VALIDATED SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF VINCRISTINE AND VINBLASTINE
Objective: A simple, sensitive, precise, reproducible and validated UV spectrophotometric methods have been developed for the determination of vincristine (VCR) and vinblastine (VLB) in the pure and dosage forms.
Methods: The method was founded on the simple solubility of VCR and VLB in purified water, and their characteristic maximum absorption Î» (max) at 295 nm and Î» (max) at 268 nm for VCR and VLB respectively in the UV regions. The nature of obedience, to the Bouguer-Lambert-Beer's law by the VCR and VLB in the range of concentration 5-50 Âµg/ml was employed to this method.
Results: Accuracy and reproducibility of the proposed method were statistically validated by recovery studies. The accuracy of the method for the VCR and VLB was ~ 100.4 % and ~ 100.32 % respectively with good reproducibility. The analytical curves were linear over a wide concentration range (5-50 Âµg/ml), with a correlation coefficient (r)-0.9998, and 0.9999 for VCR and VLB in that order. The method was showed sufficient precision, with a relative standard deviation (RSD) less than 1%.
Conclusion: The method was validated in accordance with Russian general pharmacopoeia article (RGPA) 42-0113-09 and ICH guidelines. Validated method can easily apply for fast, precise and reliable rapid assessment of drug forms and pure substances in the laboratory.
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