IN VITRO DEMONSTRATION OF DALBERGIA SISSOO (INDIAN ROSEWOOD) METHANOLIC EXTRACTS AS POTENTIAL AGENTS FOR SUNSCREENING AND DNA NICK PREVENTION
Keywords:Dalbergia sissoo, phytochemicals, antioxidants, SPF, pBluescript, Fenton, H2O2 and UV
Objective: This report is an attempt to evaluate methanolic extracts of leaves, fruits and bark from Dalbergia sissoo for photoprotective and DNA protective potential in vitro and to get an insight into its phytochemical constituents.
Methods: Total methanolic extracts of leaves, fruits and bark from D. sissoo were examined for total phenolics content (TPC), total flavonoids content (TFC) and antioxidant activity. Extracts alone, as well as part of gel formulations, were used to assess their sun protection factor (SPF) by spectrophotometry. Potential of extracts to prevent plasmid DNA damage by hydroxyl radicals generated through Fenton and ultraviolet+hydrogen peroxide (UV+H2O2) system was checked by agarose gel electrophoresis. GC-MS analysis was done to detect the presence of bioactive compounds in active fractions.
Results: TPC, TFC, antioxidant activity and SPF values of bark methanolic extract (BME) were found to be highest among the three extracts. Dose-dependent protection of DNA was observed in the extracts. Phytol, lupeol, squalene, palmitic acid and phytosterol etc. were the key constituent of the extracts.
Conclusion: As per the available literature, this is the first time exploration of D. sissoo methanolic extract as photoprotective and DNA protective agents. Total extracts or its purified fractions can be exploited as Cosmeceutical ingredient.
Keywords: Dalbergia sissoo, Phytochemicals, Antioxidants, SPF, pBluescript, Fenton, H2O2 and UV
Dutra EA, Oliveira DA, Kedor-Hackmann ER, Santoro MI. Determination of sun protection factor (SPF) of sunscreens by ultraviolet spectrophotometry. Rev Bras CiÃªnc Farm 2004;40:381-5.
Dixit P, Chillara R, Khedgikar V, Gautam J, Kushwaha P, Kumar A, et al. Constituents of Dalbergia sissoo Roxb. leaves with osteogenic activity. Bioorg Med Chem Lett 2012;22:890-7.
Vasudeva N, Vats M. Anti-spermatogenic activity of ethanol extract of Dalbergia sissoo Roxb. stem bark. J Acupuncture Meridian Studies 2011;4:116-22.
Yadav H, Yadav M, Jain S, Bhardwaj A, Singh V, Prakash O, et al. Antimicrobial property of a herbal preparation containing Dalbergia sissoo and Datura stramonium with cow urine against pathogenic bacteria. Int J Immunopathol Pharmacol 2008;21:1013-20.
Hajare SW, Chandra S, Sharma J, Tandan SK, Lal J, Telang AG. Anti-inflammatory activity of Dalbergia sissoo leaves. Fitoterapia 2001;72:131-9.
Sarg T, Ateya AM, Abdel-Ghani A, Badr W, Shams G. Phytochemical and pharmacological studies of Dalbergia sissoo growing in Egypt. Pharm Biol 1999;37:54-62.
Rahman MS, Begum B, Chowdhury R, Rahman KM, Rashid MA. Preliminary cytotoxicity screening of some medicinal plants of Bangladesh. Dhaka Univ J Pharm Sci 2008;7:47-52.
Asif M, Kumar A. Anti-inflammatory activity of ethanolic extract of Dalbergia sissoo (roxb.) Bark. Malay J Pharm Sci 2009;7:39-50.
Marwat SK, Fazal-Ur-Rehman MA, Ahmad M, Zafar M, Ghulam S. Medicinal folk recipes used as traditional phytotherapies in district Dera Ismail Khan, KPK, Pakistan. Pak J Bot 2011;43:1453-62.
Niranjan SP, Dharmendra S, Kiran P. Antidiabetic activity of ethanolic extract of Dalbergia sissoo L. Leaves in Alloxan-Induced diabetic rats. Int J Curr Pharm Res 2010;2:24-7.
Ashawat MS, Shailendra S, Swarnlata S. Biochemical and histopathological studies of herbal cream against UV radiation-induced damage. Trends Med Res 2007;3:135-41.
Atrooz OM. The antioxidant activity and polyphenolic contents of different plant seeds extracts. Pak J Biol Sci 2009;12:1063-8.
Svobodova A, Psotova J, Walterova D. Natural phenolics in the prevention of UV-induced skin damage: a review. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2003;147:137-45.
Afaq F, Mukhtar H. Botanical antioxidants in the prevention of photocarcinogenesis and photoaging. Exp Dermatol 2006;15:678-84.
Kaur CD, Saraf S. Photochemoprotective activity of alcoholic extract of Camellia sinensis. Int J Pharmacol 2011;7:400-4.
Caunii A, Pribac G, Grozea I, Gaitin D, Samfira I. Design of optimal solvent for extraction of bioâ€“active ingredients from six varieties of Medicago sativa. Chem Cent J 2012;6:123-30.
Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965;16:144-58.
Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199-200.
Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant powerâ€: the FRAP assay. Anal Biochem 1996;239:70-6.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 1999;26:1231-7.
Mansur JS, Breder MNR, Mansur MCA, Azulay RD. DeterminaÃ§Ã£o do fator de proteÃ§Ã£o solar por espectrofotometria. An Bras Dermatol 1986;61:121-4.
