IN VITRO ASSESSMENT OF PRASARANI SANDHAN, A TRADITIONAL POLYHERBAL AYURVEDIC MEDICINE, FOR IMMUNOSTIMULATING ACTIVITY IN SPLENIC CELLS OF BALB/C MICE

  • Md. Moklesur Rahman Sarker Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Tsushima-naka, Kita-ku, Okayama 7008530, Japan, Diabetes, Obesity and Genomics Research Group, Clinical Investigation Centre, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • Hasan Imam Department of Pharmacy, PrimeasiaUniversity, 9 Banani C/A, Dhaka-1213
  • M Salahuddin Bhuiyan Department of Pharmacy, Faculty of Biological Science, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh
  • M Shahabuddin Kabir Choudhuri Department of Pharmacy, Faculty of Biological Science, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

Abstract

Objective: The objective of the present study was to evaluate the immunostimulating potential of an Ayurvedic preparation Prasarani Sandhan (PRS) by measuring immunoglobulinM (IgM) production and splenocytes proliferation in vitro.

Methods: Freshly prepared BALB/c mice splenocytes were treated with 0.25, 0.5, 0.75, 1, 1.5, 2, 3, and 4% (v/v) of PRS and the cells were sub-cultured at 37°C, humidified atmosphere containing 5% CO2 for 120 hours. IgM production and cells proliferation were determined by enzyme-linked immunosorbent assay (ELISA) and 3-(4,5-dimethylthiazol-2-y)-2,5-diphenylterazolium bromide (MTT) methods, respectively. The presence of endotoxin was determined by treating PRS with polymyxin B in culture.

Results: PRS at the doses of 0.25, 0.5, 0.75, 1, 1.5% (v/v) significantly promoted polyclonal IgM productions (1.847, 2.024, 1.986, 1.958, and 1.465 µg/mL, respectively) compared to control (0.358 µg/mL). Similarly, 0.25, 0.5, 0.75, and 1% (v/v) of the preparation significantly stimulated splenocytes proliferation (absorbance 0.346, 0.394, 0.385, and 0.378 respectively, at 570 nm) compared to control (Abs. 0.156). The highest increment of IgM production and splenocytes proliferation by 0.50% PRS was 5.65 and 2.52-times than control. Polymixin B treated PRS significantly reduced its ability for IgM production and cells proliferation by 51.5% and 30%, respectively. Thus, the findings suggest that the apparently observed enhancement of IgM by PRS was due to the contamination of bacterial endotoxin, PRS has no immunostimulating property.

Conclusion: This is the first report on the immunostimulating activity of PRS. Prasarani Sandhan was contaminated with bacterial endotoxin and the preparation failed to exhibit immunostimulating activity in vitro.

 

Keywords: Prasarani Sandhan, Ayurvedic medicine, Immunostimulant, Splenocytes, Immunoglobulin M, Differentiation, Proliferation, BALB/c mice.

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Author Biography

Md. Moklesur Rahman Sarker, Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Tsushima-naka, Kita-ku, Okayama 7008530, Japan, Diabetes, Obesity and Genomics Research Group, Clinical Investigation Centre, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia

Clinical Investigation Centre, Faculty of Medicine, University of Malaya

Post Doctoral Visiting Research Fellow and Principal Investigator of a High Impact Research (HIR) Project

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Sarker, M. M. R., H. Imam, M. S. Bhuiyan, and M. S. K. Choudhuri. “IN VITRO ASSESSMENT OF PRASARANI SANDHAN, A TRADITIONAL POLYHERBAL AYURVEDIC MEDICINE, FOR IMMUNOSTIMULATING ACTIVITY IN SPLENIC CELLS OF BALB/C MICE”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 9, 1, pp. 531-4, https://innovareacademics.in/journals/index.php/ijpps/article/view/1315.
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