• Nohair H. Sherif Drug Radiation Research Dept., National Centre for Radiation Research and Technology (NCRRT), Atomic Energy Authority, 3 Ahmed El Zomor st., El-Zohour Region, Nasr City, Cairo, Egypt
  • Asrar M. Hawas Drug Radiation Research Dept., National Centre for Radiation Research and Technology (NCRRT), Atomic Energy Authority, 3 Ahmed El Zomor st., El-Zohour Region, Nasr City, Cairo, Egypt
  • Walid E. Abdallah Phytochemistry Dept., National Research Centre, 33 El Bohouth st. (former El Tahrir st.), Dokki, Giza, Egypt
  • Ibrahim A. Saleh Phytochemistry Dept., National Research Centre, 33 El Bohouth st. (former El Tahrir st.), Dokki, Giza, Egypt
  • Khaled A. Shams Phytochemistry Dept., National Research Centre, 33 El Bohouth st. (former El Tahrir st.), Dokki, Giza, Egypt
  • Faiza M. Hammouda Phytochemistry Dept., National Research Centre, 33 El Bohouth st. (former El Tahrir st.), Dokki, Giza, Egypt


Objective: This study aimed to investigate the protective effect of Silybum marianum (S. marianum) seeds extract its oil fraction against damage effect of γ-radiation in female albino rats.

Methods: Ultrasonic-assisted extraction was used for the extraction of S. marianum seeds. Lipid patterns of S. marianum seeds oil were elucidated using gas chromatography-mass spectrometry (GC-MS). S. marianum seeds extract was analyzed using high-performance liquid chromatography (HPLC). Malondialdehyde (MDA), reduced glutathione (GSH) and metallothionein (MT) were estimated in heart and brain tissues of the examined rats. Lactate dehydrogenase (LDH) and creatine kinase-MB (CKMB) were measured in the serum of the examined rats, and the brain biomarkers; dopamine and serotonin were also measured.

Results: The oil was found to be rich in linoleic acid (58.20%) and arachidic acid (23.38%). S. marianum seeds extract revealed the presence of taxifolin and six main active constituents of silymarin, including silydianin, silychristin, silybin A, silybin B, isosilybin A and isosilybin B. Treatment of γ-radiation damage effect using S. marianum seeds extract and its oil fraction led to a significant reduction of MDA levels in heart (139.6 and 165.5 nmol/g, respectively) and brain (158.5 and 135.2 nmol/g, respectively) tissues, however, significant increase of GSH levels in heart (316.4 and 293 mg/g, respectively) and brain (210.4 and 227 mg/g, respectively) tissues was observed, also a significant increase of dopamine levels (85.27 and 65.74 ng/g, respectively) and MT levels of heart tissues (108.5 and 70.52 mg/g, respectively) was observed.

Conclusion: S. marianum seeds extract and its oil fraction showed a protective effect against γ-radiation-induced damage in heart and brain.