Gordon VC. Evaluation du facteur de protection solaire. Parfums Cosmet Aromes 1993;112:62-5.
Walters C, Keeney A, Wigal CT, Johnstom CR, Cornelius RD. The spectrophotometric analysis and modeling of sunscreens. J Chem Educ 1997;74:99-102.
Fourneron JD, Faraud F, Fauneron A. Sur la mesure in vitro de la protection solaire de crÃ¨mes cosmÃ©tiques. C R Acad Sci II 1999;2:421-7.
Pissavini M, Ferrero L, Alaro V, Heinrich U, Tronnier H, Kockott D, et al. Determination of the in vitro SPF. Cosmet Toiletries 2003;118:63-72.
Mahajan UN, Wasule DD. Sunscreen and antioxidant activities of herbal gel formulations. Pharmacogn Mag 2008;4:99-101.
Sayre RM, Agin PP, Levee GJ, Marlowe E. Comparison of in vivo and in vitro testing of sunscreen formulas. Photochem Photobiol 1979;29:559-66.
Lee S, Kim EK, Hwang JW, Oh HJ, Cheong SH, Moon SH, et al. Purification and characterization of an antioxidative peptide from enzymatic hydrolysates of duck processing by-products. Food Chem 2010;123:216-20.
Guha G, Rajkumar V, Mathew L, Kumar RA. The antioxidant protection potential of Indian tribal medicinal plants. Turk J Biol 2011;35:233-42.
Hatano T, Edamatsu R, Hiramatsu M, Moti A, Fujita Y, Yasuhara T, et al. Effects of tannins and related polyphenols on superoxide anion radical, and on 1, 1-diphenyl-2-picrylhydrazyl radical. Chem Pharm Bull 1989;37:2016-21.
Nichols JA, Katiyar SK. Skin photoprotection by natural polyphenols: anti-inflammatory, antioxidant and DNA repair mechanisms. Arch Dermatol Res 2010;302:71-83.
Chen CH, Lee O, Yao CN, Chuang MY, Chang YL, Chang MH, et al. Novel azulene-based derivatives as potent multi-receptor tyrosine kinase inhibitors. Bioorg Med Chem Lett 2010; 20:6129-32.
Guarrera M, Turbino L, Rebora A. The antiâ€inflammatory activity of azulene. J Eur Acad Dermatol Venereol 2001;15:486-7.
Roll A, Reich K, Boer A. Use of fumaric acid esters in psoriasis. Indian J Dermatol Venereol Leprol 2007;73:133-7.
Aparna V, Dileep KV, Mandal PK, Karthe P, Sadasivan C, Haridas M. Antiâ€inflammatory property of nâ€hexadecanoic acid: structural evidence and kinetic assessment. Chem Biol Drug Des 2012;80:434-9.
Hua KF, Hsu HY, Su YC, Lin IF, Yang SS, Chen YM, et al. Study on the antiinflammatory activity of methanol extract from seagrass Zostera japonica. J Agric Food Chem 2006;54:306-11.
Smith TJ. Squalene: potential chemopreventive agent. Expert Opin Invest Drugs 2000;9:1841-8.
Huang ZR, Lin YK, Fang JY. Biological and pharmacological activities of squalene and related compounds: potential uses in cosmetic dermatology. Molecules 2009;14:540-54.
Santos CC, Salvadori MS, Mota VG, Costa LM, de Almeida AA, de Oliveira GA, et al. Antinociceptive and antioxidant activities of phytol in vivo and in vitro models. Neurosci J 2013:1-9. Doi.org/10.1155/2013/949452. [Article in Press]
Saleem M. Lupeol, a novel anti-inflammatory and anti-cancer dietary triterpene. Cancer Lett 2009;285:109-15.
Raman BV, La S, Saradhi MP, Rao BN, Krishna AN, Sudhakar M, et al. Antibacterial, antioxidant activity and GC-MS analysis of Eupatorium odoratum. Asian J Pharm Clin Res 2012;5:99-106.
KrÃ³l SK, KieÅ‚bus M, Rivero-MÃ¼ller A, Stepulak A. Comprehensive review on betulin as a potent anticancer agent. BioMed Res Int 2015:1-11. Doi.org/10.1155/2015/584189. [Article in Press]
Evans MD, Dizdaroglu M, Cooke MS. Oxidative DNA damage and disease: induction, repair and significance. Mutat Res Rev Mutat Res 2004;567:1-61.
Markesbery WR, Lovell MA. DNA oxidation in Alzheimer's disease. Antioxid Redox Signaling 2006;8:2039-45.
Valko M, Rhodes CJ, Moncol J, Izakovic MM, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 2006;160:1-40.
Goldstein S, Meyerstein D, Czapski G. The fenton reagents. Free Radical Biol Med 1993;15:435-45.
Leba LJ, Brunschwig C, Saout M, Martial K, Vulcain E, Bereau D, et al. Optimization of a DNA nicking assay to evaluate Oenocarpus bataua and Camellia sinensis antioxidant capacity. Int J Mol Sci 2014;15:18023-39.
Cataldo F. Hydrogen peroxides photolysis with different UV light sources including a new UV-led light source. New Front Chem 2014;23:99-110.
Wink DA, Wink CB, Nims RW, Ford PC. Oxidizing intermediates generated in the Fenton reagent: kinetic arguments against the intermediacy of the hydroxyl radical. Environ Health Perspect 1994;102:11-5.