DECOLOURIZATION OF TRIPHENYLMETHANE DYES AND DYE INDUSTRY EFFLUENT BY STAPHYLOCOCCUS AUREUS ISOLATED FROM DYE CONTAMINATED SITE
Objective: The objectives of the present study were a) to isolate and screen bacteria for dye removal from synthetic solution b) to optimize various variables such as pH, static/shaking and initial dye concentration on degradation of triphenyl methane dyes namely basic violet 3 and basic green 4 by isolated Staphylococcus aureus c) to analyse enzymes involved in the biodegradation of triphenylmethane dyes d) to treat real leather dyeing wastewater with newly isolated strain of Staphylococcus aureus e) to characterize untreated and treated leather dyeing wastewater f) to study the effects of real and treated effluent on plants and Rhizobium.
Methods: Isolation of bacteria from sludge was carried out by spread plate method and the bacteria was identified by morphological and biochemical characterization. The isolated bacterium was screened for dye decolorization potential of triphenylmethane dyes basic violet 3 and basic green 4 The effects of parameters were studied by varying pH (from 3 to 9), temperature (from 15-45 Â°C), and initial dye concentration (from 10-500 mg/l). The enzyme involved in biodegradation was studied in intracellular extract. Real leather dyeing wastewater was treated with the bacteria and characterized. The treated wastewater was tested on plants and Rhizobium for toxicity.
Results: Dye decolorization potential of bacteria Staphylococcus aureus isolated from wastewater for leather dyes basic violet 3 and basic green 4 were evaluated. Dye decolorization using bacteria was found to be dependent on physicochemical parameters (shaking, pH and initial dye concentration). Enzymes NADH-DCIP reductase and MG reductase were found to play dominant role during biodegradation of synthetic dyes. Application oriented studies using growing bacteria in pure cultures were carried out with leather dyeing wastewater collected from DKS prime tanners. Analysis of raw leather dyeing wastewater showed high pollution load in terms of color, Total solids, Total suspended solids, Total dissolved solids and Biological oxygen demand whereas the leather dyeing wastewater treated with pure culture of Staphylococcus aureus showed considerable decrease in Total solids, Total suspended solids, Total dissolved solids and Biological oxygen demand values which were within the permissible limits. Phytotoxicity and microbial toxicity studies confirmed the non-toxic nature of treated leather dyeing wastewater.
Conclusion: Our study proved that Staphylococcus aureus can serve as a potential remediation agent for the treatment of leather dyeing wastewater.
2. Babu BR, Parande AK, Raghu S, Prem Kumar T. Cotton textile processing: waste generation and effluent treatment. J Cotton Sci 2007;11:141-53.
3. Asamudo NU, Daba AS, Ezeronye OU. Bioremediation of textile effluent using Phanerochaete chrysosporium. Afr J Biotechnol 2005;4:1548-53.
4. Banat IM, Nigam P, Singh D, Marchant R. Microbial decolorization of textile-dye containing effluents: a review. Bioresour Technol 1996;58:217-27.
5. Fu Y, Viraraghavan T. Fungal decolorization of dye wastewaters: a review. Bioresour Technol 2001;79:251â€“62.
6. Clarke EA, Anliker R. Organic dyes and pigments Handbook of Environmental Chemistry, Anthropogenic Compounds, Part A. Vol. 3. Springer, New York; 1980. p. 181â€“215.
7. Sani RK, Banerjee UC. Decolourization of triphenylmethane dyes and textile and dye-stuff effluent by Kurthia sp. Enzyme Microb Technol 1999;24:433-7.
8. An SY, Min SK, Cha IH, Choi YL, Cho YS, Kim CH, et al. Decolorization of triphenylmethane and azo dyes by Citrobacter sp. Biotechnol Lett 2002;24:1037-40.
9. Gill PK, Arora DS, Chander M. Biodecolourization of azo and triphenylmethane dyes by Dichomitus squalens and Phlebia spp. J Ind Microbiol Biotechnol 2002;28:201â€“3.
