ANTIDYSLIPIDEMIA AND ANTIOXIDANT ACTIVITY OF ANDROGRAPHOLIDE COMPOUND FROM SAMBILOTO (ANDROGRAPHIS PANICULATA) HERB


Ni Kadek Warditiani, Ni Made Pitri Susanti, Cokorda Istri Sri Arisanti, Ni Putu Rika Dharma Putri, I Made Agus Gelgel Wirasuta

Abstract


Objective: The aim of this study was to observe the antidyslipidemic and antioxidant activity of andrographolide (AND) compound from Sambiloto (Andrographis paniculata) herb. The antidyslipidemic activity could be described by lowering TC (total cholesterol), TG (triglyceride), and LDL (low-density lipoprotein) level in the blood and by increasing HDL (high-density lipoprotein) level in the blood. The antioxidant activity could be described by inhibiting the ox-LDL (oxidized low-density lipoprotein) formation.

Methods: AND was separated from the sambiloto ethanolic extract. Atherosclerosis in rats was induced by rich fat food (5% yolk, 10% lard), 1% calcium and 20.000 IU vitamin D3 for 90 d. The rats were treated with AND compound on the 61st day for 30 d. On the 90th day, the blood of rats had been collected, and its blood vessel was also extracted. Antidyslipidemic activity was measured by estimating the level of TC, TG, LDL and HDL in the blood. Antioxidant activity was measured by calculating the ox-LDL concentration in blood and histopathology parameter.

Results: 18 mg/kg BW AND could reduce the level TC, TG, and LDL in blood, and could increase HDL blood level significantly (p<0.05). This compound can also significantly inhibit LDL oxidation (p<0.05), and the histopathology result showed that this compound could protect the vessel from adhesive lipid plaques.

Conclusion: AND compound from sambiloto herb showed the antidyslipidemic and antioxidant effect in atherogenic rats.


Keywords


Andrographolide, Antidyslipidemic, Antioxidant, Atherosclerosis, Sambiloto Herb

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References


Akbar S. Andrographis paniculata: a review of pharmacological activities and clinical effects. Napa: LLC; 2011. p. 66-77.

Negi AS, Kumar JK, Luqman S, Sbanker K, Gupta MM, Kbanuja SPS. Recent advances in plant hepatoprotectives: a chemical and biological profile of some important leads. Med Res Rev 2008;28:821.

Roxas M, Jurenka J. Colds and influenza: a review of diagnosis and conventional, botanical and nutritional considerations. Altern Med Rev 2007;12:25-48.

Cheung HY, Cheung CS, Kong CK. Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography. J Chromatogr 2001;930:171-6.

Brown SM, Goldstein LJ. A receptor-mediated pathway for cholesterol homeostasis. Science 1986;232:34-7.

William I. The pathology of atherosclerosis: plaque development and plaque responses to medical treatment. Am J Med 2009;122:S3–14.

Parthasarathy S, Wieland E, Steinberg D. A role for endothelial cell lipoxygenase in the oxidative modification of low density lipoprotein. Proc Natl Acad Sci U S A 1989;86:1046-50.

Martha S, Akula A, Yellu NR. Anti-atherosclerotic effect of atorvastatin and clopidrogel alone and combination in rats. Int J Evol Biol 2008;46:698-703.

Takahashi K, Takeya M, Sakashita N. Multifunctional roles of macrophages in development and progression of atherosclerosis in human and experimental animals. Med Electron Microsc 2002;35:179-203.

Bennani-Kabchi NL, Kehel F, El B, Fdhil H, Amarti A, Saidi AG. New model of atherosclerosis in insulin resistant sand rats: hypercholesterolemia combined with D2 vitamin. Atherosclerosis 2000;150:55-61.

Wongkittipong R, Prat S, Gourdon C. Solid-liquid extraction of andrographolide from plants experimental study, kinetic reaction and model. Sep Purif Technol 2004;40:147-54.

Hermanto S, Muawanah A, Harahap R. Kadar dan karakteristik lemak hewani (Ayam, Sapi dan Babi) hasil analisa FTIR dan GCMS. J Valensi 2008;1:102-9.

Harini M, Astirin OP. Kadar kolesterol darah tikus putih (Rattus novergicus) hiperkolesterolemik setelah perlakuan VCO. Bioteknologi 2009;6:55-62.

Lakshmia V, Srivastav S, Khanna AK, Mahdi AA, Agarwal SK. Lipid lowering potential of Andrographis paniculata (Nees). J Phytopharmacol 2014;3:124-9.

Ravi K, Ramachandran B, Subramanian S. Effect of Eugeniajambolana seed kernel on antioxidant defensesystem in streptozotocin induced diabetes in rats. Life Sci 2004;75:2717-31.

Brown SL. Lowered serum cholesterol and low mood. Br Med J 1996;313:637-8.

Shahidi F. Natural Antioxidant, Chemistry, Health Effect and Application, AOCS. Press Champaign, Illinois; 1997. p. 1-23.

Huidrom S, Deka M. Determination of antioxidant property of Andrographis paniculata. Indian J Drugs Diseases 2012;1:12-7.

Maiolino G, Rossitto G, Caielli P, Bisogni V, Rossi GP, Calo LA. The role of oxidized low-density lipoproteins in atherosclerosis: the myths and the facts. Med Inflamm 2013. p. 1-13.

Steinberg D, Witztum JL. Lipoproteins and atherogenesis. Curr Concepts J Am Med Assoc 1990;264:3047–52.

Brand K, Page S, Rogler G, Bartsch A, Brandl R, Knuechel R, et al. Activated transcription factor nuclear factor-kappa B is present in the atherosclerotic lesion. J Clin Invest 1996;97:1715–22.

Patel D, Hirangi, Gaurang BS, Vandit T. Investigation of HMG Co A reductase inhibitory activity of antihyperlipidemic herbal drugs in vitro study. Asian J Exp Biol Sci 2011;2:63-8.

Kesh SB, Sarkar D, Manna K. High-fat diet-induced oxidative stress and its impact on metabolic syndrome: a review. Asian J Pharm Clin Res 2016;9:47-52.




About this article

Title

ANTIDYSLIPIDEMIA AND ANTIOXIDANT ACTIVITY OF ANDROGRAPHOLIDE COMPOUND FROM SAMBILOTO (ANDROGRAPHIS PANICULATA) HERB

Keywords

Andrographolide, Antidyslipidemic, Antioxidant, Atherosclerosis, Sambiloto Herb

DOI

10.22159/ijpps.2017v9i7.18109

Date

01-07-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 7, 2017 Page: 59-65

Online ISSN

0975-1491

Statistics

95 Views | 114 Downloads

Authors & Affiliations

Ni Kadek Warditiani
Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana University, Bali, Indonesia 80361
Indonesia

Ni Made Pitri Susanti
Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana University, Bali, Indonesia 80361
Indonesia

Cokorda Istri Sri Arisanti
Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana University, Bali, Indonesia 80361
Indonesia

Ni Putu Rika Dharma Putri
Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana University, Bali, Indonesia 80361
Indonesia

I Made Agus Gelgel Wirasuta
Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana University, Bali, Indonesia 80361
Indonesia


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