EFFECT OF ETHANOLIC EXTRACT OF TINOSPORA CRISPA L FROM INDONESIA IN ALLOXAN INDUCED LIVER DAMAGE
Objective: The objective of this study was to evaluate the hepatoprotective effect of Tinospora crispa L. (Bratawali).
Methods: Twenty four male rats wistar strain were divided into four groups. The serum alanine amino transferase (ALT) and aspartate aminotransferase (AST) of rats were measured (day 0). Rats then were injected by alloxan monohydrate at doses of 120 mg/200 bw (g) intraperitoneally. Four days later, the serum ALT and AST of rats were measured (second measurement/day 4) and then were treated by extract appropriate their groups. Group 1 was treated by 2 ml of distilled water orally; group 2,3 and 4 were treated by 70% ethanolic extract of T. crispa L. (EETC) at dose of 100; 200 and 400 mg/200 bw (g)/day respectively orally. After 10 d treatment, serum ALT and AST were measured (third measurement/day 14). At the end of this treatment, all rats were killed for histopathologic examination of their liver. The histopathologic examination was performed to assess the number of pyknotic nuclei, karyorrhexis nuclei and karyolysis nuclei.
Results: The result of this study showed that the ethanolic extract of T. crispa L at dose of 100 and 200 mg/200gbw can reduce blood ALT and AST significantly (P<0.05). From the histopathological examination, it was found that the number of pyknotic nuclei, karyorrhexis nuclei and karyolysis nuclei of EETC at doses of 100 and 200 mg/200 body weight (g) lower than negative control.Conclusion: The present study shows that the 70% EETC at dose of 100 and 200 mg/200 body weight (g) has hepatoprotective effect against alloxan induced liver damage.Â
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