GENETIC DIVERSITY IN ACCESSIONS OF INDIAN TURMERIC (CURCUMA LONGA L.) USING RAPD MARKERS


Kamran Ashraf, Altaf Ahmad, Syed Adnan Ali Shah, Mohd Mujeeb

Abstract


Objective: The present investigation was undertaken for identification and assessment of eight accessions of Curcuma longa collected from all ecological zones in India by random amplification of polymorphic DNA (RAPD) markers.

Methods: DNA was isolated using modified cetyl trimethyl ammonium bromide (CTAB) method. Polymerase chain reaction (PCR) was performed according to the method based on Williams et al. and data analysis was done using Alpha Imager EC software.

Results: Eleven out of twenty primers screened were informative and produced 150 amplification products among which 132 products (88%) were found to be polymorphic. The percentage polymorphism of all 08 accessions ranged from 44.44% to 100%. A total of 150 amplification products were scored with an average frequency of 13.63 bands per primer. Most of the RAPD markers studied showed a different level of genetic polymorphism. The data of 150 RAPD bands were used to generate Jaccard’s similarity coefficients and to construct a dendrogram by means of UPGMA.

Conclusion: Results shows that C. longa undergoes genetic variation due to a wide range of ecological conditions within distribution area of its population in India. This investigation as an understanding of the level and partitioning of genetic variation within the accessions and would provide an important input into determining efficient management strategies and will help to breeders for turmeric improvement program.


Keywords


Curcuma longa, RAPD, CTAB, UPGMA, India

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About this article

Title

GENETIC DIVERSITY IN ACCESSIONS OF INDIAN TURMERIC (CURCUMA LONGA L.) USING RAPD MARKERS

Keywords

Curcuma longa, RAPD, CTAB, UPGMA, India

DOI

10.22159/ijpps.2017v9i10.18715

Date

02-10-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol. 9, Issue 10, 2017 Page: 288-291

Online ISSN

0975-1491

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Authors & Affiliations

Kamran Ashraf
Faculty of Pharmacy, Universiti Teknologi MARA, Puncak Alam Campus, Bandar Puncak Alam, Selangor Darul Ehsan, Malaysia
Malaysia

Altaf Ahmad
Department of Botany, Aligarh Muslim University Aligarh, Aligarh, Uttar Pradesh, India

Syed Adnan Ali Shah
Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA, Puncak Alam Campus, Bandar Puncak Alam, Selangor Darul Ehsan, Malaysia

Mohd Mujeeb
Bioactive Natural Product Laboratory,Department of Pharmacognosy and Phytochemistry ,Faculty of Pharmacy, Jamia Hamdard Univ,New Delhi-62, India
India


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