• Bardees Mickky Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt
  • Muhammad Abbas Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt
  • Omar El-shhaby Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt


Objective: The present study addresses the effect of water deficit stress on the antimicrobial capacity of alfalfa (Medicago sativa) plants.

Methods: Methanolic extracts of alfalfa plants grown in different soil types, varying in sand proportion, either alone or combined with various levels of water regimes were assessed for antibacterial and antifungal activities following cup plate method. The phytochemical profiles of plant extracts were also qualitatively screened using appropriate chemical reagents. Moreover, data were intensively processed via two different statistical designs.

Results: Increasing sand amount induced the inhibitory effect of plant extracts on Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Salmonella typhi, Mucor circinelloides, Rhizopus azygosporus and R. microsporus with less pronounced action on Shigella flexneri, Staphylococcus epidermidis, Candida albicans and Emericella quadrillineata; as well as a reversed influence on Pseudomonas aerugenosa and Streptococcus pyrogenes. Furthermore, withholding irrigation water enhanced the plant suppressive action on E. coli, Salmonella typhi, Staphylococcus epidermidis, Candida albicans and R. microsporus with less marked or reversed influence on the other tested microbes. However, Pseudallescheria ellipsoidea, two species of Penicillium and five of Aspergillus could resist the studied plant extracts. The results also revealed that the extracts of water-unsatisfied plants generally contained higher amounts of alkaloids, amino acids, flavonoids, glycosides, phytosterols, saponins, steroids, tannins, terpenoids and reducing sugars.

Conclusion: The employed biological evaluations point out to promising antimicrobial efficiency of alfalfa plants particularly when stressed.

Keywords: Alfalfa, Sand, Drought, Antibacterial, Antifungal, Phytochemicals


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1. Nakashima K, Yamaguchi-Shinozaki K, Shinozaki K. The transcriptional regulatory network in the drought response and its crosstalk in abiotic stress responses including drought, cold, and heat. Front Plant Sci 2014;5:1-7.
2. Rezaeieh KAP, Gürbüz B, Uyanık M. Biotic and abiotic stresses mediated changes in secondary metabolites induction of medicinal plants. Tıbbi ve Aromatic Bilgiler Sempozyumui 2012;13:218-22.
3. Selmar D. Potential of salt and drought stress to increase pharmaceutical significant secondary compounds in plants. Agric and Forestry Res 2008;58:139-44.
4. Subhas CM, Harsha R, Dinesha R, Thammanna SS. Antibacterial activity of Coleus aromaticus leaves. Int J Pharm Pharm Sci 2010;2:63-6.
5. Bora KS, Sharma A. Phytochemical, and pharmacological potential of Medicago sativa: a review. Pharm Biol 2011; 49:211-20.
6. El-Desoukey RM. Phytochemical and antimicrobial activity of Medicago sativa (alfalfa) as a source of animal food against some animal pathogens. Glob Veter 2015;14:136-41.
7. Kosem N, Han YH, Moongkarndi P. Antioxidant and cytoprotective activities of methanolic extracts from Garcinia mangostana Hulls. Sci Asia 2007;33:283-92.
8. Nair R, Chanda S. Antibacterial activity of some medicinal plants against some medically important bacterial strains. Indian J Pharmacol 2006;38:142-4.
9. Harborne JB. editor. Phytochemical methods: a guide to modern techniques of plant analysis, 3rd edition. New York: Chapman and Hall Int Ed; 1998.
10. Kokate CK. editor. Pharmacohnosy, 16th edition. India: Nirali Prakasham; 2001.
11. Djeussi DE, Noumedem JAK, Seukep JA, Fankam AG, Voukeng IK, Tankeo SB, et al. Antibacterial activities of selected edible plants extracts against multidrug-resistant Gram-negative bacteria. Complementary Altern Med 2013;13:164-71.
12. Ramakrishna A, Ravishankar GA. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal Behav 2011;6:1720-31.
13. Bonjean K, De Pauw-Gillet MC, Defresne MP, Colson P, Houssier C. The DNA intercalating alkaloid cryptolepine interferes with topoisomerase II and inhibits DNA synthesis in B16 melanoma cells primarily. Biochemistry 1998;37:1536-46.
14. Cushnie TP, Lamb AJ. Antimicrobial activity of flavonoids. Int J Antimicrob Agents 2005;26:343-56.
15. Donova MV. Transformation of steroids by actinobacteria: a review. Appl Biochem Microbiol 2007;43:1-14.
16. Cheeke PR. Actual and potential applications of Yucca schidigera and Quillaja saponaria saponins in human and animal nutrition. J Anim Sci 2000;77:1-10.
17. Ya C, Gaffney SH, Lilley TH, Haslam E. Carbohydrate polyphenol complexation. In: Hemingway SRW, Karchesy JJ. editors. Chemistry and significance of condensed tannins. New York: Plenum Press; 1988. p. 553.
18. Mendoza L, Wilkens M, Urzua A. Antimicrobial study of the resinous exudates and of diterpenoids and flavonoids isolated from some Chilean Pseudognaphalium (Asteraceae). J Ethnopharmacol 1997;58:85-8.
19. Reddy CUM, Jayakar B, Srinivasan R. Synthesis and antimicrobial activity of α-n-phthilimido and acetimido derivatives from amino acids and anhydrides. Int J Pharma Bio Sci 2010;1:81-6.
20. Dhalel DA, Markandeya SK. Antimicrobial and phytochemical screening of Plumbago zeylanica Linn. (Plumbaginaceae) leaf. J Exp Sci 2011;2:4-6.
21. Molnár J, Gunics G, Mucsi I, Koltai M, Petri I, Shoyama Y, et al. Antimicrobial and immunomodulating effects of some phenolic glycosides. Acta Microbiol Hung 1989;36:425-32.
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How to Cite
Mickky, B., M. Abbas, and O. El-shhaby. “ECONOMIC MAXIMIZATION OF ALFALFA ANTIMICROBIAL EFFICACY USING STRESSFUL FACTORS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 9, Sept. 2016, pp. 299-03, doi:10.22159/ijpps.2016v8i9.12160.
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