NORMAL VASCULAR REACTIVITY IS RESTORED BY APIGENIN IN DIABETIC RATS


Hany M. El-bassossy, Nora Desoky, Abdulrahman M Alahdal, Ahmed Fahmy

Abstract


Objective: Diabetes is a disease whose complications have serious implications for the health of sufferers; one of the most serious such complications is the deterioration of vascular reactivity. Apigenin is a natural flavonoid with PKC inhibiting and antioxidant properties. In this study, the impact of apigenin on vascular reactivity deterioration was investigated.

Methods: Insulin resistance (IR) and insulin deficiency (ID) were induced by fructose and streptozotocin respectively. The isolated aortae vasoconstriction response to phenylephrine (PE) and potassium chloride (KCl) in addition to the vasodilation response to acetylcholine (ACh) and sodium nitroprusside (SNP) were tested.

Results: IR and ID were associated with significantly exaggerated vasoconstriction to KCl and PE while significantly impaired vasodilation to ACh. Response to SNP was not significantly affected by both IR and ID. In vitro incubation with apigenin (7 7µM) for 20 min restored normal responses to PE, KCl and ACh in aortae isolated from insulin-resistant or insulin-deficient rats. Incubation for one hour with the PKC stimulant, phorbol 12-myristate 13-acetate (PMA, 800 nM) resulted in aortic impairment similar to that seen in aortae isolated from IR and ID animals. Incubation with both apigenin prevented PMA-induced exaggerated vasoconstriction response to both PE and KCl.

Conclusion: Apigenin alleviates vascular exaggerated vasoconstriction and impaired dilation associated with diabetes or PKC activated.


Keywords


Apigenin, Diabetes, PMA, Isolated aorta, Vascular reactivity

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About this article

Title

NORMAL VASCULAR REACTIVITY IS RESTORED BY APIGENIN IN DIABETIC RATS

Keywords

Apigenin, Diabetes, PMA, Isolated aorta, Vascular reactivity

DOI

10.22159/ijpps.2018v10i1.16621

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 10, Issue 1, 2018 Page: 27-31

Online ISSN

0975-1491

Statistics

68 Views | 76 Downloads

Authors & Affiliations

Hany M. El-bassossy
Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Kingdom of Saudi Arabia, Department of Pharmacology, Faculty of Pharmacy, Zagazig University, Egypt,
Saudi Arabia

Nora Desoky
Department of Pharmacology, Faculty of Pharmacy, Zagazig University, Egypt,
Egypt

Abdulrahman M Alahdal
Faculty of Pharmacy, Department of Clinical Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
Saudi Arabia

Ahmed Fahmy
Department of Pharmacology, Faculty of Pharmacy, Zagazig University, Egypt
Egypt


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