Jayanudin ., Rochmadi .


Objective: This research aims to determine the effect of the spray drying condition against encapsulation efficiency and characterization microcapsules of red ginger oleoresin.

Methods: Preparation of encapsulation begun with the formation of emulsions by mixing red ginger oleoresin with chitosan solution which was dissolved with acetic acid 2% (v/v). The weight ratio of chitosan with red ginger oleoresin was 1: 1, 2: 1 and 3: 1 and then stirred using a homogenizer while added 2 ml tween 80 for 10 min. The size of emulsion droplet was measured using nanoparticle analyzer (NPA). The emulsion is formed and then inserted into the feed tank of a spray dryer. Inlet temperature of the spray dryer used in the 180 °C, 190 °C and 200 °C; and the spray dryer outlet temperature was 85 °C, feed rate at 2 L/h. The microcapsules formed were then analyzed encapsulation efficiency and characterization using scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (FTIR).

Results: Based on the research that has been done, the smallest effective diameter of the emulsion droplets was 216.4±1.5 nm and the largest was 2109.2±46.1 nm. The value of encapsulation efficiency ranged between 83.33±0.42%-99.15±0.02%. Increasing the weight ratio of chitosan with red ginger oleoresin and increase the spray drying inlet temperature, the encapsulation efficiency is also increased. The highest encapsulation efficiency was 99.15±0.02% occurred at 200 °C of spray drying inlet temperature and the weight ratio of chitosan with red ginger oleoresin of 3:1. Morphology analysis of the surface of microcapsules using scanning electron microscope (SEM) showed that the inlet temperature of 200 °C was obtained microcapsules with smooth surfaces. The Fourier transforms infrared spectroscopy (FTIR) analysis results indicating the absence of new compounds is formed.

Conclusion: This research indicates that the spray drying conditions affecting the encapsulation efficiency and morphological characteristics of the red ginger oleoresin microcapsules.


Chitosan, Encapsulation efficiency, Red ginger oleoresin, Spray drying, Surface oil, Total oil

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Ding SH, An KJ, Zhao CP, Li Y, Guo YH, Wang ZF. Effect of drying methods on volatiles of Chinese ginger (Zingiber officinale roscoe). Food Bioprod Process 2012;90:515-24.

Oboh G, Ayodele JA, Adedayo OA. Antioxidant and inhibitory effect of red ginger (Zingiber officinale var. rubra) and white ginger (Zingiber officinale roscoe) on Fe2+induced lipid peroxidation in rat brain in vitro. Exp Toxicol Pathol 2012;64:31–6.

Zick SM, Djuric Z, Ruffin MT, Litzinger AJ, Normolle DP, Alrawi S, et al. Pharmacokinetics of 6-gingerol, 8-gingerol, 10-gingerol, and 6-shogaol and conjugate me¬tabolites in healthy human subjects. Cancer Epidemiol Biomarkers Prev 2008;17:1930-6.

Rahmani AH, Al Shabrmi FM, Aly SM. Active ingredients of ginger as potential candidates in the prevention and treatment of diseases via modulation of biological activities. Int J Physiol Pathophysiol Pharmacol 2014;6:125-36.

Hasan HA, Rasheed Raauf AM, Abd Razik BM, Rasool Hassan BA. Composition and antimicrobial activity of the crude extracts isolated from Zingiber officinale by different solvents. Pharm Anat Acta 2012;3:1-5.

Reineccius G. Source book of flavor. 2nd ed. Netherlands: Springer Science+Business Media B.V; 1994. p. 252.

Zachariah TJ. Ginger. In: Parthasarathy VA, Chempakam B, Zachariah TJ. Chemistry of spices. 1st ed. London: CAB International; 2008. p. 70-96.

El-Ghorab AH, Nauman M, Anjum FM, Hussain S, Nadeem MA. Comparative study on chemical composition and antioxidant activity of ginger (Zingiber officinale) and Cumin (Cuminum cyminum). J Agric Food Chem 2010;58:8231-7.

Vernin G, Parkanyi C. Chemistry of ginger. In: Ravindran PN, Nirmal Babu K. Ginger. The genus Zingiber, Boca Raton. Florida: CRC Press; 2004. p. 87-180.

Singh G, Kapoor IPS, Singh P, Heluani CS, Lampasona MP, Cesar ANC. Chemistry antioxidant and antimicrobial investigations on essential oil and oleoresins of Zingiber officinale. Food Chem Toxicol 2008;46:3295–302.

Saha A, Blando J, Silver E, Beltran L, Sessler J, Di Giovanni J. 6-Shogaol from dried ginger inhibits growth of prostate cancer cells both in vitro and in vivo through inhibition of STAT3 and NF-kB signaling. Cancer Prev Res 2013;7:627-38.

Tan BS, Kang O, Mai CW, Tiong KH, Alan ASB, Pichika MR, et al. 6-Shogaol inhibits breast and colon cancer cell proliferation through activation of peroxisomal proliferator activated receptor γ (PPARγ). Cancer Lett 2013;336:127-39.

Lin CB, Lin CC, Tsay GJ. 6-Gingerol inhibits growth of colon cancer cell LoVo via induction of G2/M arrest. J Evidence-Based Complementary Altern Med 2012;1-7. http://dx.doi.org/ 10.1155/2012/326096

Govindarajan VS. Ginger-chemistry, technology, and quality evaluation: part I. Crit Rev Food Sci Nutr 1982;17:1-96.

Shaikh J, Bhosale R, Singhal R. Microencapsulation of black pepper oleoresin. Food Chem 2006;94:105-10.

Purseglove JW. Spices. Vol. II, New York: Longman Inc; 1981.