10. Sharma DK, Saini HS, Singh M, Chimni SS, Chadha BS. Isoloation and characterization of microorganisms capable of decolorizing various triphenylmethane dyes. J Basic Microbiol 2004;44:59â€“65.
11. Ren S, Guo J, Zeng J, Sun G. Decolorization of triphenylmethane, azo and anthraquinone dyes by a newly isolated Aeromonas hydrophila strain. Appl Microbiol Biotechnol 2006;72:1316-21.
12. Chen CC, Liao HJ, Cheng CY, Yen CY, Chung YC. Biodegradation of crystal violet by Pseudomonas putida. Biotechnol Lett 2007;29:391-6.
13. Cho BP, Yang T, Blankenship LR, Moody JD, Churchwell M. Synthesis and characterization of N-demethylated metabolites of malachite green and leuco malachite green. Chem Res Toxicol 2003;16:285-94.
14. Littlefield NA, Blackwell BN, Hewitt CC, Gaylor DW. Chronic toxicity and carcinogenicity studies of gentian violet in mice. Fundam Appl Toxicol 1985;5:902-12.
15. Decampo R, Moreno SN. The metabolism and mode of action of gentian violet, drug. Metab Rev 1990;22:161-78.
16. Slokar YM, Le Marechal AM. Methods of decolorization of textile wastewaters. Dyes Pigm 1998:37:335-56.
17. Wijetunga S, Xiufen L, Wenquan R, Chen J. Removal mechanisms of acid dyes of different chemical groups under anaerobic mixed culture. Ruhuna J Sci 2007;2:96-110.
18. McMullan G, Meehan C, Conneely A, Kirby N, Robinson T, Nigam P, et al. Mini-review: microbial decolorization and degradation of textile dyes. Appl Microbiol Biotechnol 2001;56:81-7.
19. Verma P, Madamwar D. Decolorization of synthetic dyes by a newly isolated strain of Serratia marcescens. World J Microbiol Biotechnol 2003;19:615-8.
20. Rai HS, Bhattacharyya MS, Singh J, Bansal TK, Vats P, Banerjee UC. Removal of dyes from the effluent of textile and dyestuff manufacturing industry: a review of emerging techniques with reference to biological treatment. Crit Rev Environ Sci Technol 2005;35:219â€“38.
21. Sathish S, Joshua A. Study on decolorization of dyestuff (azo dyeâ€“congo red) by using bacterial consortia. Int J Pharm Pharm Sci 2015;7:143-6.
22. Charumathi D. Removal of synthetic dyes from textile wastewater using yeast, Ph. D Thesis. VIT University; 2011. p. 62-78.
23. Salokhe MD, Govindwar SP. Effect of carbon source on the biotransformation enzymes in serratia marcescens. World J Microbiol Biotechnol 1999;15:229-32.
24. Jadhav JP, Govind was SP. Biotransformation of malachite green by Saccharomyces cerevisiae MTCC 463. Yeast 2006;23:315-23.
25. Saratale RG, Saratale GD, Chang HS, Govindwar SP. Decolorization and biodegradation of textile dye Navy blue HER by Trichosporon beigelii NCIM-3326. J Hazard Mater 2009;166:1421-8.
26. Manivanan R. Recycling of Industrial effluents, New India Publishing Agency, Pitam Pura, New Delhi; 2006.
27. Anon, Standard methods of water and wastewater examination. 18th Ed. American Public Health Association. NW Washington DC; 1992. p. 2-127.
28. CPCB. Pollution Control Acts, Rules, and Notification, New Delhi; 1998. p. 311-2.
29. Sarnaik S, Kanekar P. Bioremediation of color of methyl violet and phenol from a dye industry waste effluent using Pseudomonas spp. isolated from factory soil. J Appl Bacteriol 1995;79:459-69.