THE PROTECTIVE ROLE OF FLAXSEED LIGNAN IN MALE RABBITS WITH HIGH-FAT DIET: HISTOPATHOLOGICAL STUDY
Keywords:Flaxseed Lignan, Secoisolariciresinol, Diglucoside
Objective: The objective of the study was to investigate the effect of dietary flaxseed-derived lignan supplement as anti-hyperlipidemic (fatty liver changes) on rabbit liver.
Methods: Rabbits were assigned randomly into three groups (five rabbits for each group): group-1, normal diet (negative control); group-2, 1.6 % cholesterol diet for 30 d (positive control) and group-3, 1.6 % cholesterol for 30 d then gave 40 mg/kg/day of pure flax lignan for 14 d.
Results: Marked proliferation of fibrous connective tissue infiltrated with mononuclear cells was recorded; congested dilated of sinusoids and fibrosis in the portal area around the proliferation of bile ducts and congested portal blood vessels with the proteinous material in the lumen of bile ducts were also recorded. In other sections, the liver showed large necrotic area replacement with RBCs as well as enlarged foamy hepatocytes with mononuclear cells in narrowing sinusoids were seen.
Conclusion: From the results of this study we can conclude that lignan has been used successfully in decreasing the inflammatory cells in congested blood vessels and sinusoids and has decreased the inflammatory cells infiltration in moderate fibrosis in the portal area around the bile ducts of rabbit liver tissue.
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