Vaidya S, Bhosale R, Singhal RS. Microencapsulation of cinnamon oleoresin by spray drying using different wall materials. Drying Technol 2006;24:983-92.

Dubey R, Shami TC, Rao KUB. Microencapsulation technology and applications. Def Sci J 2009;59:82-95.

Bansode SS, Banarjee SK, Gaikwad SL, Jadhav R, Thorat RM. Microencapsulation: a review. Int J Pharm Sci Rev Res 2010;1:38-43.

Gouin S. Microencapsulation: industrial appraisal of existing technologies and trends. Trend Food Sci Tech 2004;15:330–47.

Calvo P, Hernandez T, Lozano M, Gonzalez-Gomez D. Microencapsulation of extra-virgin oil by spray drying: Influence of wall material and olive quality. Eur J Lipid Sci Tech 2010;112:852-8.

Kumar A, Sharma PK, Banik A. Microencapsulation as a novel drug delivery system. Int Pharm Sci 2011;1:1-7.

Agnihotri N, Mishra R, Goda C, Arora M. Microencapsulation–a novel approach in drug delivery: a review. Indo Global J Pharm Sci 2012;2:1-20.

Hejazi R, Amiji M. Chitosan-based gastrointestinal delivery systems. J Controlled Release 2003;89:151-65.

Pavanveena C, Kavitha K, Anil Kumar SN. Formulation and evaluation of trimetazidine hydrochloride loaded chitosan microsphere. Int J Appl Pharm 2010;2:11-4.

Dziezak JD. Microencapsulation and encapsulated ingredients. Food Tech 1988;42:136-48.

Gharsallaoui A, Roudaut G, Chambin O, Voilley A, Saurel R. Applications of spray-drying in microencapsulation of food ingredients: an overview. Food Res Int 2007;40:1107–21.

Shahidi F, Han XQ. Encapsulation of food ingredients. Crit Rev Food Sci Nutr 1993;33:501-47.

Tonon RV, Grosso CRF, Hubinger MD. Influence of emulsion composition and inlet air temperature on the micro-encapsulation of flaxseed oil by spray drying. Food Res Int 2011;44:282-9.

Şahin-Nadeem H, Dinçer C, Torun M, Topuz A, Özdemir F. Influence of inlet air temperature and carrier material on the production of instant soluble sage (Salvia fruticosa Miller) by spray drying. LWT Food Sci Technol 2013;52:31-8.

Harimurti N, Nhestricia N, Subardjo SY, Yuliani S. Effect of oleoresin concentration and composition of encapsulating materials on properties of the microencapsulated ginger oleoresin using spray drying method. Indonesian J Agric 2011;4:33-9.

Tan LH, Chan LW, Heng PW. Effect of oil loading on microspheres produced by spray-drying. J Microencapsul 2005;22:253-9.

Aghbashlo M, Mobli H, Madadlou A, Rafiee S. Influence of wall material and inlet drying air temperature on the microencapsulation of fish oil by spray drying. Food Bioprocess Technol 2013;6:1561-9.

Eid AM, Saringat HB, Arafat OM. The effect of surfactant blends on the production of self-emulsifying system. Int J Pharma Frontier Res 2012;2:21-31.

Ruo L. Chitosan particles for the controlled release of proteins. Doctoral dissertation. Department of Mechanics. Politecnico di Torino. Italy; 2012.

Bhardwaj SB, Shukla AJ, Collins CC. Effect of varying drug loading on particle size distribution and drug release kinetics of verapamil hydrochloride microspheres prepared with cellulose esters. J Microencapsul 1995;12:71-81.

Joshi HC, Pandey IP, Kumar A, Garg N. A study of various factors determining the stability of molecules. Adv Pure Appl Chem 2012;1:7-11.

Pourashouri P, Shabanpour B, Razavi SH, Jafari SM, Shabani A, Aubourg SP. Impact of wall materials on physicochemical properties of microencapsulated fish oil by spray drying. Food Bioprocess Tech 2014;7:2354-65.

McNamee BF, O’Riordan ED, O’Sullivan M. Emulsification and microencapsulation properties of gum arabic. J Agric Food Chem 1998;46:4551–5.

Bhandari BR, Dumoulin ED, Richard HMJ, Noleau I, Lebert AM. Flavor encapsulation by spray drying-application to citral and linalyl acetate. J Food Sci 1992;57:217–21.

Jafari SM, Assadpoor E, He Y, Bhandari B. Encapsulation efficiency of food flavors and oils during spray drying. Drying Technol 2008;26:816-35.

Ganesh S, Kumar DS, Kumar BS, Abhilash R, Bharadwaj PS, Prudhviraj KVS, et al. Controlled release formulation and evaluation of idarubicin microsphere using biodegradable hydrophilic and hydrophobic polymer mixtures. Asian J Pharm Clin Res 2010;3:179-82.

Minemoto Y, Hakamata K, Adachi S, Matsuno R. Oxidation of linoleic acid encapsulated with gum arabic or maltodextrin by spray-drying. J Microencapsul 2002;19:181-9.

Maa YF, Constantino HR, Nguyen PA, Hsu CC. The effect of operating and formulation variables on the morphology of spray-dried protein particles. Pharm Dev Technol 1997; 2:213-23.

Mezhericher M, Levy A, Borde I. Spray drying modelling based on advanced droplet drying kinetics. Chem Eng Proc: Process Intensif 2010;49:1205–13.

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Chitosan, Encapsulation efficiency, Red ginger oleoresin, Spray drying, Surface oil, Total oil





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International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 8, 2017 Page: 29-34

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Authors & Affiliations

Jayanudin .
Chemical Engineering Department, Sultan Ageng Tirtayasa University indonesia

Rochmadi .
Chemical Engineering Department, Sultan Ageng Tirtayasa University indonesia

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