UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF TRAMADOL HYDROCHLORIDE AND ACECLOFENAC IN BULK AND TABLET DOSAGE FORM

Authors

  • Lydia Jeeboi Department of Quality Assurance, Oriental College of Pharmacy, Plot No. 3,4,5, Sector-2, Near Sanpada Railway Station, Sanpada, Navi Mumbai 400705 Maharashtra, India
  • Snehalatha Boddu

Keywords:

UV spectrophotometric method, Tramadol Hydrochloride, Aceclofenac, Simultaneous estimation, Tablet dosage form, Validation

Abstract

Objective: To develop a simple, economic and validated UV spectrophotometric method for the simultaneous estimation of aceclofenac and tramadol hydrochloride in bulk and tablet dosage form.

Methods: The UV spectrophotometric method for simultaneous estimation of aceclofenac and tramadol hydrochloride has been developed. Methanol and water in the ratio 60:40 was used as the solvent. Tramadol hydrochloride and aceclofenac showed maximum absorbance at wavelength 214.8 nm and 275.6 nm respectively.

Results: The developed method showed tramadol hydrochloride and aceclofenac to be linear in the concentration range of 5–30 µg/ml with a correlation coefficient of 0.998 and 0.999 respectively. The result of recovery studies for the tablet was found to be in the range of 98.125%-101.0% and 98.01%-98.36% for tramadol hydrochloride and aceclofenac respectively.

Conclusion: The results show that the developed UV spectrophotometric method is simple, economical, accurate, precise and repeatable.

Keywords: UV spectrophotometric method, Tramadol Hydrochloride, Aceclofenac, Simultaneous estimation, Tablet dosage form, Validation

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References

Tramadol Hydrochloride. The American society of health-system pharmacists; 2014.

Tramadol. Compound Summary for CID 3374. PubChem. Open Chemistry Database; 2005.

Indian Pharmacopoeia. Vol. 3. The government of India, Ministry of Health and Family Welfare Ghaziabad: The Indian Pharmacopoeia Commission; 2010. p. 2245.

Grond S, Sablotzki A. Clinical pharmacology of tramadol. Clin Pharmacokinet 2004;43:879-923.

Grau M, Grauch JL, Montero A, Felipe A, Julia S. Pharmacology of the potent new non-steroidal anti-inflammatory agent aceclofenac. Arzneim Forsch 1991;41:1265-76.

Indian Pharmacopoeia. Vol. 2. The government of India, Ministry of Health and Family Welfare Ghaziabad: The Indian Pharmacopoeia Commission; 2010. p. 770.

Brogden RN, Wiseman LR. Aceclofenac: a review of its pharmacodynamic properties and therapeutic potential in the treatment of rheumatic disorders and in pain management. Drugs 1996;52:113–24.

Patel M, Purohit Z, Minal R, Meshram DB. UV spectrophotometric method for estimation of tramadol in bulk and tablet formulation by the area under curve method. Int J Pharm Chem Sci 2014;3:141-4.

Gogulamudi L, Sanjana K. Development and validation of uv spectroscopic method for determination of tramadol hydrochloride in bulk and formulation. Int J Pharm Pharm Sci 2012;4:275-9.

Rajasekhar KK, Shankarananth V, Jyosthna P, Chowdary SPS, Reddy DP. Spectrophotometric method for the estimation of tramadol in bulk and capsule dosage forms. J Pharm Res 2011;4:386-7.

Nayak S, Singh V, Bhaskar V. Development and validation of uv spectrophotometric method for tramadol hydrochloride. World J Pharm Pharm Sci 2015;4:773-81.

Sayed M, Bapat G, Inamdar N. Development of uv spectrophotometric methods and validation for estimation of tramadol hydrochloride in bulk and tablet dosage form by absorbance maxima and area under the curve method. J Appl Pharm 2014;6:210-6.

http://www.pharmaresearchlibrary.com/simple-validated-uv-method-for-tramadol-hcl-in-bulk-and-its-capsules. [Last accessed on 10 Dec 2015].

Rathore GS, Basniwal PK, Suthar M, Gupta RN. Spectrophotometric estimation of tramadol hydrochloride in pharmaceutical dosage forms. Asian J Chem 2009;21:6111-5.

