MULTIPLE TREATMENT OF EREMURUS HIMALAICUS EXTRACTS AMELIORATES CARBON TETRACHLORIDE INDUCED LIVER INJURY IN RATS

  • Ahlam Mushtaq university of kashmir
  • Mubashir Hussain Masoodi university of kashmir
  • Adil Farooq Wali university of kashmir
  • Bashir Ahmad Ganai University of Kashmir

Abstract

Objective: Eremurus himalaicus Baker, an edible herb of North Western Himalayas, has not been scientifically assessed for hepatoprotective potential. The ethyl acetate extract (EHE), methanolic extract (EHM) and aqueous extract (EHA) of Eremurus himalaicus were therefore evaluated for potential hepatoprotective activity in Wistar strain albino rats.

Methods: Carbon tetrachloride (1.5 ml/kg) was employed as hepatotoxin and was given on Day 1 of the experiment. The extracts at a dose of 300 mg/kg bw (EHE, EHM and EHA) and the standard at a dose of 10 mg/kg bw (Liv 52) were given for following 7 d and the biochemical parameters (SGOT, SGPT, ALP, TP, bilirubin and UA) were estimated in order to assess the liver function. Moreover, the liver tissue samples were examined for histopathological changes.

Results: The results for serum biochemical analysis in rats showed a rise in SGOT, SGPT, ALP and bilirubin levels and a decrease in TP and UA levels upon giving hepatotoxin. The administration of the extracts and standard drug, for a period of 7 d, showed a significant decrease in SGOT, SGPT, ALP and bilirubin levels and an increase in TP and UA levels for EHM when compared to the toxic group. These results correlated well with the histopathological findings of liver for normal, toxic and extract treated groups. The EHM treatment decreased the extent of fat deposition and necrosis caused by CCl4. The results were almost similar to the standard drug Liv 52.

Conclusion: Collectively; the results indicate that EHM exhibits significant hepatoprotective activity against CCl4 induced hepatotoxicity.

Keywords: Biochemical, Hepatoprotective, Hepatotoxin, Histopathological, Liver

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Author Biographies

Ahlam Mushtaq, university of kashmir
Research scholar, dept of biochemistry
Mubashir Hussain Masoodi, university of kashmir
sr.asst. prof. dept of pharmaceutical sciences
Adil Farooq Wali, university of kashmir
research scholar, dept of pharmaceutical sciences
Bashir Ahmad Ganai, University of Kashmir

