IN VITRO ANTI-OBESITY EFFECT OF MACROLICHENS HETERODERMIA LEUCOMELOS AND RAMALINA CELASTRI BY PANCREATIC LIPASE INHIBITORY ASSAY

  • Rashmi Shivanna Department of studies in Botany, Manasagangotri, University of Mysore, Mysore 570006, Karnataka, India
  • Hengameh Parizadeh Department of studies in Botany, Manasagangotri, University of Mysore, Mysore 570006, Karnataka, India
  • Rajkumar H. Garampalli Department of studies in Botany, Manasagangotri, University of Mysore, Mysore 570006, Karnataka, India

Abstract

Objective: Obesity is a chronic disorder caused by an imbalance between energy intake and expenditure in which excessive fat will be deposited in adipose tissue and poses a risk to the health and well-being of humans. Agents which inhibit pancreatic lipase play an important role in the treatment of obesity. The aim of this study was to assess the potential effect of macro lichens Heterodermia leucomelos (L.) Poelt a foliose lichen and Ramalina celastri (Sprengel) Krog and Swinscow a fruticose lichen in the treatment of obesity.

Methods: In vitro anti-obesity inhibitory effect of macro lichens were evaluated by using chicken pancreatic lipase activity. Lipase was extracted from the chicken pancreas. Different concentrations from 5-25 mg/ml of methanol and ethyl acetate extracts of lichens Heterodermia leucomelos and Ramalina celastri was incubated with pancreas lipase.

Results: With the increase in the concentration of extracts the higher inhibition of the enzyme was observed. Solvent methanol showed good activity compared to ethyl acetate. Percentage of inhibition ranged from 19.7-69.8 and 20.0-86.6 % in the methanol extract of Heterodermia leucomelos and Ramalina celastri respectively. Comparatively lichen Ramalina celastri in methanol extract showed maximum inhibition of 86.6 %, whereas ethyl acetate showed an inhibition of 63.0% at 25 mg/ml against enzyme lipase.

Conclusion: In the present study, the inhibitory activity of lichen indicates its protective role in treating obesity. Molecular sequencing of this lichen helps in future to determine the various metabolic pathways that are responsible for the production of novel compounds.

Keywords: Anti-obesity, Lichen, Lipase, Hyperlipidemia, Ramalina celastri

Downloads

Download data is not yet available.

References

1. Roh C, Jung U. Screening of crude plant extracts with anti-obesity activity. Int J Mol Sci 2012;13:1710-9.
2. Chantre P, Lairon D. Recent findings of green tea extract AR25 (Exolise) and its activity for the treatment of obesity. J Phytomed 2002;9:3–8.
3. Third Report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III) Final Report. Circulation 2002;106:3143-421.
4. Villa-Ruano N, Pacheco-Hernandez Y, Lara-Zaragoza EB, Franco-Monsreal J. Fitoterapia: alternativa para el control de la obesidad. Elementos 2011;84:21-5.
5. Kang JG, Park C. Anti-obesity drugs: a review about their effects and safety. Diabetes Metab J 2012;36:13-25.
6. Sharma N, Sharma VK, Seo S. Screening of some medicinal plants for anti-lipase activity. J Ethnopharmacol 2005; 97:453–6.
7. Birari RB, Bhutani KK. Pancreatic lipase inhibitors from natural sources: unexplored potential. Drug Discover Today 2007;12:879-89.
8. Lowe M. The triglyceride lipases of the pancreas. J Lipid Res 2002;43:2007-16.
9. Muller K. Pharmaceutically relevant metabolites from lichens. Appl Microbiol Biotechnol 2002;56:9-16.
10. Chand P, Singh M, Mayank R. Antibacterial activity of some Indian lichens. J Ecophysiol Occup Health 2009;9:23-9.
11. Karagoz A, Dogruoz N, Zeybek Z, Aslan A. Antibacterial activity of some lichen extracts. J Med Plants Res 2009;3:1034–9.
12. Awasthi DD. A key to the macro lichens of India and Nepal. J Hattori Bot Lab 1988;65:207–302.
13. Sahani KM, Khan IM, Chandan RC. Bovine pancreatic lipase: isolation, homogeneity and characterization. J Dairy Sci 1976;59:369-75.
14. Zheng C, Duan Y, Gao J, Ruan Z. Screening for anti-lipase properties of 37 traditional Chinese medicinal herbs. J Chin Med Assoc 2010;73:319-24.
15. Mukherjee M. Human digestive and metabolic lipases a brief review. J Mol Catal B: Enzym 2003;22:369–76.
16. Ong S, Paneerchelvan S, Lai H, Rao NK. In vitro lipase inhibitory effect of thirty-two selected plants in Malaysia. Asian J Pharm Clin Res 2014;7:19-24.
17. Adnyana IK, Sukandari EY, Yuniarto A, Finna S. Anti-obesity effect of the pomegranate leaves ethanol extract (Punica granatum L.) in high-fat diet induced mice. Int J Pharm Pharm Sci 2014;6:626-31.
18. Aruna A, Vijayalakshmi K, Karthikeyan V. Pancreatic lipase inhibitory screening of Citrullus lanatus leaves. Pharma Innovation J 2014;3:44-52.
19. Gupta N, Ganeshpurkar A, Jatav N, Bansal D, Dubey N. In vitro prevention of chick pancreatic lipase activity by Abroma augusta extract. Asian Pacific J Trop Biomed 2012;2(2 Suppl):S712-S715.
20. Anilkumar HS, Kekuda PTR, Vinayaka KS, Swathi D, Venugopal TM. Anti-obesity (Pancreatic lipase inhibitory) activity of Everniastrum cirrhatum (Fr.) Hale (Parmeliaceae). Pharma-cognosy 2011;3:65-8.
21. Park B, Lee C, Jang Y, Pyo S. Anti-obese effect of ramalin in high-fat-diet-induced obese mice. FASEB J 2014;28:Suppl 824, 7.
22. Fazio AT, Adler MT, Maier MS. Usnic acid and triacylglycerides production by the cultured lichen mycobiont of Ramalina celastri. Nat Prod Commun 2014;9:213-4.
23. Machado MJ, Gorin PA, Torri G, Iacomini M. The occurrence of glycolipids in the lichen Ramalina celastri. Braz J Med Biol Res 1994;27:523-6.
24. Machado MJ, Guerrini M, Gorin PAJ, Torri G, Lacomini M. A galactosphingolipid from the lichen, Ramalina celastri. Phytochemistry 1997;45:651-3.
25. Carneiro-Leão AMA, Buchi DF, Iacomini M, Gorin PAJ, Oliveira MBM. Cytotoxic effect againts HeLa cells of polysaccharides from the lichen Ramalina celastri. J Submicrosc Cytol Pathol 1997;29:503–9.
Statistics
275 Views | 870 Downloads
Citatons
How to Cite
Shivanna, R., H. Parizadeh, and R. H. Garampalli. “IN VITRO ANTI-OBESITY EFFECT OF MACROLICHENS HETERODERMIA LEUCOMELOS AND RAMALINA CELASTRI BY PANCREATIC LIPASE INHIBITORY ASSAY”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 9, no. 5, May 2017, pp. 137-40, doi:10.22159/ijpps.2017v9i5.13560.
Section
Original Article(s)