Rajitha B, Prashanthi S, Reddy KR, TuljaRani G. Extractive spectrophotometric determination of tramadol hydrochloride in pure and pharmaceutical dosage forms. Int J PharmTech Res 2011;3:114-7.

Setty KN, Ramachar T, Chakravarthy IE, Prabhavathi K. A simple spectrophotometric estimation of tramadol hydrochloride in pharmaceutical formulations. Chem Sci Trans 2012;1:317-20.

Sagarbechara, Prabhu PP, Subrahmanyam EVS, Kaneria JJ, Saparia S, Shabaraya AR. Development of new analytical method and its validation for the determination of tramadol hydrochloride in bulk and marketed formulations. Asian J Biomed Pharm Sci 2013;3:53-6.

Kanakapura BV, Hosakere DR, Abdulrahman SAM. Sensitive spectrophotometric determination of tramadol hydrochloride in pharmaceuticals using folin-Ciocalteu's reagent. Turk J Pharm Sci 2013;10:57-68.

Setty NK, Prabhavathi K, Chakravarthi IE, Manjula GM. Spectrophotometric determination of tramadol hydrochloride in pharmaceutical formulations. Chem Sci Rev Lett 2013;1:168-71.

Abdellatef HE. Kinetic spectrophotometric determination of tramadol hydrochloride in a pharmaceutical formulation. J Pharm Biomed Anal 2002;29:835-42.

Kumar A, Nanda S, Chomwal R. Spectrophotometric methods for the estimation of tramadol hydrochloride in tablet formulation. Indian Pharm 2010;8:85-7.

Ravindra N. Spectrophotometric methods for quantitative estimation of tramadol hydrochloride from tablet formulation. Indo Am J Pharm Sci 2014;1:333-6.

Deepa P, Leeja K, Rajpandi R, Babu G. Development of a validated spectrofluorimetric method for the quantitative estimation of tramadol hydrochloride in bulk and pharmaceutical dosage form. Int J Am BMS 2013;2:101â€11.

Desai P, Captain A, Kamdar S. Development and validation of hptlc method for estimation of tramadol hcl in bulk and in capsule dosage form. Int J PharmTech Res 2012;4:1261-5.

Meyyanathan, Kumar P, Suresh B. Analysis of tramadol in pharmaceutical preparations by high-performance thin layer chromatography. J Sep Sci 2003;26:1359-62.

Dhumal BR, Bhusari KP, Patra A, Thareja S, Jain NS. Stability indicating high performance thin layer chromatographic method for the determination of tramadol hydrochloride in pharmaceutical formulation. J Liq Chromatogr Relat Technol 2015;38:1088-93.

Kanakapura BV, Hosakere DR, Xavier CM, Pavagada JR, Madihalli SR. A stability indicating uplc method for the determination of tramadol hydrochloride: application to pharmaceutical analysis. Chrom Res Int 2012. Doi.org/10.1155/2012/870951. [Article in Press]

Rao MM, Kumar EG, Madhu M, Sushmitha A, Tiruvengadarajan VS, Gopinath C. Development and validation of rp-hplc method for the assay of tramadol hcl in its capsule formulation. Indian J Adv Plant Res 2014;5:326-31.

Kumar SNP, Gowda DGB, Mantelingu K, Rangappa KS. Development and validation of hplc method for determination of tramadol hydrochloride in solid dosage form. J Pharm Res 2012;5:1438.

Zaheer Z, Devdikar MM, Nikalje AG. Stability indicating hplc method for determination of tramadol hydrochloride in tablet dosage form. Inventi Impact: Pharm Anal Qual Assur 2011;2:57.

Zaghloul, Iman Y, Radwan, Mahasen A. High-performance liquid chromatographic determination of tramadol in pharmaceutical dosage forms. J Liq Chromatogr Relat Technol 1997;20:779-87.

Saeed H, Tavakkoli N, Vahid N, Khani H. Determination of tramadol by dispersive liquid–liquid microextraction combined with GC–MS. J Chromatogr Sci 2015;53:655-61.

Aysel K, Yucel K, Duygu E. Electrochemical determination of tramadol in ampoule dosage forms by cyclic voltammetry. Asian J Chem 2010;22:159-67.

