DESIGN OF HYDROQUINONE INDICATOR STRIP BASED ON POLYMETHYLMETHACRYLATE (PMMA) FOR IDENTIFICATION OF HYDROQUINONE IN WHITENING COSMETICS: CASE STUDY IN BANDUNG INDONESIA

  • Yunita Kurniati
  • Anisa Desy Aryanti
  • Danni Ramdhani
  • Driyanti Rahayu
  • Mutakin .
  • Aliya Nur Hasanah Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Padjadjaran University, Sumedang, West Java, Indonesia

Abstract

Objective: The objective of this research was to developing an indicator strips made from polymer polymethylmethacrylate (PMMA) to detect hydroquinone in whitening cosmetics.

Methods: The strips were prepared by reagent blending method with a polymer PMMA that mixed with a chemical reagent for hydroquinone based on chemical structure: ferric chloride (FeCl3), Benedict, and ammoniacal silver nitrate. PMMA were varied with concentrations of 5%, 7.5%, and 10%. The ratios of ethyl acetate and the reagents were off 6:4; 7:3; and 8:2.

Results: The results showed that the following concentration was an optimum mixture of chemical reagent-polymer and ethyl acetate FeCl3-PMMA 7.5% 7:3; benedict-PMMA 5% 6:4; and ammoniacal silver nitrate-PMMA 5% 7:3. 22 samples of whitening cream were not contained hydroquinone based on strip indicator analysis. These results are appropriate with a confirmatory test using UV spectrophotometry and thin layer chromatography. The indicator strips showed a positive result in detect the samples that had been spiked by hydroquinone but has a limitation when applied to cream dosage forms. The limit of detection (LOD) and stability of the indicator strip FeCl3-PMMA 7.5% 7:3 was 20.05 mg/l and stable up to 157 d, Benedict-PMMA 5% 6: 4 was 0177 mg/l and stable up to 123 d, and ammoniacal silver nitrate-PMMA 5% 7:3 was 0.025 mg/l and stable up to 174 d. The selectivity test results showed that indicator strip did not give false positive results.

Conclusion: Based on the tests, the indicator strip has limitations to detect hydroquinone in whitening cream cosmetics that required further modifications to the detection process.

Keywords: Hydroquinone, UV-Vis spectrophotometry, Thin Layer Chromatography (TLC), Indicator strip, Polymethylmethacrylate (PMMA)

Keywords: Hydroquinone, UV-Vis spectrophotometry, Thin Layer Chromatography (TLC), Indicator strip, polymethylmethacrylate (PMMA).

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How to Cite
Kurniati, Y., A. D. Aryanti, D. Ramdhani, D. Rahayu, M. ., and A. N. Hasanah. “DESIGN OF HYDROQUINONE INDICATOR STRIP BASED ON POLYMETHYLMETHACRYLATE (PMMA) FOR IDENTIFICATION OF HYDROQUINONE IN WHITENING COSMETICS: CASE STUDY IN BANDUNG INDONESIA”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 7, July 2016, pp. 354-9, https://innovareacademics.in/journals/index.php/ijpps/article/view/12161.
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