director center of research for development

and

head dept of env. sciences

References

1. Grattagliano I, Bonfrate L, Catia VD, Wang HH, Wang DQH, Portincasa P. Biochemical mechanisms in drug-induced liver injury. World J Gastroenterol 2009;15:4865-76.
2. Guntupalli M, Chandana V, Pushpangadan P, Shirwaiker AI. Hepatoprotective effects of rubiadin, a major constituent of rubia cordifolia Linn. J Ethnopharmacol 2006;103:484–90.
3. Schuppan D, Jia J, Brikhaus B, Hahn EG. Herbal products for liver disease: A therapeutic challenge for the new millennium. Heatology 1999;30:1099-104.
4. Guguloth S, Vivekanandan L, Singaravel S, Sheik HS, Thangavel S. Hepatoprotective activity of vitex negundo linn bark against chemical-induced toxicity in experimental rats. Pharmanest 2011;2:5–6.
5. Nayar M, Sastry A. Botanical Survey of India, Kolkata. Red Data Book of Indian Plants; 1987.
6. NPMASD-National policy and macro level action strategy on biodiversity. The government of India ministry of environment and forests New Delhi, Published: December; 1999.
7. Sharma OP. Threatened plants of Jammu region, North-West Himalaya and strategies for their conservation. State Forest Res Institute, Jammu, Jammu Kashmir 2008;11:37.
8. Satish K, Irshad AH. Wild edibles of kishtwar high altitude national Park in Northwest Himalaya, Jammu and Kashmir (India). Ethnobot Leaflets 2009;13:195-202.
9. Shailja T. Medicinal plants used by tribal inhabitants of Sirmour district, Himachal pardesh. Int J Sci Res 2011;2:125-7.
10. Dhiraj SR, Anjna DK. Traditional health practices by ‘kinners’-A tribe in alpine and sub-alpine himalayas of kinnaur (Himachal Pradesh), India, department of botany, Rajkiya Kanya Mahavidhalaya, Shimla (H. P.). Life Sci Leafl 2011;22:1048–55.
11. Navarro VJ, Senior JR. Drug-related hepatotoxicity. N Engl J Med 2006;354:731-9.
12. Domenicali M, Caraceni P, Giannone F, Baldassarre M, Lucchetti G, Quarta C. A novel model of CCl4-induced cirrhosis with ascites in the mouse. J Hepatol 2009;51:991-9.
13. Kucera O, Cervinkova Z, Lotkova H, Krivakova P, Rousar T, Muzakova V. Protective effect of adenosylmethionine against galactosamine-induced injury of rat hepatocytes in primary culture. Physiol Res 2006;55:551-60.
14. Ledda-Columbano GM, Coni P, Curto M, Giacomini L, Faa G, Oliverio S. Induction of two different modes of cell death, apoptosis and necrosis, in rat liver after a single dose of thioacetamide. Am J Pathol 1991;139:1099-109.
15. Rousar T, Kucera O, Krivakova P, Lotkova H, Kandar R, Muzakova V, et al. Evaluation of oxidative status in acetaminophen-treated rat hepatocytes in culture. Physiol Res 2009;58:239-46.
16. Akram E, Pejman M, Maryam B, Jalal Z. Hepatoprotective activity of cinnamon ethanolic extract against CCl4-induced liver injury in rats. EXCLI J 2012;11:495-507.
17. Chatterjee TK. Medicinal plants with hepatoprotective properties. Herbal Options. Books and Applied Allied (P) Ltd., Calcutta; 2000. p. 143.
18. Rubinstein D. Epinephrine release and liver glycogen levels after carbon tetrachloride administration. Am J Physiol 1962;203:1033-7.
19. Johnson DE, Kroening C. Mechanism of early carbon tetra chloride toxicity in cultured rat hepatocytes. Pharmacol Toxicol 1998;83:231–9.
20. Kaplowitz N, Aw TY, Simon FR, Stolz A. Drug-induced hepatotoxicity. Ann Intern Med 1986;104:826–39.
21. Drotman R, Lawhan G. Serum enzymes are indications of chemical-induced liver damage. Drug Chem Toxicol 1978;1:163–71.
22. Seevola D, Baebacini GM, Bona S. Flavonoids and hepatic cyclic monophosphates in liver injury. Boll Ins Sieroter Milan 1984;63:777–82.
23. Wegner T, Fintelmann V. Flavonoids and bioactivity. Wien Med Wochenschr 1999;149:241–7.
24. Shahjahan M, Sabitha KE, Jainu M, Devi CSS. Effect of Solanum trilobatum against carbon tetrachloride induced hepatic damage in albino rats. Indian J Med Res 2004;120:194-8.
25. Sheweita SA, El-Gabar MA, Bastawy M. Carbon tetrachloride-induced changes in the activity of phase II drug-metabolizing enzyme in the liver of male rats: role of antioxidants. Toxicology 2001;165:217-24.
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How to Cite
Mushtaq, A., M. H. Masoodi, A. F. Wali, and B. A. Ganai. “MULTIPLE TREATMENT OF EREMURUS HIMALAICUS EXTRACTS AMELIORATES CARBON TETRACHLORIDE INDUCED LIVER INJURY IN RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 9, Sept. 2016, pp. 24-27, doi:10.22159//ijpps.2016.v8i9.11237.
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