• Gouse Basha Sheik College of Applied Medical Sciences, Ad-Dawadmi, Shaqra University, Saudi Arabia
  • Muazzam Sheriff Maqbul Faculty of Microbiology and Immunology, Ibn Sina National College of Medical Sciences, Jeddah, Saudi Arabia
  • Gokul Shankar S. Faculty of Medicine, AIMST University, Kedah, Malaysia
  • Ranjith M S Faculty of Medicine, Quest International University, Perak, Ipoh, Malaysia


Objective: To isolate and characterize novel actinomycetes and to evaluate their antibacterial activity against drug-resistant pathogenic bacteria

Methods: In the present study, 19 soil samples were collected from different localities of Ad-Dawadmi, Saudi Arabia. Actinomycetes were isolated from these samples using serial dilution and plating method on Actinomycetes isolation agar supplemented with nalidixic acid and actidione to inhibit bacteria and fungi. Crude extracts of potential actinomycetes were produced by submerged fermentation. The antimicrobial activity of crude extracts of actinomycetes was tested against different bacteria using the agar well diffusion method. Characterization of the isolates was done by morphological, physiological and biochemical methods.

Results: A total of 9 (47%) isolates of actinomycetes were isolated from 19 different soil samples tested. Among them, 4 (44%) isolates confirmed as Streptomyces sp. showed potential antimicrobial activity against one or more test organisms. Crude extracts were made from these 4 actinomycetes isolates(DOM1, DOM3, DP3, DP4)and tested for their antibacterial activities against 4 different clinical bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus). Crude extract from DP3 isolate showed highest antibacterial activity against all the four test organisms (28 mm, 21 mm, 20 mm and 18 mm) respectively and DP4 showed lowest antibacterial activity against all the four test organisms (14 mm, 12 mm, 0 mm, 6 mm) respectively. The highest zone of inhibition was shown by DP3 against Staphylococcus aureus (28 mm) and Escherichia coli was resistant for DP4. Most of the Inhibition zones produced by crude extracts showed significant differences when compared with control, tested against test organisms (P<0.05). Inhibition zones produced by DP3 and DOM1 against Staphylococcus aureus were 28 mm and 23 mm, respectively which were strong active when compared with control Ciprofloxacin (18 mm).

Conclusion: Further studies for purification of bioactive metabolites and molecular characterization analysis of isolated Streptomyces sp. are in progress which would be helpful in discovering novel compounds of commercial value.

