SCREENING OF ANTIOXIDANT ACTIVITY AND TOTAL PHENOLIC CONTENT IN RAPHANUS SATIVUS POD
Keywords:Raphanus sativus L Var caudatus Alef, DPPH, FRAP, Total phenolic content
Objective: The aim of this study was to evaluate the antioxidant activity and total phenolic content of Raphanus sativus L. Var. caudatus Alef pod extracts.
Methods: In this study, the extract of R. sativus pod was prepared in three different types of solvent. The antioxidant activitiy of R. sativus extract was determined using a spectrophotometric technique, based on a free radical diphenyl-picryhydrazyl scavenging assay (DPPH assay) and a ferric reducing antioxidant power assay (FRAP assay). Total phenolic content was also observed using the spectrophotometric technique.
Results: The result showed that the antioxidant activity which was expressed by IC50 values varied from 1,365 to 4,371 mg/ml and 312 to 6,478 mg/ml, based on DPPH assay and FRAP assay, respectively. Total phenolic content was also evaluated and calculated as gallic acid equivalents which ranged from 0.26 to 34.60 mg gallic acid per 100 g fresh sample.
Conclusion: It was suggested that hexane extract of R. sativus pod contained the highest amount of phenolic compounds in comparison with those of dichloromethane part and ethanol part. The result from FRAP assay was positively correlated to total phenolic content which the highest antioxidant value belongs to the hexane extract of R. sativus pod. It was concluded that R. sativus pod contained phenolic compounds which showed mild antioxidant activity.
Shahidi F. Antioxidants in food and food antioxidants. J Nahrung 2000;44:158-63.
Halliwell B. Reactive oxygen species in living systems:source, biochemistry, and role in human disease. Am J Med 1991;91:14-22.
Loliger J. The use of antioxidants in food. In:Aruoma OI, Halliwell B, editors. Free radicals and food additives. London:Taylor and Francis;1991. p. 129-50.
Osawa T, Uritani I, Garcia VV, Mendoza EM, editors. Novel natural antioxidants for utilization in food and biological systems. In:Postharvest biochemistry of plant food-materials in the tropics. Tokyo:Japan Scientific Societies Press;1994. p. 241-51.
Pietta PG. Flavonoids in medicinal plants. In:Rice-Evans CA, Packer L, editors. Flavonoids in health and disease. New York:Dekker;1998. p. 61â€“110.
Songsak T, Lockwood GB. Glucosinolates of seven medicinal plants from Thailand. J Fitoterapia 2002;73:209-16.
Pocasap P, Weerapreeyakul N, Barusrux S. Cancer preventive effect of Thai rat-tailed radish (Raphanus sativus L. var caudatus Alef). J Func Foods 2013;5:1372-81.
Holst B, Williamson G. A critical review of the bioavailability of glucosinolates and related compounds. J Nat Prod Rep 2004;21:425-47.
Settharaksa S, Madaka F, Sueree L, Chankana N, Chakree K, Charoenchai L. Cytotoxic activity and antioxidant potentials of cold pressed rice bran oil. Int J Pharm Tech Res 2014;6:686-91.
Oliveira AMF, Pinheiro LS, Pereira CKS, Matias WN, Gomes RA, Chaves OS, et al. Total phenolic content and antioxidant activity of some Malvaceae family species. J Antioxidants 2012;1:33-43.
Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. J LWT-Food Sci Technol 1995;28:25-30.
Benzie IFF, Strian JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant powerâ€:the FRAP assay. J Anal Biochem 1996;239:70-6.
Slinkard K, Singleton VL. Total phenol analysis: Automation and comparison with manual methods. Am J Enol Vitic 1997;28:49-55.
Hamed A, Soltan M, Fry J, Hammouda F, Zaki A. Antioxidant and cytoprotective properties of three Egyptian Cyperus species using cell-free and cell based assays. J Pharm Crops 2012;3:88-93.