A SIMPLE (HPLC–UV) METHOD FOR THE QUANTIFICATION OF COLCHICINE IN BULK AND ETHOSOMAL GEL NANO-FORMULATION AND ITS VALIDATION


Ibrahim M. Abdulbaqi, Yusrida Darwis, Nurzalina Abdul Karim Khan, Reem Abou Assi, Gabriel Onn Kit Loh

Abstract


Objective: To develop and validate a stability-indicating reversed phase high-performance liquid chromatography (RP-HPLC) method for the determination of colchicine in bulk and ethosomal gel nano-formulation.

Methods: The chromatographic conditions were optimized using stainless steel Hypersil Gold C-18 analytical column with the dimensions of 250 mm x 4.6 mm ID x 5 µm. The mobile phase consisted of acetonitrile and ammonium acetate buffer (20 mmol/l, pH=4.85) in the ratio of 32:68 v/v. The flow rate was set at 1 ml/min and the detection wavelength was 353 nm. The column was maintained at 30 °C and the injection volume was 10 µl. The stability of colchicine in different conditions was investigated by exposing the drug to stress degradation using acid, base, oxidation, heat and light.

Results: There was no interference from excipients, impurities, dissolution media or degradation products at the retention time of colchicine 5.9 min indicating the specificity of the method. The limit of detection (LOD) and the limit of quantification (LOQ) were 8.64 ng/ml and 26.17 ng/ml respectively. The drug showed good stability under heat, acid, oxidation and light, but substantial degradation was observed under alkali condition. The procedure was validated for specificity, linearity, accuracy and precision.

Conclusion: A simple, rapid, specific and stability-indicating HPLC–UV method for the determination of colchicine in the pure and ethosomal gel was successfully developed. The developed method was statistically confirmed to be accurate, precise, and reproducible.


Keywords


Colchicine, Ethosomal gel, HPLC-UV, Specificity

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About this article

Title

A SIMPLE (HPLC–UV) METHOD FOR THE QUANTIFICATION OF COLCHICINE IN BULK AND ETHOSOMAL GEL NANO-FORMULATION AND ITS VALIDATION

Keywords

Colchicine, Ethosomal gel, HPLC-UV, Specificity

DOI

10.22159/ijpps.2017v9i7.18336

Date

01-07-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 7, 2017 Page: 72-78

Online ISSN

0975-1491

Statistics

210 Views | 151 Downloads

Authors & Affiliations

Ibrahim M. Abdulbaqi
School of pharmaceutical sciences, Universiti Sains Malaysia, 11800 Minden, Pulau Penang, Malaysia
Malaysia

Yusrida Darwis
School of pharmaceutical sciences, Universiti Sains Malaysia, 11800 Minden, Pulau Penang, Malaysia
Malaysia

Nurzalina Abdul Karim Khan
School of pharmaceutical sciences, Universiti Sains Malaysia, 11800 Minden, Pulau Penang, Malaysia

Reem Abou Assi
School of pharmaceutical sciences, Universiti Sains Malaysia, 11800 Minden, Pulau Penang, Malaysia

Gabriel Onn Kit Loh
School of pharmaceutical sciences, Universiti Sains Malaysia, 11800 Minden, Pulau Penang, Malaysia


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