EVALUATION OF THE ANTILEISHMANIAL EFFICACY OF MEDICINAL PLANT CHENOPODIUM ALBUM LINN. AGAINST EXPERIMENTAL VISCERAL LEISHMANIASIS.

Authors

  • Rupinder Kaur
  • Jaspreet Kaur
  • Sukhbir Kaur
  • Jyoti Joshi Department of Zoology, Panjab University, Chandigarh.

Keywords:

Visceral leishmaniasis, Chenopodium, BALBc, DTH

Abstract

Objective: Visceral leishmaniasis is a neglected tropical disease resulting in death if not properly treated. The ongoing search for better leishmanicidal compounds has brought herbal drugs in to the limelight as safe and effective substitutes to conventional therapies which have various drawbacks. The current study was designed to evaluate the antileishmanial efficacy of medicinal plant Chenopodium album Linn. against L donovani in inbred BALB/c mice.

Methods: Inbred BALB/c mice were infected intravenously with 1x107 Leishmania donovani promastigotes and Kept for 30 days. These animals were then treated with two doses (500mg/kg body wt. and 1000mg/kg body wt.) of methanolic extracts of C. album  orally for 7 days.

Results: The animals treated with methanolic extracts of C. album, revealed a significant reduction in parasite load. These animals also showed a heightened DTH response and increased IgG2a as an indicator of protective Th1 type of immune response. Moreover, the liver and kidney function tests were found to be in the normal range.

Conclusion: Hence the drug proved to be a good antileishmanial but further studies recommended before it is to be tested in higher animal models.

 

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References

Marinho DS, Casas CNPR, Pereira CCdA, Leite IC. Health Economic Evaluations of Visceral Leishmaniasis Treatments: A Systematic Review. PLoS Negl Trop Dis, 2015, 9: e0003527.

Lal CS, Verma RB, Verma N, Siddiqui NA, Rabidas VN, Pandey K, et al. Hypertriglyceridemia: a possible diagnostic marker of disease severity in visceral leishmaniasis. Infection. 2016, 44:39-45.

Croft SL, Sundar S, Fairlamb AH. Drug resistance in leishmaniasis. Clin Microbiol Rev. 2006, 19: 111–126.

De Mesquita, M.L, Desrivot J, Bories C, Fournet A, Paula JE, Grellier P, Espindola LS. Antileishmanial and trypanocidal activity of Brazilian Cerrado plants. Mem Inst Oswaldo Cruz. 2005, 100: 783-787.

Rocha L G, Almeida J R, Macpdo RO, Barbosa-Filho JM. A review of natural products with antileishmanial activity. Phytomedicine. 2005,12: 514-535.

Mutiso JM, Macharia JC, Kiio MN, Ichagichu JM, Rikoi H, Gicheru MM. Development of Leishmania vaccines: predicting the future from past and present experience. J Biomed Res. 2013, 27: 85–102.

Sen R, Chatterjee M. Plant derived therapeutics for the treatment of Leishmaniasis. Phytomedicine. 2011, 18: 1056-69.

Salem, Manar M, Werbovetz and Karl A. Natural products from plants as drug candidates and lead compounds against leishmaniasis and trypanosomiasis. Curr Med Chem. 2006, 13: 2571-2598.

Bhattacharjee SK. Handbook of medicinal plants, Pointer Publishers, Jaipur, 3rd Edn. 2001,352:1-2.

Kaur C, Kapoor HC Antioxidant activity and total phenolic content of some asian vegetables. J food Sci Technol. 2002, 37:153-161.

Kumar R, Mishra AK, Dubey NK, Tripathi TB. Evaluation of Chenopodium album oil as a potential source of antfungal, antiaflatoxigenic and antioxidant activity. Int J Food Microbiol. 2007, 115: 159-164.

Cutilo F, D’Abrosca B, Dellagreca M, Marino CD, Golino A, Previtera L, Zarrelli A. Cinnamic acid amides from chenopodium album:effects on seed germination and plant growth. Phytochemistry. 2003, 64:1381-1387.

Cutilo F, Della Greca M, Gionti M, Previtera L, Zarrelli A. Phenols and ligans frm Chenopodium album. Phytochem Anal. 2006,17:344-349.

Lavaud C, Voutquenne L, Bal P and Pouny I. Saponins from Chenopodium album. Fitoterapia. 2000, 71:338-340.

Dellagreca M, Previtera L, Zarrelli A. A new xyloside from Chenopodium album. Nat Prod Res. 2005, 19: 87-90.

Khoobchandani M, Ojeswu Bk, Sharma B, Srivastava MM. Chenopodium album prevents progression of cell growth and enhances cell toxicity in human breast cancer cell lines. Oxid Med Cell Longev. 2009, 2:160-165.

Nigam V, Parakash PM. Hepatoprotective activity of Chenopodium album Linn. Against alcohol induced liver damage. Int J Phytomed. 2011, 3: 511-523.

Joshi J, Kaur S. To evaluate the curative potential of single dose of cisplatin in combination with immunotherapy in inbred BALB/c mice against visceral leishmaniasis. International journal of biopharmaceutics. 2015, 6: 13-17.

Bradley DJ, Kirkley J. Regulation of Leishmania populations within the host. I. the variable course of Leishmania donovani infections in mice. Clin Exp Immunol. 1977, 30:119-29.

Joshi J, Kaur S. A comparative evaluation of efficacy of chemotherapy, immunotherapy and immunochemotherapy in visceral leishmaniasis-an experimental study. Parasitol Int. 2014, 63:612-20.

Salam MA. Prospects of vaccine in leishmaniasis. Bangladesh J Med Microbiol. 2009, 3: 40-46.

Makwali JA, Wanjala FME, Kaburi JC, Ingonga J, Byrum WW and Anjili CO. Combination and monotherapy of Leishmania major infection in BALB/c mice using plant extracts and herbicides. J Vector Borne Dis. 2012, 49: 123-130.

Singh N, Kumar A, Gupta P, Chand K, Samant M, Maurya R and Dube A. Evaluation of antileishmanial potential of Tinospora sinensis against experimental visceral leishmaniasis. Parasitol Res. 2008, 102: 561-565.

Mahmoudzadeh-Nikam H, Kiaei SS and Iravani D. Leishmania tropica infection in comparison to Leishmania major, induces lower delayed type hypersensitivity in BALB/c mice. Korean J parasitol. 2007, 45(2):103-109.

Silva VMG, de-Araújo CF, Navarro IC, Oliveira PRS, Pontes–de-Carvalho L. Enhancement of experimental cutaneous leishmaniasis by Leishmania extract: identification of a disease-associated antibody specificity. BMC Research Notes. 2015, 8:197.

Launois P, Swihart Kg, Milon G and Louis JA. Early production of IL-4 in susceptible mice infected with Leishmania major rapidly induces IL-12 unresponsiveness. J immunol. 1997,158: 3317-3324.

Kaur S, Kaur G, Sachdeva H and Kaur J. In vivo evaluation of the antileishmanial activity of two immunomodulatory plants, Emblica officinalis and Azadirachta indica in BALB/c mice. Int. J. Ayurvedic Herbal Med. 2013, 3: 1066-1079.

Published

01-07-2016

How to Cite

Kaur, R., J. Kaur, S. Kaur, and J. Joshi. “ AGAINST EXPERIMENTAL VISCERAL LEISHMANIASIS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 7, July 2016, pp. 227-31, https://innovareacademics.in/journals/index.php/ijpps/article/view/11726.

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Original Article(s)