Keywords: Actinomycetes, Drug resistance, Antimicrobial activity, Streptomyces


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1. Adegboye MF, Babalola OO. Taxonomy and ecology of antibiotic-producing actinomycetes. Afr J Agric Res 2012;7:2255-61.
2. Yuan L, Zhang Y, Yu L, Sun C, Wei Y, Liu H, et al. Actinopolymorpha cephalotaxi sp. nov., a novel actinomycete isolated from rhizosphere soil of the plant Cephalotaxus fortune. Int J Syst Evol Microbiol 2010;60:51-4.
3. Berdy J. Bioactive microbial metabolites. J Antibiot 2005;58:1-26.
4. Poopal AC, Laxman RS. Studies on the biological reduction of chromate by Streptomyces griseous. J Haz Mat 2009;169:539-45.
5. Elardo PSM, Scheuermayer M, Kozytska S, Hentschel U. Streptomyces axinellae sp. isolated from the Mediterranean sponge. Axinellapolypoides (Porifera). Int J Syst Evol Microbiol 2009;59:1433-7.
6. Pandey B, Ghimire P, Agrawal VP. International Conference on the Great Himalayas: climate, health, ecology, management and conservation, Kathmandu; 2004.
7. Alanis AJ. Resistance to antibiotics: are we in the post-antibiotic era? Arch Med Res 2005;36:697-705.
8. Elliah P, Ramana T, Bapi Raju KVVS, Sujatha P, Uma Sankar AM. Investigation on marine actinomycetes from the bay of Bengal near Karnataka coast of Andhra Pradesh. Asian J Microbiol Biotechnol Environ Sci 2004;6:53-6.
9. Aghamirian RM, Ghiasian AS. Isolation and characterization of medically important aerobic actinomycetes in the soil of Iran. Open Microbiol J 2009;3:53-7.
10. Reddy NG, Ramakrishna DPN, Rajagopal SV. Morphological, physiological and biochemical studies of marine Streptomyces rochei (MTCC 10109) showing antagonistic activity against human pathogenic microorganisms. Asian J Biol Sci 2011;4:1-14.
11. Ramendra SP, Charu S, Pragati S, Ajay K. Isolation and screening of antimicrobial and extracellular pigment producing actinomycetes from Chambal Territory of Madhya Pradesh region, India. Asian J Pharm Clin Res 2016;9:157-60.
12. Nanjwade BK, Chandrashekhara S, Shamarez AM, Goudanavar SP, Manvi VF. Isolation and morphological characterization of antibiotic-producing actinomycetes. Trop J Pharma Res 2010;9:231-6.
13. Atta HM. An antifungal agent produced by Streptomyces olivaceis cleroticus, AZ-SH 514. World Appl Sci J 2009;6:1495-505.
14. Beena J, Joji R. Evaluation of antibacterial activity of the leaf and flower essential oils of Gliricidia sepium from South India. Int J Appl Pharm 2010;2:20-2.
15. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16:313-40.
16. Nolan R, Cross T. Isolation and screening of actinomycetes. In: Goodfellow M, Williams ST, Mordarski M. editors. In: Actinomycetes in Biotechnology. Academic Press: Inc, California; 1988. p. 1-32.
17. Sudha SKS, Hemalatha R. Isolation and screening of antibiotic-producing actinomycetes from the garden soil of Sathyabama University, Chennai. Asian J Pharm Clin Res 2015;8:110-4.
18. Ramendra SP, Charu S, Pragati S, Ajay K. Isolation and screening of antimicrobial and extracellular pigment producing actinomycetes from Chambal territory of Madhya Pradesh region, India. Asian J Pharm Clin Res 2016;9:157-60.
19. Kalyani ALT, Ramya SKM, Annapurna J. Isolation and characterization of antibiotic-producing actinomycetes from marine soil samples. Int J Curr Pharm Res 2012;4:109-12.
20. Sujatha P, Swethalatha P. Isolation and screening of novel Streptomyces from sediments of bay of Bengal near Srikakulam coast. Int J Curr Pharm Res 2016;9:40-4.
21. Midhun KD, Girijasankar G. Isolation, screening and characterization of antibiotic-producing actinomycetes from Kapuluppada plastic waste dumping yard, Visakhapatnam. Int J Pharm Pharm Sci 2016;8:221-9.
22. Sreejetha M, Divya, Veena S, Kokati VBR. The bioactive potential of Streptomycesvariabilis-DV-35 isolated from Thottada marine sediments, Kannur, Kerala. Asian J Pharm Clin Res 2016;9:67-71.
23. Kariminik A, Baniasadi F. Phage antagonistic activity of actinomycetes on some gram-negative and gram-positive bacteria. World Appl Sci J 2010;8:828-32.
24. Das S, Ward LR, Burke C. Screening of marine Streptomyces species. for potential use as probiotics in aquaculture. Aquaculture 2010;305:32–41.
25. Saleh MG, Jamal SMS, Amr AMH, Saleh AK, Dina AT, Mohamed MA. Isolation and identification of antimicrobial actinomycetes strain from Saudi environment. J Food Agric Environ 2014;12:1072-9.
26. Muharram MM, Abdelkader MS, Alqasoumi SI. Antimicrobial activity of soil actinomycetes isolated from Alkharj, KSA. Int Res J Microbiol 2013;4:12-20.
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How to Cite
Sheik, G., M. Maqbul, G. S., and R. S. “ISOLATION AND CHARACTERIZATION OF ACTINOMYCETES FROM SOIL OF AD-DAWADMI, SAUDI ARABIA AND SCREENING THEIR ANTIBACTERIAL ACTIVITIES”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 9, no. 10, Oct. 2017, pp. 276-9, doi:10.22159/ijpps.2017v9i10.15402.
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