GROWTH CHARACTERIZATION OF CALCIUM HYDROGEN PHOSPHATE DIHYDRATE CRYSTALS INFLUENCED BY COSTUS IGNEUS AQUEOUS EXTRACT
Objective: To investigate the inhibitory effect of aqueous extract of leaves, stems and rhizome of Costus igneus on the growth of calcium hydrogen phosphate dihydrate (CHPD) crystals.
Methods: Calcium hydrogen phosphate dihydrate (CHPD) crystals were grown by the single diffusion gel growth technique and the inhibitory effect of aqueous extracts of leaves, stems and rhizome of Costus igneus on the growth of CHPD crystals has been studied. The grown crystals were characterised by Fourier Transform Infrared Spectroscopy (FTIR), Powder X-Ray diffraction (XRD) for further confirmations.
Results: With an increase in the concentration of aqueous extract of Costus igneus, the weight of the formed crystals were gradually reduced from 2.03 g to 0.06 g (leaves), 0.05 g (rhizome), 0.03 g (stem) for the CHPD crystals, respectively. The crystals harvested from the CHPD were characterised by Fourier Transform Infrared Spectroscopy (FTIR) to confirm the functional groups, and Powder X-Ray Diffraction technique (XRD) analyses to confirm the crystalline phases of the CHPD and hydroxyapatite (HAP) crystals. Results obtained indicated that Costus igneus (leaves, stems and rhizome) has the potential to inhibit the formation of calcium hydrogen phosphate dihydrate crystals.
Conclusion: This study confirms that using an aqueous extract of stem and rhizome of Costus igneus can promote the formation of hydroxyapatite (HAP) crystals and reduce the nucleation rate of CHPD crystals, a major component of calcium urinary stone.
2. Tiwari A, Soni V, Londhe V, Brandarkar A, Bandawanw D, Nipate S. An overview on potent indigenous herbs for urinary tract infirmity: urolithiasis. Asian J Pharm Clin Res 2012;5 Suppl 1:7-12.
3. Beghalia M, Ghalem S, Allali H, Belouatek A, Marouf A. Inhibition of calcium oxalate monohydrate crystal growth using Algerian medicinal plants. J Med Plants Res 2008;2 Suppl 3:66-70.
4. Aggarwal S, Tandon CD, Forouzanveh M, Simgla SK, Kiran R, Jethi RK. Role of biomolecules from the human renal stone matrix on COM crystal growth. Mol Cell Biochem 2000; 210:109â€“19.
5. Daudon M, Bader CA, Jungers P. Urinary calculi: a review of classification methods and correlations with etiology. Scanning Microsc 1993;7 Suppl 3:1081-106.
6. Joshi VS, Parekh BB, Joshi MJ, Vaidya AB. Herbal extract of Tribulus terrestris and Bergenia ligulata inhibit growth of calcium oxalate monohydrate crystals in vitro. J Cryst Growth 2005;275:1403-8.
7. El-Shall H, Jeon JH, Abdel-Aal EA, Khan S, Gower L, Rabinovich Y. A study of primary nucleation of calcium oxalate monohydrate: II. Effect of urinary species. Cryst Res Technol 2004;39 Suppl 7:577-85.
8. Bouropoulos C, Vagenas N, Klepetsanis P, Stavropoulos N, Bouropoulos N. Growth of calcium oxalate monohydrate on uric acid crystals at sustained supersaturation. Cryst Res Technol 2004;39 Suppl 8:699-704.
9. Anjian X, Yuhua S, Dong M, Fangzhi H, Lingguang Q, Shikuo L Chen L. Growth of calcium oxalate crystals induced by complex films containing biomolecules. Cryst Res Technol 2007;42 Suppl 7:667-72.
10. Monje PV, Baran EJ. Characterization of calcium oxalate generated as biominerals in cacti. Plant Physiol 2002;128 Suppl 2:707-13.
11. Valarmathi D, Abraham L, Gunasekaran S. Growth of calcium oxalate monohydrate crystal by gel method and its spectroscopic analysis. Indian J Pure Appl Phys 2010;48 Suppl 8:36-8.
12. Yongtai Z, Jiansheng T, Nianping F, Xiangdong H. Crystal growth of calcium oxalate induced by the extracts of Semen plantaginis and Folium pyrrosiae. Cryst Res Technol 2008;43:931-4.
13. Sheng X, Ward MD, Wesson JA. Crystal surface adhesion explains the pathological activity of calcium oxalate hydrates in kidney stone formation. J Am Soc Nephrol 2005;16:1904-8.