Keywords: Silybum marianum, γ-radiation, Heart, Brain, Female rats


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1. Murdoch CE, Zhang M, Cave AC, Shah AM. NADPH oxidase-dependent redox signalling in cardiac hypertrophy, remodelling and failure. Cardiovasc Res 2006;71:208-15.
2. Sawyer DB, Siwik DA, Xiao L, Pimentel DR, Singh K, Colucci WS. Role of oxidative stress in myocardial hypertrophy and failure. J Mol Cell Cardiol 2002;34:379-88.
3. Giordano FJ. Oxygen, oxidative stress, hypoxia, and heart failure. J Clin Invest 2005;115:500-8.
4. Huang KX, Gong JX, Xiong W, Yang LX, Wang F, Tao QF, et al. Preparation of silybin 23-esters and evaluation of their inhibitory ability against LPO and DNA protective properties. Chin Chem Lett 2009;20:1030-3.
5. Anderson FR, Fisher JL, Hara Y, Harris T, Mak BW, Melton DL, et al. Green tea catechins partially protect DNA from OH radical-induced strand breaks and base damage through fast chemical repair of DNA radicals. Carcinogenesis 2001;22:1189-93.
6. Maisin JR. Bacq and Alexander award lecture chemical radioprotection: past, present and future prospects. Int J Radiat Biol 1998;73:443–50.
7. Nair CKK, Parida DK, Nomura T. Radioprotectors in radiotherapy. J Radiat Res 2001;42:21-37.
8. Abbasi BH, Khan MA, Mahmood T, Ahmad M, Chaudhary MF. Shoot regeneration and free-radical scavenging activity in Silybum marianum L. PCTOC 2010;101:371-6.
9. Wellington K, Jarvis B. Silymarin: a review of its clinical properties in the management of hepatic disorders. BioDrugs 2001;15:465-89.
10. Flora K, Hahn M, Rosen H, Benner K. Milk thistle (Silybum marianum) for the therapy of liver disease. Am J Gastroenterol 1998;93:139-43.
11. Gazák R, Walterová D, Kren V. Silybin and silymarin-new and emerging applications in medicine. Curr Med Chem 2007;14:315-38.
12. Pietrangelo A, Montosi G, Garuti C, Contri M, Giovannini F, Ceccarelli D, et al. Iron-induced oxidant stress in nonparenchymal liver cells: mitochondrial derangement and fibrosis in acutely iron-dosed gerbils and its prevention by silybin. J Bioenerg Biomembr 2002;34:67-79.
13. Paulova J, Dvorak M, Kolouch F, Vanova L, Janeckova L. Verification of the hepatoprotective and therapeutic effect of silymarin in experimental liver injury with tetrachloromethane in dogs. Vet Med (Praha) 1990;35:629-35.
14. Sonnenbichler J, Goldberg M, Hane L, Madubunyi I, Vogl S, Zetl I. Stimulatory effect of Silibinin on the DNA synthesis in partially hepatectomized rat livers: non-response in hepatoma and other malign cell lines. Biochem Pharmacol 1986;35:538-41.
15. Sonnenbichler J, Zetl I. Biochemical effects of the flavonolignane silibinin on RNA, protein and DNA synthesis in rat livers. Prog Clin Biol Res 1986;213:319-31.
16. Crocenzi FA, Pellegrino JM, Sanchez EJ, Mottino AD, Garay EA, et al. Effect of silymarin on biliary bile salt secretion in the rat. Biochem Pharmacol 2000;59:1015-22.
17. Prabha T, Amit K, Manjoor A, Mishara KP. Radioprotection of plasmid and cellular DNA and swiss mice by silibinin. Mutat Res 2010;695:55-60.
18. Fu H, Katsumura Y, Lin M, Hata K, Muroya Y, Hatano Y. Fast repair activities towards dGMP hydroxyl radical adducts by silybin and its analogues. J Radiat Res 2008;49:609-14.
19. Galhardi F, Mesquita K, Monserrat JM, Barros DM. Effect of silymarin on biochemical parameters of oxidative stress in aged and young rat brain. Food Chem Toxicol 2009;47:2655-60.
20. Nazif NM. Development of production and application of pigments of certain local plants for food and pharmaceutical industries. Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Egypt; 1994.
21. Saleh IA, Vinatoru M, Mason TJ, Abdel-Azim NS, Aboutabl EA, Hammouda FM. Ultrasonic-assisted extraction and conventional extraction of silymarin from Silybum marianum seeds; a comparison. Res J Pharm Biol Chem Sci 2015;6:709-17.
22. El-Gabry MS, Abou-Safi HM, El-Yamany NA, Abdel-Hamid GR. Physiological studies on the efficacy of silymarin as antioxidant against the disorders in some blood constituents induced by irradiation in female rats. EJHM 2003;11:1-14.
23. Pesce A. Lactate dehydrogenase. In: Kaplan A. editor. Clinical Chemistry. Vol 438, St Louis. Toronto: The C. V. Mosby Co. Princeton; 1984. p. 1124–27.
24. Dawson DM, Eppenberger HM, Kaplan NO. Creatine kinase: evidence for a dimeric structure. Biochem Biophys Res Commun 1965;21:346-53.
25. Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;61:882-8.
26. Yoshioka T, Kawada K, Shimada T, Mori M. Lipid peroxidation in maternal and cord blood and protective mechanism against activated oxygen toxicity in the blood. Am J Obstet Gynecol 1979;135:372-6.
27. Scheuhammer AM, Cherian MG. Quantification of metallothioneins by a silver-saturation method. Toxicol Appl Pharmacol 1986;82:417-25.
28. Bienengräber M, Forderkunz S, Klein D, Summer KH. Determination of Cu-containing metallothionein: comparison by Ag saturation assay, thiomolybdate assay and enzyme-linked immunosorbent assay. Anal Biochem 1995;228:69-73.
