THE POSSIBILITY OF DEVELOPMENT OF ANTIBIOTIC RESISTANCE FROM MASSIVE AND UNCONTROLLED USE OF SULFONAMIDES IN MILK


Zana Ibraimi, Agim Shehi, Ardiana Murtezani, Fehim Haliti

Abstract


Objective: The aim of this study was to discover sulfa residues in milk and to determine the most appropriate time required for allowing the consumption of milk after drug’s use in dairy cattle.

Methods: 150 samples of raw milk were collected from 95% of dairy farms in Kosovo that were visited during years 2015-2016. At these farms, using a questionnaire, we have identified the treatment procedures of dairy cattle, the dose and the type of drug administered to them, as well as the duration of this administration. Then, ELISA screening method and High-Performance Liquid Chromatography with Fluorescence Detection (HPLC/FD) confirmatory method were used to analyse the collected samples.

Results: Out of 150 milk samples, 52% were given a combination of 400 mg sulfadiazine+80 mg trimethoprim, with a statistically significant difference compared to other sulfonamides p<0.01. During the examination by ELISA method, 8 of them (5%) had exceeded the limit of detection, while during the examination by HPLC/FD method, 4 of them (2.5%) had exceeded the maximum residue limit allowed. In the first 4 d after the treatment has ended, the level of sulfonamide residues was high 141.5%, which is as high as their initial dose, with statistically significant linear trend p<0.01.

Conclusion: This study demonstrates that in the collected raw milk there were sulfonamide residues, whose level has fallen following 4 d of treatment of the animal. It also shows the necessary time allowed for milk consumption. During this time milk must not be consumed in order to prevent the development of antibiotic resistance in the human population.


Keywords


Antibiotic resistance, Sulfonamides, Residues, Milk, ELISA, HPLC

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About this article

Title

THE POSSIBILITY OF DEVELOPMENT OF ANTIBIOTIC RESISTANCE FROM MASSIVE AND UNCONTROLLED USE OF SULFONAMIDES IN MILK

Keywords

Antibiotic resistance, Sulfonamides, Residues, Milk, ELISA, HPLC

DOI

10.22159/ijpps.2017v9i4.17298

Date

03-03-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 4, 2017 Page: 207-211

Online ISSN

0975-1491

Statistics

102 Views | 95 Downloads

Authors & Affiliations

Zana Ibraimi
University of Prishtina, Faculty of Medicine, Department of Pharmacy
Albania

Agim Shehi
University of Tirana, Faculty of Medicine
Albania

Ardiana Murtezani
University of Prishtina, Faculty of Medicine
Albania

Fehim Haliti
University of Pristina, Faculty of Medicine
Albania


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