14. Rajendran K, Dale keefe C. Growth and characterization of calcium hydrogen phosphate dihydrate crystals from single diffusion gel technique. Cryst Res Technol 2010;45:939-45.
15. Doddametikurke RB, Chandra SB, Anthony J, Browning Cartledge J. The role of urinary kidney stone inhibitors and promoters in the pathogenesis of calcium containing renal stones. Eur Urol 2007;5:126-36.
16. Madhurambal G, Subha R, Mojumdar SC. Crystallization and thermal characterization of calcium hydrogen phosphate dihydrate crystals. J Therm Anal Calorim 2009;96:73-6.
17. Prasobh GR, Revikumar KG. Use of musa AAB in kidney stone treatment and other diseases. Asian J Pharm Clin Res 2011;4 Suppl 3:117-8.
18. Fouad A, Yamina S, Mostapha M, Mohammed A, Brahim H. Effect of aqueous extract from Herniaria hirsute L. on experimentally nephrolithiasic rats. J Ethnopharmacol 2004;95 Suppl 1:87-93.
19. Bensatal A, Ouahrani MR. Inhibition of crystallization of calcium oxalate by the extraction of Tamarix gallica L. Urol Res 2008;36 Suppl 6:283-7.
20. Devi VD, Urooj A. Nutrient profile and antioxidant components of Costus speciosus Sm. and Costus igneus Nak. Indian J Nat Prod Resour 2010;1:116-8.
21. Devi VD, Urooj A. Hypoglycemic potential of Morus indica L and Costus igneus Nakâ€“A preliminary study. Indian J Exp Biol 2008;46 Suppl 8:614-6.
22. Araiyo viel T, Diogo Domingos C, Paula da Silva Monteiro A, Teresa Riggio Lima-Landman M, Jose lopa A, Souccar C. Evaluation of the antiurolithiatic activity of the extract of Costus spiralis roscoe in rats. J Ethnopharmacol 1999;66:193-8.
23. Bhat V, Naveen A, Akshay K, Sikarwar Mukesh S, Patil MB. Antidiabetic activity of insulin plant (Costus igneus) leaf extract in diabetic rats, J Pharm Res 2010;3 Suppl 3:608-11.
24. Jose B, Reddy LJ. Analysis of the essential oils of the stems, leaves and rhizomes of the medicinal plant Costus pictus from Southern India. Int J Pharm Pharm Sci 2010;2 Suppl 2:100-1.
25. Pazhanichamy K, Aiswarya Devi S, Jeba SC, Priya G, Yogha G, Manjula K, et al. The efficacy of Costus igneus rhizome on carbohydrate metabolic, hepatoproductive and antioxidative enzymes in streptozotocin-induced diabetic rats. J Health Sci 2011;57 Suppl 1:37-46.
26. Joshi VS, Parekh BB, Joshi MJ, Vaidya ADB. Inhibition of growth of urinary calcium hydrogen phosphate dihydrate crystals with aqueous extracts of Tribulus terrestris and Bergenia ligulata. Urol Res 2005;33:80-6.
27. Joseph KC, Joshi MJ. The study of different parameters affecting liesegang ring formation during the growth of calcium hydrogen phosphate dihydrate crystals. Indian J Phys 2002;76A:159.
28. Henisch HK, Garcia-Ruiz JM. Crystal growth in gels and liesegang ring formation. J Cryst Growth 1986;75:195.
29. Chaudhary A, Singla SK, Tandon C. In vitro evaluation of Terminalia arjuna on calcium phosphate and calcium oxalate crystallization. Indian J Pharm Sci 2010;72 Suppl 3:340-5.
30. Joshi VS, Joshi MJ. Influence of inhibition of citric acid and lemon juice to the growth of calcium hydrogen phosphate dihydrate urinary crystals. Indian J Pure Appl Phys 2003;41:183-92.
31. Joseph KC, Parekh BB, Joshi MJ. Inhibition of growth of urinary type calcium hydrogen phosphate dihydrate crystals by tartaric acid and tamarind. Curr Sci 2005;88(8 Suppl 25):1232-8.
32. Joshi VS, Joshi MJ. FTIR spectroscopic, thermal and growth morphological studies of calcium hydrogen phosphate dihydrate crystals. Cryst Res Technol 2003;38:817-21.
33. Roop Kumar R, Wang M. Biomimetic deposition of hydroxyapatite on brushite single crystals grown by the gel technique. Mater Lett 2001;49:15-9.
34. Markovie M, Fowler BO, Tung MS. Preparation and comprehensive characterization of a calcium hydroxyapatite reference material. J Res Nat Inst Stand Technol 2004;109:553-68.