A NEW STABILITY-INDICATING RP-HPLC METHOD FOR DETERMINATION OF CURCUMIN: AN APPLICATION TO NANOPARTICULATE FORMULATION


Suchitra Panigrahi, Rajashree Hirlekar

Abstract


Objective: The present study was aimed to develop a rapid, accurate, linear, sensitive and stability-indicating high performance liquid chromatographic method for determination of curcumin and to implement the developed method for the estimation of curcumin in the nanoparticulate formulation.

Methods: Method development was performed using various solvent, buffer-solvent ratios, at different flow rates for better resolution and to decrease the run time. The developed method was validated in accordance with the international conference on harmonization (ICH) guidelines. The developed method was implemented to estimate the amount of curcumin in the curcumin-nanoparticulate formulation.

Results: The optimum chromatographic conditions with adequate resolution for curcumin (16.10 min) was achieved when the separation was carried using C18 column at ambient temperature with an isocratic elution mode of mobile phase composed of a degassed mixture of phosphate buffer pH 3 and acetonitrile (50:50 v/v) at 1.0 ml/min flow rate with a total run time of 20 min. The developed method was validated for system suitability, accuracy, precision, limit of detection (LOD), limit of quantitation (LOQ), linearity and range. The LOD and LOQ were found to be 0.018 and 0.056 μg/ml respectively, which indicates that the method was sensitive, and can detect and quantify at lower levels of curcumin. Linearity range was from 5-15 μg/ml for curcumin with regression coefficient 0.997 indicates that at this concentration range curcumin was highly linear. Percent assay of curcumin was found to be 98.7% and curcumin recovered was found to be 0.78 mg which are estimated by using the developed method.

Conclusion: The developed analytical method is simple, precise, and reproducible and thus can be used for stability-indicating analysis of curcumin in pharmaceutical formulations.


Keywords


Analytical method development, Curcumin, Nanoparticles, RP-HPLC-PDA, Stability-indicating

| HTML | PDF |

References


Korany MA, Haggag RS, Ragab MA, Elmallah OA. A validated stability-indicating HPLC method for simultaneous determination of silymarin and curcumin in various dosage forms. Arabian J Chem 2013;6:1-15.

Gugulothu DB, Patravale VB. A new stability-indicating HPLC method for simultaneous determination of curcumin and celecoxib at single wavelength: an application to the nanoparticulate formulation. Pharm Anal Acta 2012;3:1-6.

Sarvesh S, Gaurav KS, Vimal K. Development of spectro-photometric methods for simultaneous determination of artesunate and curcumin in a liposomal formulation. Int J Appl Pharm 2015;7:18-21.

Radhika G, Manoj KS, Gulab ST, Avinash S. Study of formulation, characterization and wound healing potential of transdermal patches of curcumin. Asian J Pharm Clin Res 2012;5:225-30.

Steffi PF, Srinivasan M. Curcumin, a potent anticarcinogenic polyphenol–a review. Asian J Pharm Clin Res 2014;7:1-8.

FDA Professional Drug Information Home Page. Available from: http://www.drugs.com. [Last accessed on 30 Mar 2015].

Basnet P, Basnet NS. Review curcumin: an anti-inflammatory molecule from a curry spice on the path to cancer treatment. Mol 2011;16:4567-98.

Ansari MJ, Ahmad S, Kohli K, Ali J, Khar RK. Stability-indicating HPTLC determination of curcumin in bulk drug and pharmaceutical formulations. J Pharm Biomed Anal 2005;39:132-8.

Lechtenberg M, Quandt B, Nahrstedt A. Quantitative determination of curcuminoids in curcuma rhizomes and rapid differentiation of curcuma domestica val. and curcuma xanthorrhiza roxb. by capillary electrophoresis. Phytochem Anal 2004;15:152-8.

Schiborr C, Eckert GP, Rimbach G, Frank J. A validated method for the quantification of curcumin in plasma and brain tissue by fast narrow-bore high-performance liquid chromatography with fluorescence detection. Anal Bioanal Chem 2010;397:1917-25.

Han YR, Zhu JJ, Wang YR, Wang XS, Liao YH. A simple RP-HPLC method for the simultaneous determination of curcumin and its prodrug, curcumin di-decanoate, in rat plasma and the application to pharmacokinetic study. Biomed Chromatogr 2011;25:1144-9.

Yadav VR, Sarasija S. A sensitive reversed-phase HPLC method for the determination of curcumin. Pharmacogn Mag 2009;5:71-4.

Moorthi C, Kathiresan K. Simultaneous estimation of curcumin and piperine using validated RP-HPLC-PDA method and its application in pharmaceutical nanoformulation. Int J Pharm Pharm Sci 2013;5:475-8.

Dandekar PP, Patravale VB. Development and validation of a stability-indicating LC method for curcumin. Chromatographia 2009;69:871-7.

Wang F, Hung W, Wang Y. Fluorescence enhancement effect for the determination of curcumin with yttrium(III)–curcumin–sodium dodecyl benzene sulfonate system. J Lumin 2008;128:110-6.

Goren AC, Cikrikci S, Cergel M, Bilsel G. Rapid quantitation of curcumin in turmeric via NMR and LC–tandem mass spectrometry. J Food Chem 2009;113:1239-42.

Zhao XL, Yang CR, Yang KL, Li KX, Yang HH, Chen WD. Preparation and characterization of nanostructured lipid carriers loaded traditional chinese medicine, zedoary turmeric oil. Drug Dev Ind Pharm 2010;36:773–80.

ICH guidance on method validation; validation of analytical procedures text and methodology Q2; 2005.




About this article

Title

A NEW STABILITY-INDICATING RP-HPLC METHOD FOR DETERMINATION OF CURCUMIN: AN APPLICATION TO NANOPARTICULATE FORMULATION

Keywords

Analytical method development, Curcumin, Nanoparticles, RP-HPLC-PDA, Stability-indicating

DOI

10.22159/ijpps.2016v8i12.14473

Date

01-12-2016

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 8, Issue 12, 2016 Page: 149-155

Online ISSN

0975-1491

Statistics

151 Views | 248 Downloads

Authors & Affiliations

Suchitra Panigrahi
Department of Quality Assurance, Vivekanand Education Society’s College of Pharmacy, Hashu Advani Memorial Complex, Behind Collector Colony, Chembur(E), Mumbai 400074. Maharashtra, India
India

Rajashree Hirlekar
Department of Quality Assurance, Vivekanand Education Society’s College of Pharmacy, Hashu Advani Memorial Complex, Behind Collector Colony, Chembur(E), Mumbai 400074. Maharashtra, India
India


Article Tools



Refbacks

  • There are currently no refbacks.