• Priyaah Kumaran Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang, 26300 Pahang, Malaysia
  • Arun Gupta Centre for Biocomposite and Innovative Materials (CBIM) Universiti Malaysia Pahang, 26300 Pahang, Malaysia
  • Swati Sharma Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang, 26300 Pahang, Malaysia



Chicken feathers, Keratin, Eco-friendly, Hydrogel, Wound healing, FTIR


Objective: A novel cross-linked keratin hydrogel was prepared by integrating keratin from chicken feather into an aloe-vera, Chitosan and honey based dressing formulation separately.

Methods: Keratin fibres extracted from chicken feathers are eco-friendly, non-abrasive, biodegradable, insoluble in organic solvents and having good mechanical properties, hydrophobic behaviour, low density and finally cheap. Keratin based hydrogels were prepared with five types of ingredients and studied for their wound healing properties. The analysis of keratin-based hydrogel was done by Fourier Transform Infra-red Spectroscopy (FTIR) and X-ray diffraction (XRD) analysis.

Results: Keratinocytes containing keratin travel from the wound border to initiate the process of healing. The characteristics of keratin-based hydrogel derived from chicken feather made it an effective wound care therapeutic product. X-ray diffraction (XRD) analysis showed the crystallinity index in between 30-50% of the hydrogen.

Conclusion: The test for swelling and solubility were carried out on the hydrogen to determine the solid content and water absorbance capacity. Overall, this product is safe to use as an effective wound healing product with appropriate properties. 


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Mishra RK, Banthia AK, Majeed ABA. Pectin based formulations for biomedical applications: a review. Asian J Pharm Clin Res 2012;5:1-7.

Maziad NA, El-hamouly S, Zied E, El-Kelani TA, Nasef NR. Radiation preparation of smart hydrogel has antimicrobial properties for controlled release of ciprofloxacin in drug delivery systems. Asian J Pharm Clin Res 2015;8:193-200.

Sharma S, Gupta A. Sustainable management of keratin waste biomass: applications and future perspectives. Braz Arch Biol Technol 2016;59.

Nagam SP, Jyothi AN, Poojitha J, Aruna S, Nadendla RR. A comprehensive review on hydrogels. Int J Chem Pharm Res 2016;8:19-23.

Moll R, Divo M, Langbein L. The human keratins: biology and pathology. Histochem Cell Biol 2008;129:705-33.

Baranoski S, Ayello EA. Wound care essentials: practice principles: Lippincott Williams and Wilkins; 2008.

Kerstein MD. The scientific basis of healing. Adv Skin Wound Care 1997;10:30-6.

Katoh K, Shibayama M, Tanabe T, Yamauchi K. Preparation and properties of keratin–poly (vinyl alcohol) blend fiber. J Appl Polym Sci 2004;91:756-62.

Barone JR. Lignocellulosic fiber-reinforced keratin polymer composites. J Polym Environ 2009;17:143-51.

Wang T, Zhu XK, Xue XT, Wu DY. Hydrogel sheets of chitosan, honey and gelatin as burn wound dressings. Carbohydr Polym 2012;88:75-83.

Åženel S, McClure SJ. Potential applications of chitosan in veterinary medicine. Adv Drug Delivery Rev 2004;56:1467-80.

Molan PC. The role of honey in the management of wounds. J Wound Care 1999;8:415-8.

Viswanathan V, Kesavan R, Kavitha K, Kumpatla S. A pilot study on the effects of a polyherbal formulation cream on diabetic foot ulcers. Indian J Med Res 2011;134:168.

Gupta A, Perumal R. Process for extracting keratin. Google Patents US 20120130048 A1; 2013.

Gupta A, Kamarudin NB, Kee CYG, Yunus RBM. Extraction of keratin protein from the chicken feather. J Chem Chem Eng 2012;6:732.

Katayama T, Nakauma M, Todoriki S, Phillips GO, Tada M. Radiation-induced polymerization of gum arabic (Acacia senegal) in aqueous solution. Food Hydrocoll 2006;20:983-9.

Aikawa K, Matsumoto K, Uda H, Tanaka S, Shimamura H, Aramaki Y, et al. Hydrogel formation of the pH response polymer polyvinyl acetal diethylamino acetate (AEA). Int J Pharm 1998;167:97-104.

Aouada FA, de Moura MR, Fernandes PR, Rubira AF, Muniz EC. Optical and morphological characterization of polyacrylamide hydrogel and liquid crystal systems. Eur Polym J 2005;41:2134-41.

El Fray M, Pilaszkiewicz A, Swieszkowski W, Kurzydlowski KJ. Morphological assessment of chemically modified restructured poly (vinyl alcohol) hydrogel. Eur Polym J 2007;43:2035-40.

