STUDY ON IN VITRO CYTOTOXICITY OF PAPAIN AGAINST LIVER CANCER CELL LINE HEP G2
Keywords:Papain, HepG2, Cell viability, Cytotoxicity
Objective: Cancer is a devastating public health problem all over the world. Herbal medicines have a vital role in the prevention and treatment of cancer. Papain is a proteolytic enzyme and a phyto therapeutic agent which highly appreciated in the medical field that prevents several chronic diseases such as cardiovascular disease, cancer and diabetes. The aim of the study describes about the cytotoxicity of papain against liver cancer cell line HepG2.
Methods: In different concentrations of samples have been taken for cytotoxicity analysis using the MTT assay and calculate the percentage of cell viability.
Results: The 50% inhibition concentration (IC50) of a sample was 125Âµg/ml and their cell viability percentage was 49.20. The 85% of cytotoxicity has been observed in 1000 Âµg/ml of papain against HepG2 cell line than other concentrations.
Conclusion: 1000 Âµg/ml of papain has maximum (85%) cytotoxicity effect against liver cancer cell line hepG2.
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