PREPARATION AND CHARACTERIZATION OF CURCUMIN LOADED CASSAVA STARCH NANOPARTICLES WITH IMPROVED CELLULAR ABSORPTION
Objectives: The present study describes loading of curcumin onto cassava starch nanoparticles to improve its cellular absorption.
Methods: Cassava starch nanoparticles were prepared by acid hydrolysis. A solution of curcumin in acetone was mixed thoroughly with the starch nanoparticles suspension and the loading efficiency was determined. The loaded nanoparticles were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC) and fluorescence spectra. Antioxidant activity of pure curcumin and curcumin loaded on nanostarch was studied. Cytotoxicity and cellular uptake of the curcumin were studied using L929 fibroblast cell lines.
Results: Curcumin was successfully loaded on starch nanoparticles as revealed by microscopic analyses. Antioxidant assay revealed that antioxidant activity was on par for pure curcumin and loaded curcumin as observed from the EC50 values. The result of cytotoxicity showed that curcumin incorporated starch nanoparticles were non-toxic to normal cells. The cellular absorption of curcumin, which was loaded on starch nanoparticles was significantly higher than that of pure curcumin.
Conclusion: Curcumin loaded on starch nanoparticles exhibited improved cellular absorption with non-toxicity and hence it can be potentially used in various pharmaceutical applications.
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