Pourjavadi A, Kurdtabar M. Collagen-based highly porous hydrogel without any porogen: Synthesis and characteristics. Eur Polym J 2007;43:877-89.

Mansur HS, Oréfice RL, Mansur AA. Characterization of poly (vinyl alcohol)/poly (ethylene glycol) hydrogels and PVA-derived hybrids by small-angle X-ray scattering and FTIR spectroscopy. Polymer 2004;45:7193-202.

Torres R, Usall J, Teixido N, Abadias M, Vinas I. Liquid formulation of the biocontrol agent Candida sake by modifying water activity or adding protectants. J Appl Microbiol 2003;94:330-9.

Hench LL, Jones JR. Biomaterials, artificial organs and tissue engineering. Woodhead Publishing Limited and CRC Press LLC: Cambridge, England and Boca Raton, FL, USA; 2005.

Pal K, Banthia A, Majumdar D. Polymeric hydrogels: characterization and biomedical applications. Des Monomers Polym 2009;12:197-220.

Parsons D, Bowler PG, Myles V, Jones S. Silver antimicrobial dressings in wound management: a comparison of antibacterial, physical, and chemical characteristics. Wounds 2005;17:222-32.

Molan P. Establishing honey as a recognised medicine. J Am Apitherapy Soc 2000;7:7-9.

Mao N, Russell S. Nonwoven wound dressings. Textile Progress 2004;36:1-57.

Lozinsky V, Solodova E, Zubov A, Simenel I. Study of cryo structuration of polymer systems. XI. The formation of PVA cryogels by freezing–thawing the polymer aqueous solutions containing additives of some polyols. J Appl Polym Sci 1995;58:171-7.

Kokabi M, Sirousazar M, Hassan ZM. PVA–clay nanocomposite hydrogels for wound dressing. Eur Polym J 2007;43:773-81.

Singh A, Hosseini M, Hariprasad SM. Polyethylene glycol hydrogel polymer sealant for the closure of sutureless sclerotomies: a histologic study. Am J Ophthalmol 2010;150:346-51. e2.

Martínez-Hernández AL, Velasco-Santos C. Keratin fibres from chicken feathers: structure and advances in polymer composites. Keratin: Structure, Properties and Applications; 2012. p. 149-211.

Mohd Zohdi R, Bakar A, Zakaria MZ, Mohamed Mustapha N, Yusof N, Abdullah NH. The effect of topical application of Malaysian honey on burn wound healing. J Vet Med 2004;16:47-50.

Yoldas BE. Alumina gels that form porous transparent Al2O3. J Mater Sci 1975;10:1856-60.

Sahlin JJ, Peppas NA. Near-field FTIR imaging: a technique for enhancing spatial resolution in FTIR microscopy. J Appl Polym Sci 1997;63:103-10.

Roberts MJ, Bentley MD, Harris JM. Chemistry for peptide and protein PEGylation. Adv Drug Delivery Rev 2012;64:116-27.

Singh TJ, Bhat SV. Morphology and conductivity studies of a new solid polymer electrolyte: (PEG) xLiClO4. Bull Mater Sci 2003;26:707-14.

Coates J. In: Meyers RA. editor. Encyclopaedia of analytical chemistry. Chichester: Wiley; 2000. p. 10815–37.

Rouse JG, Van Dyke ME. A review of keratin-based biomaterials for biomedical applications. Materials 2010;3:999-1014.

Ding F, Shi X, Li X, Cai J, Duan B, Du Y. Homogeneous synthesis and characterization of quaternized chitin in NaOH/urea aqueous solution. Carbohydr Polym 2012;87:422-6.

Peppas NA. Hydrogels in medicine and pharmacy: properties and applications: CRC PressI Llc; 1987.

Berghmans H, Stoks W. Thermoreversible gelation of vinyl polymers. In: Kleintjens LA, Lemstra PJ. editors. Integration of fundamental polymer science and technology. Dordrecht: Springer Netherlands; 1986. p. 218-29.

Boateng JS, Matthews KH, Stevens HN, Eccleston GM. Wound healing dressings and drug delivery systems: a review. J Pharm Sci 2008;97:2892-923.



How to Cite

Kumaran, P., A. Gupta, and S. Sharma. “SYNTHESIS OF WOUND-HEALING KERATIN HYDROGELS USING CHICKEN FEATHERS PROTEINS AND ITS PROPERTIES”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 9, no. 2, Feb. 2017, pp. 171-8, doi:10.22159/ijpps.2017v9i2.15620.



Original Article(s)