29. Ciarolone AE. Further modification of a fluorometric method for analyzing brain amines. Microchem J 1978;23:9-12.
30. Kirkwood BR. Comparison of two means. In: Kirkwood BR. editor. Essentials of medical statistics. Oxford, England: Blackwell Scientific Publications; 1988. p. 41-45.
31. Takano H, Zou Y, Hasegawa H, Akazawa H, Nagai T, Komuro I. Oxidative stress-induced signal transduction pathways in cardiac myocytes: involvement of ROS in heart diseases. Antioxid Redox Signal 2003;5:789-94.
32. Demirci S, Nam J, Hubbs JL, Nguyen T, Marks LB. Radiation-induced cardiac toxicity after therapy for breast cancer: interaction between treatment era and follow-up duration. Int J Radiat Oncol Biol Phys 2009;73:980-7.
33. Darby S, Hill D, Auvinen A, Barros-Dios JM, Baysson H, Bochicchio F, Deo H, et al. Radon in homes and risk of lung cancer: collaborative analysis of individual data from 13 European case-control studies. Br Med J 2005;330:223-8.
34. Swerdlow AJ, Higgins CD, Smith P, Cunningham D, Hancock BW, Horwich A, et al. Myocardial infarction mortality risk after treatment for Hodgkin disease: a collaborative British cohort study. J Natl Cancer Inst 2007;99:206-14.
35. Tukenova M, Guibout C, Oberlin O, Doyon F, Mousannif A, Haddy N, et al. Role of cancer treatment in long-term overall and cardiovascular mortality after childhood cancer. J Clin Oncol 2010;28:1308-15.
36. Spitz DR, Azzam EI, Li JJ, Gius D. Metabolic oxidation/reduction reactions and cellular responses to ionizing radiation: a unifying concept in stress response biology. Cancer Metastasis Rev 2004;23:311-22.
37. Hauptmann M, Mohan AK, Doody MM, Linet MS, Mabuchi K. Mortality from diseases of the circulatory system in radiologic technologists in the United States. Am J Epidemiol 2003;157:239-48.
38. Howe GR, Zablotska LB, Fix JJ, Egel J, Buchanan J. Analysis of the mortality experience amongst U. S. nuclear power industry workers after chronic low-dose exposure to ionizing radiation. Radiat Res 2004;162:517-26.
39. Ivanov VK. Late cancer and noncancer risks among Chernobyl emergency workers of Russia. Health Phys 2007;93:470-9.
40. McGeoghegan D, Binks K, Gillies M, Jones S, Whaley S. The non-cancer mortality experience of male workers at British Nuclear Fuels plc, 1946-2005. Int J Epidemiol 2008;37:506-18.
41. Muirhead CR, O’Hagan JA, Haylock RG, Phillipson MA, Willcock T, Berridge GLC, et al. Mortality and cancer incidence following occupational radiation exposure: third analysis of the National Registry for Radiation Workers. Br J Cancer 2009;100:206-12.
42. Cai L, Satoh M, Tohyama C, Cherian MG. Metallothionein in radiation exposure: its induction and protective role. Toxicology 1999;132:85-98.
43. Lu P, Mamiya T, Lu LL, Mouri A, Zou L, Nagai T, et al. Silibinin prevents amyloid beta peptide-induced memory impairment and oxidative stress in mice. Br J Pharmacol 2009;157:1270-7.
44. Valenzuela A, Guerra R. Protective effect of the flavonoid silybin dihemisuccinate on the toxicity of phenylhydrazine on rat liver. FEBS Lett 1985;181:291-4.
45. Fu H, Lin M, Katsumura Y, Yokoya A, Hata K, Muroya Y, et al. Protective effects of silybin and analogues against X-ray radiation–induced damage. Acta Biochim Sin 2010;42:489-95.
46. Lu P, Mamiya T, Lu L, Mouri A, Niwa M, Kim HC, et al. Silibinin attenuates cognitive deficits and decreases of dopamine and serotonin induced by repeated methamphetamine treatment. Behav Brain Res 2010;207:387-93.
47. Nencini C, Giorgi G, Micheli L. Protective effect of silymarin on oxidative stress in rat brain. Phytomedicine 2007;14:129-35.
48. Jones DP. Redefining oxidative stress. Antioxid Redox Signal 2006;8:1865-79.
49. Pérez HJ, Carrillo SC, García E, Ruiz-Mar G, Pérez-Tamayo R, Chavarría A. Neuroprotective effect of silymarin in a MPTP mouse model of Parkinson's disease. Toxicology 2014;319:38-43.
50. Thakare VN, Dhakane VD, Patel BM. Potential antidepressant-like activity of silymarin in the acute restraint stress in mice: modulation of corticosterone and oxidative stress response in cerebral cortex and hippocampus. Pharmacol Rep 2016;68:1020-7.
51. Raza SS, Khan MM, Ashafaq M, Ahmad A, Khuwaja G, Khan A, et al. Silymarin protects neurons from oxidative stress associated damages in focal cerebral ischemia: a behavioral, biochemical and immunohistological study in Wistar rats. J Neurol Sci 2011;309:45-54.
52. Ligeret H, Brault A, Vallerand D, Haddada Y, Haddad PS. Antioxidant and mitochondrial protective effects of silibinin in cold preservation–warm reperfusion liver injury. J Ethnopharmacol 2008;115:507-14.
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How to Cite
Sherif, N. H., A. M. Hawas, W. E. Abdallah, I. A. Saleh, K. A. Shams, and F. M. Hammouda. “POTENTIAL ROLE OF MILK THISTLE SEED AND ITS OIL EXTRACTS AGAINST HEART AND BRAIN INJURIES INDUCED BY γ-RADIATION EXPOSURE”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 9, no. 7, July 2017, pp. 52-58, doi:10.22159/ijpps.2017v9i7.18046.
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