STUDY ON IN VITRO CYTOTOXICITY OF PAPAIN AGAINST LIVER CANCER CELL LINE HEP G2

  • M Akila Department of Biotechnology, Periyar Maniammai University, Vallam, Thanjavur 613403, India.
  • A Sushama Department of Biotechnology, Periyar Maniammai University, Vallam, Thanjavur 613403, India.
  • Kumaresan Ramanathan Department of Biotechnology, Periyar Maniammai University, Vallam, Thanjavur 613403, India.

Abstract

Objective: Cancer is a devastating public health problem all over the world. Herbal medicines have a vital role in the prevention and treatment of cancer. Papain is a proteolytic enzyme and a phyto therapeutic agent which highly appreciated in the medical field that prevents several chronic diseases such as cardiovascular disease, cancer and diabetes. The aim of the study describes about the cytotoxicity of papain against liver cancer cell line HepG2.

Methods: In different concentrations of samples have been taken for cytotoxicity analysis using the MTT assay and calculate the percentage of cell viability.

Results: The 50% inhibition concentration (IC50) of a sample was 125µg/ml and their cell viability percentage was 49.20. The 85% of cytotoxicity has been observed in 1000 µg/ml of papain against HepG2 cell line than other concentrations.

Conclusion: 1000 µg/ml of papain has maximum (85%) cytotoxicity effect against liver cancer cell line hepG2.

 

Keywords: Papain, HepG2, Cell viability, Cytotoxicity.

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References

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How to Cite
Akila, M., A. Sushama, and K. Ramanathan. “STUDY ON IN VITRO CYTOTOXICITY OF PAPAIN AGAINST LIVER CANCER CELL LINE HEP G2”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 9, 1, pp. 160-1, https://innovareacademics.in/journals/index.php/ijpps/article/view/1868.
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