT OF TAMARINDUS INDICA. LINN AGAINST ISOPROTERENOL INDUCED MYOCARDIAL INFARCTION IN RATS
Keywords:
Isoproterenol, Tamarindus indica, Antioxidant, Myocardial infarction, Electrocardiography, Cardiac markersAbstract
Objective: The aim of the current study was to evaluate the cardioprotective activity of Tamarindus Indica. Linn in Isoproterenol hydrochloride-induced Myocardial Infarction in rats.
Methods: In vitro antioxidant activity of aqueous fruit extract (AQFE), alcoholic fruit extract (ALFE), aqueous seed extract (AQSE) and alcoholic seed extract (ALSE) of TI was carried by well-established in vitro methods. The extracts were investigated for in vivo cardioprotective activity against IPRT induced myocardial infarction where the animals were treated with the extracts (250 mg and 500 mg/kg body weight) for 30 d. Standard vitamin E (100 mg/kg body weight) was used to compare the effect of the extracts. Change in body weight, ECG, heart weight, serum parameters, myocardial infarct area and enzyme estimation in heart homogenate were assessed.
Results: ALSE, AQSE and ALFE showed potent antioxidant activity with an IC50 of 12.94±0.14, 252.49±0.47 and 647.39±1.15 μg/ml. ALSE 250 mg and 500 mg showed dose-dependent cardioprotective activity in cardiotoxic rats. IPRT treated animals showed a marked increase in serum marker enzymes, an increase in the percent infarction area increase in heart weight and a decrease in body weight along with the increase in endogenous enzyme levels were observed.
Conclusion: The administered extracts significantly reversed the effects caused by IPRT. ALSE 250 and 500 mg/kg body weight showed maximum reversal while the other extracts AQSE, ALFE and AQFE were observed to have good antioxidant activity in vitro.
Keywords: Isoproterenol, Tamarindus indica. Linn, Antioxidant, Myocardial infarction, Electrocardiography, Cardiac markers
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