ISOLATION AND IDENTIFICATION OF A NEW PHYTOSTEROL FROM HOLOPTELEA INTEGRIFOLIA (ROXB) PLANCH LEAVES


Ravindra C. Sutar, Sanjay B. Kasture, V. K. Kalaichelvan

Abstract


Objective: The Plant Holoptelea Intergrifolia (Roxb.) Planch is being used for the treatment of various disorders since time immemorial in the indigenous system of medicine in India. The main objective of the work was to isolate a new phytosterol from petroleum ether extract of Holoptelea  Integrifolia leaves using Preparative Thin Layer Chromatography (TLC).

Methods: As per ICH guidelines we have Prepared Thin Layer Chromatographic plates for separation of a new phytosterol from Petroleum  ether extract of leaves of Holoptelea  Intergrifolia (Roxb.) Planch. The mobile phase used for separation of phytosterol consisted ofChloroform: Ethyl acetate, in the volume ratio of 4:6 (v/v), UV, LC/MS, IR and NMR spectral analytical techniques were used for identification and confirmation of structure of a new Phytosterol by Preparative TLC.

Results: Preliminary phytochemical analysis of petroleum ether extract of Holoptelea integrifolia leaves showed the presence of steroids, terpenoids, alkaloids, glycosides, flavonoids, proteins, tannins and carbohydrates. The isolated phytosterol designated as17-(6-(diethylamino) decan-3-yl)-10,13-dimethyl-12,13-dihydro-10H-cyclopenta[a] phenanthren-3-ol. It responded positively to Liebermann Burchard test indicating steroidal nature of the molecule.

Conclusion: On the basis of spectral data analysis and chemical reactions, the structure of a new phytosterol isolated by preparative TLC from petroleum ether extract of leaves of Holoptelea Integrifolia (Roxb.) Planch has been formulated by UV, LC/MS, IR and NMR spectral analysis as 17-(6-(diethylamino) decan-3-yl)-10,13-dimethyl-12,13-dihydro-10H-cyclopenta[a] phenanthren-3-ol

This is a new phytosterol isolated from plant source and being reported for the first time.

Keywords: Holoptelea Integrifolia leaves, UV, LC/MS, IR, NMR, Phytosterol.

 


Keywords


Holoptelea Integrifolia leaves, UV, LC/MS, IR, NMR, Phytosterol.

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About this article

Title

ISOLATION AND IDENTIFICATION OF A NEW PHYTOSTEROL FROM HOLOPTELEA INTEGRIFOLIA (ROXB) PLANCH LEAVES

Keywords

Holoptelea Integrifolia leaves, UV, LC/MS, IR, NMR, Phytosterol.

Date

29-07-2014

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 6, Issue 7, 2014 Page: 354-357

Online ISSN

0975-1491

Authors & Affiliations

Ravindra C. Sutar
Department of Pharmacology, Sanjivani College of Pharmaceutical Education and Research, Kopargaon. At- Sahajanandnagar, PostShinganapur 423603,Tal- Kopargaon, Dist-Ahmednagar, Maharashtra, India
India

Sanjay B. Kasture
Department of Pharmacology, Sanjivani College of Pharmaceutical Education and Research, Kopargaon. At- Sahajanandnagar, PostShinganapur 423603,Tal- Kopargaon, Dist-Ahmednagar, Maharashtra, India
India

V. K. Kalaichelvan
2Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram 608002, Tamil Nadu, India
India


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