GROWTH CHARACTERIZATION OF CALCIUM HYDROGEN PHOSPHATE DIHYDRATE CRYSTALS INFLUENCED BY COSTUS IGNEUS AQUEOUS EXTRACT

  • Thanikasalam Yuvarani Department of Biochemistry and PRIST University Vallam, Tamilnadu India 613403
  • Kesavan Manjula 3Bio Techno Solutions Training and Research Institute, No:11, Highways Colony Subramaniapuram, Mary Tiffany Play School upstairs, Trichy-620020
  • Perumal Ananda Gopu 2Center for Advanced Computing and Bioinformatics PRIST University Vallam, Tamilnadu India 613403

Abstract

Objective: To investigate the inhibitory effect of aqueous extract of leaves, stems and rhizome of Costus igneus on the growth of calcium hydrogen phosphate dihydrate (CHPD) crystals.

Methods: Calcium hydrogen phosphate dihydrate (CHPD) crystals were grown by the single diffusion gel growth technique and the inhibitory effect of aqueous extracts of leaves, stems and rhizome of Costus igneus on the growth of CHPD crystals has been studied. The grown crystals were characterised by Fourier Transform Infrared Spectroscopy (FTIR), Powder X-Ray diffraction (XRD) for further confirmations.

Results: With an increase in the concentration of aqueous extract of Costus igneus, the weight of the formed crystals were gradually reduced from 2.03 g to 0.06 g (leaves), 0.05 g (rhizome), 0.03 g (stem) for the CHPD crystals, respectively. The crystals harvested from the CHPD were characterised by Fourier Transform Infrared Spectroscopy (FTIR) to confirm the functional groups, and Powder X-Ray Diffraction technique (XRD) analyses to confirm the crystalline phases of the CHPD and hydroxyapatite (HAP) crystals. Results obtained indicated that Costus igneus (leaves, stems and rhizome) has the potential to inhibit the formation of calcium hydrogen phosphate dihydrate crystals.

Conclusion: This study confirms that using an aqueous extract of stem and rhizome of Costus igneus can promote the formation of hydroxyapatite (HAP) crystals and reduce the nucleation rate of CHPD crystals, a major component of calcium urinary stone.

Keywords: Calcium phosphate, Hydroxyapatite, Costus igneus, Fourier Transform Infrared Spectroscopy (FTIR), Powder X-Ray diffraction (XRD)

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Yuvarani, T., K. Manjula, and P. A. Gopu. “GROWTH CHARACTERIZATION OF CALCIUM HYDROGEN PHOSPHATE DIHYDRATE CRYSTALS INFLUENCED BY COSTUS IGNEUS AQUEOUS EXTRACT”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 9, no. 5, May 2017, pp. 173-8, doi:10.22159/ijpps.2017v9i5.16838.
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