EFFECT OF ETHANOLIC EXTRACT OF TINOSPORA CRISPA L FROM INDONESIA IN ALLOXAN INDUCED LIVER DAMAGE


Emsutrisna ., Fahrizal Aria Sahadewa, Ikbar Ardiansyah

Abstract


Objective: The objective of this study was to evaluate the hepatoprotective effect of Tinospora crispa L. (Bratawali).

Methods: Twenty four male rats wistar strain were divided into four groups. The serum alanine amino transferase (ALT) and aspartate aminotransferase (AST) of rats were measured (day 0). Rats then were injected by alloxan monohydrate at doses of 120 mg/200 bw (g) intraperitoneally. Four days later, the serum ALT and AST of rats were measured (second measurement/day 4) and then were treated by extract appropriate their groups. Group 1 was treated by 2 ml of distilled water orally; group 2,3 and 4 were treated by 70% ethanolic extract of T. crispa L. (EETC) at dose of 100; 200 and 400 mg/200 bw (g)/day respectively orally. After 10 d treatment, serum ALT and AST were measured (third measurement/day 14). At the end of this treatment, all rats were killed for histopathologic examination of their liver. The histopathologic examination was performed to assess the number of pyknotic nuclei, karyorrhexis nuclei and karyolysis nuclei.

Results: The result of this study showed that the ethanolic extract of T. crispa L at dose of 100 and 200 mg/200gbw can reduce blood ALT and AST significantly (P<0.05). From the histopathological examination, it was found that the number of pyknotic nuclei, karyorrhexis nuclei and karyolysis nuclei of EETC at doses of 100 and 200 mg/200 body weight (g) lower than negative control.

Conclusion: The present study shows that the 70% EETC at dose of 100 and 200 mg/200 body weight (g) has hepatoprotective effect against alloxan induced liver damage. 

 


Keywords


Tinospora crispa L, ALT, AST, Hepatoprotective

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References


Dweck AC, Cavin JP. A review of Andawali (Tinosporacrispa). Personal Care Magazine 2006;7:1–3.

Lawrence GHM. Taxonomy of vascular plants. The MacMillan Company, New York; 1951.

WHO, Cancer country profiles; 2014. Available from: http://www.who.int/cancer/country-profiles/en/ [Last accessed on 05 Aug 2016]

Harwoko, Choironi NA. Quality standardization of Brotowali (Tinospora crispa) stem extract. Trad Med J 2016;21: 6-11.

Dastur JF. Medicinal plants of India and Pakistan. DB.' ilala Sons and Co. Private LTD., Bombay; 1970.

Prastiwi R. Effect of hepatoprotector Brotowali (Tinospora cordifolia Miers) against hepatitis B virus. J Biomed 2011;4:5-10.

Harwoko, Choironi NA. Quality standardization of Brotowali (Tinospora crispa) stem extract. Trad Med J 2016;21: 6-11.

Ibahim MJ, Wan-Nor I’zzah WMZ, Narimah AHH, NurulAsyikin Z, Siti-NurShafinas SAR, Froemming GA. Anti-proliperative and antioxidant effects of Tinospora crispa (Bratawali). Biomed Res 2010;22:57-62.

Farokhi F, Farkha NK, Togmechi A, Band KS. Preventive effects of Prangos ferulacea (L.) Lindle on liver damage of diabetic rats induced by alloxan. Am J Physiol 2011;2:63-71.

Lucchesi AN, Cassettari LL, Spadella CT. Alloxan-induced diabetes causes morphological and ultrastructural changes in rat liver that resemble the natural history of chronic fatty liver disease in humans. J Diabetes Res 2015:1-11. http://dx.doi.org/10.1155/2015/494578

Sujith KC, Darwin R, Roosewelt C. Antidiabetic activity of methanolic extract of Butea frondosa leaves with its possible mechanism of action. Asian J Pharm Clin Res 2011;4:93-8

Rajaram K. Antioxidant and antidiabetic activity of Tectona grandis Linn. in alloxan induced albino rats. Asian J Pharm Clin Res 2013;6:174-7.

Sutrisna EM, Sujono TA, Dewi LI. Antidiabetic activity of ethanolic extract of Eugenia polyantha Wight leaf from indonesia in diabetic rat wistar strain induced by alloxan. Asian J Pharm Clin Res 2016;9:374-6.

Komalavalli T, Packia Lincy M, Muthukumarasamy S, Mohan VR. Antidiabetic and antihyperlipidaemic activity of Sonerila tinnevelliensis fischer whole plant in alloxan induced diabetic rats. Int J Chem Pharm Res 2015;7:53-7.

Rosen HR, Keefe EB. Evaluation of abnormal liver enzymes, use of liver tests and the serology of viral hepatitis: liver disease, diagnosis and management. 1st ed. New York; Churchill livingstone publishers; 2000. p. 24-35.

Friedman SF, Martin P, Munoz JS. Laboratory evaluation of the patient with liver disease. Hepatology, a textbook of liver disease. Philedelphia; Saunders publication; 2003;1:661-709.

Sherlock S. Assessment of liver function disease of liver and biliary system: Sheila Sherlock. 10th edn. London; Blackwell science ltd; 1997. p. 17-32.

Daniel SP, Marshall MK. Evaluation of the liver: laboratory tests. Schiff’s diseases of the liver. 8th edn. USA; JB Lippincott publications; 1999. p. 205-39.

Asayama K, Nyfeler F, English D, Pilkis SJ, Burr IM. Alloxan-induced free radical production in isolated cells selective effect on islet cells. Diabetes 1984;33:1006-12.

Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol 2001;54:176–86.

Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 2010;4:118–26.

Mohamad HNZ, Abidin NZ. Antioxidant activity of methanol extract of Tinosporacrispaand Tabernaemontana corymbosa. Sains Malaysiana 2013;42:697–706.

Zulkhairi A, Abdah MA, M Kamal NH, Nursakinah I, Moklas MAM, Hasnah B, et al. Biological properties of Tinosporacrispa (Akar Patawali) and Its antiproliferative activities on selected human cancer cell lines. Mal J Nutr 2008;14:173–87.

Fan W, Liu A, Wang W, Zheng G, Teng A. Hepatoprotective activity of CrPic against alloxan-induced hepatotoxicity in mice. Biol Trace Elem Res 2012;149:227–33.

Halliwell B. How to characterize an antioxidant-an update. Biochem Soc Symp 1995;61:73–101.




About this article

Title

EFFECT OF ETHANOLIC EXTRACT OF TINOSPORA CRISPA L FROM INDONESIA IN ALLOXAN INDUCED LIVER DAMAGE

Keywords

Tinospora crispa L, ALT, AST, Hepatoprotective

DOI

10.22159/ijpps.2017v9i8.17592

Date

01-08-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 8, 2017 Page: 65-68

Online ISSN

0975-1491

Statistics

36 Views | 47 Downloads

Authors & Affiliations

Emsutrisna .
Department of Pharmacology Faculty of Medicine of UniversitasMuhammadiyah Surakarta, Indonesia
Indonesia

Fahrizal Aria Sahadewa
Department of Pharmacology Faculty of Medicine of UniversitasMuhammadiyah Surakarta, Indonesia
Indonesia

Ikbar Ardiansyah
Department of Pharmacology Faculty of Medicine of UniversitasMuhammadiyah Surakarta, Indonesia
Indonesia


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