EVALUATION OF ANTIBACTERIAL POTENTIAL OF SILVER NANOPARTICLES (SNPs) PRODUCED USING RHIZOME EXTRACT OF HEDYCHIUM CORONARIUM J. KOENIG

  • Sinjumol Thomas The Rapinat Herbarium and Centre for Molecular Systematics, St. Joseph’s College (Autonomous), Tiruchirappalli, Tamilnadu-620002
  • S. John Britto The Rapinat Herbarium and Centre for Molecular Systematics, St. Joseph’s College (Autonomous), Tiruchirappalli, Tamilnadu, India 620002
  • Silvy Mathew The Rapinat Herbarium and Centre for Molecular Systematics, St. Joseph’s College (Autonomous), Tiruchirappalli, Tamilnadu, India 620002
  • Bince Mani Postgraduate and Research Department of Botany, St. Thomas College, Palai, Arunapuram P. O., Kottayam, Kerala, India 686574

Abstract

Objective: The bio-reduction of silver ions in solution into silver nanoparticles by rhizome extract of Hedychium coronarium as capping, reducing and stabilizing agent. Further, to evaluate the antibacterial activity of the phytosynthesized silver nanoparticles.

Methods: Aqueous extract of dried rhizome powder of Hedychium coronarium were used for the green synthesis of silver nanoparticles. The synthesized nanoparticle was characterized by UV-vis spectroscopy, Scanning Electron Microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared spectroscopy (FTIR) studies. Antibacterial activity of silver nanoparticles was carried out by disc diffusion method.

Results: The observation of the peak at 428 nm in the UV-vis spectrum for phytosynthesized silver nanoparticles reveals the reduction of silver metal ions into silver nanoparticles. The FTIR analysis was performed to identify the possible functional groups involved in the synthesis of silver nanoparticles. The SEM image shows that most of the phytosynthesized silver nanoparticles have spherical morphology. The average diameter of the particles was calculated from the XRD pattern and it was found to be 24 nm. Further, it was observed that silver nanoparticles have high antibacterial activity especially against gram negative organisms.

Conclusion: The rhizome extract of Hedychium coronarium could be a good candidate for the green synthesis of silver nanoparticles. The antibacterial property of silver nanoparticles is a beneficial application in the field of medical nanotechnology.

Keywords: Hedychium coronarium, Silver nanoparticles, Rhizome, SEM, FTIR.

Downloads

Download data is not yet available.

References

1. Nalwa HS. Hand book of nanostructured Biomaterials and their applications in Nanobiotechnology. Los Angeles: American scientific publishers;2005.
2. Prabhu N, Divya TR, Yamuna G. Synthesis of silver phyto nanoparticles and their antibacterial efficacy. Dig J Nanomater Bios 2010;5:185-9.
3. Dinesh S, Karthikeyan S, Arumugam P. Biosynthesis of silver nanoparticles from Glycyrrhiza glabra root extract. Arch Appl Sci Res 2012;4:178-87.
4. Willner IR, Baron R, Willner B. Growing metal nanoparticles by enzymes. Adv Mater 2006;18:1109–20.
5. Mishra B, Patel BB, Tiwari S. Colloidal nanocarriers: a review on formulation technology, types and applications toward targeted drug delivery. Nanomedicine 2010;6:9-24.
6. Baker C, Pradhan A, Pakstis L, Pochan DJ, Shah SI. Synthesis and antibacterial properties of silver nanoparticles. J Nanosci Nanotechnol 2005;5:244-9.
7. Thirumurgan A, Tomy NA, Jai Ganesh R, Gobikrishnan S. Biological reduction of silver nanoparticles using plant leaf extracts and its effect an increased antimicrobial activity against clinically isolated organism. Pharm Chem 2010;2:279-84.
8. Kiritikar KR, Basu BD. Indian Medicinal Plants. Allahabad: Lalit Mohan Basu; 1933.
9. Chopra RN, Chopra IC, Verma BS. Supplement to Glossary of Indian Medicinal Plants. New Delhi: Publication and Information Directorate;1969.
10. Itokawa H, Morita H, Takeya K, Motidome M. Diterpenes from rhizomes of Hedychium coronarium. Chem Pharm Bull 1988;36(7):2682–4.
11. Chimnoi N, Pisutiaroenpong S, Ngiwsara L, Dechtrirut D, Chokchaichamnankit D, Khunnawutmanotham N, et al. Labdane diterpenes from the rhizomes of Hedychium coronarium. Nat Prod Res 2008;22(14):1255–62.
12. Shankar SS, Rai A, Ankamwar B, Singh A, Ahmad A, Sastry M. Biological synthesis of triangular gold nanoprisms. Nature Materials 2004;3:482.
13. Jagtap UB, Bapat VA. Green synthesis of silver nanoparticles using Artocarpus heterophyllus Lam. seed extract and its antibacterial activity. Ind Crop Prod 2013;46:132–7.
14. Cruickshank R. Medical microbiology: a guide to diagnosis and control of infection. 11th ed. Edinburgh and London: E&S Livingston Ltd;1968.
15. Nabikhan A, Kandasamy K, Raj A, Alikunhi NM. Synthesis of antimicrobial silver nanoparticles by callus and leaf extracts from saltmarsh plants, Sesuvium portulacastrum L. Colloids Surf B Interface 2010;79:488-93.
16. Savithramma N, Rao ML, Rukmini K, Suvarnalatha devi P. Antimicrobial activity of Silver Nanoparticles synthesized by using Medicinal Plants. Int J Chem Tech Res 2011;3:1394-402.
17. Ahmad N, Sharma S, Singh VN, Shamsi SF, Fatma A, Mehta BR. Biosynthesis of silver nanoparticles from Desmodium trifolium: a novel approach towards weed utilization. Biotechnol Res Int 2011;1-8.
18. Lee HY, Park HK, Lee YM, Kim K, Park S. Silver nanoparticles and its antibacterial evaluation for biomedical applications. Chem Commun 2007;28:2885.
19. Pal S, Tak YK, Song JM. Does the antibacterial activity of silver nanoparticles depend on the shape of the Nanoparticle? A study of the gram-negative bacterium Escherichia coli. Appl Environ Microbiol 2007;73:1712-20.
Statistics
271 Views | 2500 Downloads
How to Cite
Thomas, S., S. J. Britto, S. Mathew, and B. Mani. “EVALUATION OF ANTIBACTERIAL POTENTIAL OF SILVER NANOPARTICLES (SNPs) PRODUCED USING RHIZOME EXTRACT OF HEDYCHIUM CORONARIUM J. KOENIG”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 10, 1, pp. 92-95, https://innovareacademics.in/journals/index.php/ijpps/article/view/1699.
Section
Original Article(s)