Shah R, Magdum C, Patil SK, Chougule DK, Naikwade N. Validated spectroscopic method for estimation of aceclofenac from tablet formulation. Res J Pharm Technol 2008;1:430-2.

Bose A, Dash PP, Sahoo MK. Simple spectrophotometric methods for estimation of aceclofenac from bulk and formulations. Pharm Methods 2010;1:57-60.

Golhar MK, Joshi RR, Gupta KR, Wadodkar SG. Development and validation of spectrophotometric methods for determination of aceclofenac in tablets. Int J ChemTech Res 2011;3:786-90.

Aderibigbe SA, Adegoke OA, Idowu OS, Olaleye SO. Sensitive spectrophotometric determination of aceclofenac following azo dye formation with 4-carboxyl-2,6-dinitrobenzene diazonium ion. Acta Poloniae Pharm Drug Res 2012;69:203-11.

Susmitha A, Kalarani HD, Venkatesh P, Reddy RK. Analytical method development and validation of aceclofenac in pharmaceutical dosage dorm by uv spectroscopy technique. Int J Pharm Pharm Sci 2013;5:150-3.

Sherikar OD, Puranik MP, Yeole PG. Spectrophotometric methods for determination of aceclofenac in bulk and its pharmaceutical dosage form. J Appl Chem Res 2011;16:15-20.

Valambhia KR. Validation of uv spectroscopic method of analysis for assay and dissolution of aceclofenac tablet. World J Pharm Pharm Sci 2013;2:3977-83.

Deepali G, Pallavi S, Chandrakant R, Chottaram S, Kundan P, Pandurang D. Validated spectroscopic method for estimation of aceclofenac from tablet formulation. Asian J Res Chem 2010;3:87-9.

Sharma RK, Bhaskar R, Arora R, Radhika B. Validated spectrophotometric method for estimation of aceclofenac in bulk drug and solid dosage forms. Int J Drug Formulation Res 2011;2:303-11.

Sherikar OD, Puranik MP, Yeole PG. A validated reversed phase-high performance liquid chromatographic (rp-hplc) method for simultaneous estimation of aceclofenac drug substance and its related traces impurities in the solid dosage form. Int J PharmTech Res 2011;3:547-54.

Paul K, Nagarajan JSK, Chandan RS. Standardization an a rp-hplc method for the estimation of aceclofenac in dosage form. Int J Res Pharm Chem 2011;1:853-9.

Jat Rk, Chhipa Rc, Sharma S. Rp-Hplc method for the estimation of aceclofenac in tablet dosage form. Int J Drug Res Technol 2013;1:8.

Nimisha SM, Suganthi A, Manjula D. Development of validated stability indicating hptlc method for aceclofenac in bulk drug and formulations. Int J Pharm Sci Rev Res 2012;13:78-81.

Suganthi A, Mathew NS, Manjuladevi AS, Ramu N, Ravi TK. Development of validated stability indicating hptlc method and its application to the assay of formulation and accelerated stability studies of aceclofenac. Am J PharmTech Res 2013;3:574-83.

William H, Parimala Devi B, Kurien J, Valsalakumari PK, Mohandas CK. Validated high performance thin layer chromatographic estimation of aceclofenac in bulk and pharmaceutical dosage forms. Int J Sci Res 2014;3;2613-7.

Patel TP, Prajapati AM. Spectrophotometric estimation of tramadol hydrochloride and aceclofenac in combined dosage form by second order derivative method. Am J PharmTech Res 2013;3:999-1009.

Snehalatha B, Momin M, Mishal AV, Kale TR. Development and validation of spectrophotometric method for simultaneous estimation of Nigella sativa seed oil and ginger extract in the same dosage form. Int J Pharm Sci Res 2014;5:5235-9.

ICH guidelines, Validation of analytical procedures: text and methodology, Q2A (R1) Nov; 2005.

Published

01-05-2016

How to Cite

Jeeboi, L., and S. Boddu. “UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF TRAMADOL HYDROCHLORIDE AND ACECLOFENAC IN BULK AND TABLET DOSAGE FORM”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 5, May 2016, pp. 334-40, https://journals.innovareacademics.in/index.php/ijpps/article/view/11332.

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