ANTI-INFECTIVE COATING OF GENTAMICIN SULPHATE ENCAPSULATED PEG/PVA/CHITOSAN FOR PREVENTION OF BIOFILM FORMATION
Keywords:
Biofilm, Biomedical implant, Silicone, Biocompatibility, Cytotoxicity, Drug encapsulation, HemocompatibilityAbstract
Objective: The main aim of the current study is to show Anti-infective coating on biomedical implants, one of the novel approaches to control the biofilm formation.
Methods: In this study, the coating was employed by the slurry of Polyvinyl alcohol (PVA),Polyethylene glycol (PEG) and Chitosan with encapsulated Gentamicin Sulphate (GS) using Slurry-dipping technique on Silicon. Two different strategies were followed to coat the Silicone namely Coating I with chitosan and Coating II without chitosan. Drug encapsulation efficiencies of Coating I and Coating II were found to be 86.8% and 83.96% respectively.
Results: In vitro drug release profile of Coating I and Coating II in phosphate buffer saline at pH 7.4 for 48 hrs were found to be 30.3µg/ml and 24.2 µg/ml respectively. Antimicrobial activity of coated samples against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli showed effective results. Biofilm formation was investigated by XTT reduction assay which showed reduction of biofilm on coated sample with respect to the uncoated sample. Biocompatibility of coated sample was confirmed by MTT assay against SW-71 trophoblast cell-lines and hemolysis test against fresh human blood. Surface morphological analysis using SEM confirmed the uniform coating and AFM showed the average surface roughness reduction in coated sample.
Conclusion: The present study indicated that GS encapsulated PVA, PEG and Chitosan cross linked polymer coated Silicone can be potentially used for production of Silicone based implant.
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