BIOCHEMICAL AND HISTOPATHOLOGICAL CHANGES OF TRADITIONAL SIDDHA FORMULATION ON CCL4 INDUCED LIVER FIBROSIS IN RATS


Diwakar Manickam, Sri Kamatchi Priya Ramamoorthy, B. Santhosh Kumar, Samu Subramaniam, Shyama Subramaniam

Abstract


ABSTRACT

Objective:

The main objective of this study is to evaluate therapeutic activity of traditional siddha formulation on CCl4 induced liver fibrosis in rats.

Methods:

Plant materials collected, shade dried, mixed equal proportion. This mixture was used for extract or kasayam preparation. Twenty four male Wistar albino rats were randomly divided into four groups of six rats each. The normal control group was allowed free access to food and water. Drug control group was allowed to daily intragastric administration of traditional siddha formulation 400mg per kg b.wt per day. Liver damage was performed in the remaining two groups with an i.p.injection of a 1.0 mL/kg b.wt CCl4 and olive oil (2:3 v/v) mixture, twice weekly for 8 weeks. All rats, with the exception of the injury model group, were intragastrically administered traditional siddha formulation (TSF) dosage 400 mg/ kg b.wt /day from 5th week to end of experiment of CCl4 intoxicated rats. The serum levels of hepatic markers and hydroxyproline (Hyp) in the rat livers were measured. Histopathological changes in the liver were assessed for each group using H&E staining and Masson Trichrome examination.

Results:

Traditional siddha formulation significantly reverse the liver function in CCl4 intoxicated rats correlate with both biochemical and histopathological changes

Conclusion:

Traditional siddha formulation a promising drug for treating liver fibrosis.

Keywords: Traditional siddha formulation, Liver fibrosis, CCl4 and Vaithiyars


Keywords


Traditional siddha formulation, Liver fibrosis, CCl4 and Vaithiyars

| PDF | HTML |

References


Friedman SL. Hepatic stellate cells: protean, multifunctional, and enigmatic cells of the liver. Physiol Rev 2008;88:125-72.

Gines P, Cardenas A, Arroyo V, Rodes J. Management of cirrhosis and ascites. N Engl J Med 2004;350:1646–54.

Friedman SL. Liver fibrosis-from bench to bedside. J Hepatol 2003;38:S38–53.

UE Lee, SL Friedman. Mechanisms of hepatic fibrogenesis. Best Practice Res Clin Gastroenterol 2011;25:195–206.

Weiler-Normann C, Herkel J, Lohse AW. Mouse models of liver fibrosis. Z Gastroenterol 2007;45:43–50.

Hamdy N, El-Demerdash E. New therapeutic aspect for carvedilol: antifibrotic effects of carvedilol in chronic carbon tetrachloride-induced liver damage. Toxicol Appl Pharmacol 2012;261:292–9.

Shi J, Aisaki K, Ikawa Y, Wake K. Evidence of hepatocyte apoptosis in rat liver after the administration of carbon tetrachloride. Am J Pathol 1998;153:515–25.

Maling HM, Eichelbaum FM, Saul W, Sipes IG, Brown EA, Gillette JR. Nature of the protection against carbon tetrachloride-induced hepatotoxicity produced by pretreatment with dibenamine [N-(2-chloroethyl) dibenzylamine]. Biochem Pharmacol 1974;23:1479–91.

Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Mini-review. Chem Biol Interact 2006;160:1-40.

Abdelaziz DHA, Ali SA. The protective effect of Phoenix dactylifera L. seeds against CCl4-induced hepatotoxicity in rats. J Ethnopharmacol 2014;155:736–43.

Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. 3rd edition. Oxford, UK: Clarendon Press; 1999.

Beckman KB, Ames BN. The free radical theory of aging matures. Physiol Rev 1998;78:547–81.

Mutheeswaran S, Pandikumar P, Chellappandian M, Ignacimuthu S, Duraipandiyan V, Logamanian M. Consensus analysis of sastric formulations used by non-institutionally trained siddha medical practitioners of Vir-udhunagar and Tirunelveli districts of Tamil Nadu, India. J Ethnopharmacol 2014;153:290–6.

Sri kamatchi priya Ramamoorthy, Diwakar Manickam, Samu subramaniam, Shyama Subramaniam. Standardisation and phytochemical screening of traditional formulation. Int J Curr Pharm Res 2017;9:70-4.

Vaidya Ratnam KS, Murugesa Mudaliar. Gunapadam. 1st edition. (Siddha Materia Medica); 1936. p. 229, 345, 459, 520, 720.

Guo-Yu Li, Hong-Ying Gao, Jian Huang, Jin Lu, Jing-Kai Gu, et al. Hepatoprotective effect of Cichorium intybus L,a traditional Uighur medicine, against carbon tetrachloride-induced hepatic fibrosis in rats. World J Gastroenterol 2014;20:4753-60.

Fiorucci S, Antonelli E, Distrutti E, Severino B, Fiorentina R, Baldoni M, et al. PAR1 antagonism protects against experimental liver fibrosis. Role of proteinase receptors in stellate cell activation. Hepatology 2004;39:365–75.

Shahjahan M, Sabitha KE, Jainu M, Shyamala Devi CS. Effect of Solanum trilobatum against carbon tetrachloride induced hepatic damage in albino rats. Indian J Med Res 2004;120:194–8.

Zimmerman HJ, Kodera Y, West M. Effects of carbon tetrachloride. Poisoning on the plasma levels of cytoplasmic and mitochondrial enzymes in animals with nutritional fatty metamorphosis. J Lab Clin Med 1965;66:324–33.

Murray RK. Porphyrins and bile pigments. In: Murry RK, Granner DK, Mayes PA, Rodwell VW. editors. Harper’s illustrated biochemistry. 26th Ed. New York: McGraw-Hill Inc; 2003. p. 270–85.

Khan AA, Alzohairy M. Hepatoprotective effects of camel milk agains CCL4-induced hepatotoxicity in Rats. Asian J Biochem 2011;6:171–80.

Smyth R, Munday MR, York MJ, Clarke CJ, Dare T, Turton JA. Comprehensive Characterization of serum clinical chemistry parameters and the identification of urinary superoxide dismutase in a carbon tetrachloride-induced model of hepatic fibrosis in the female Hanover Wistar rat. Int J Exp Pathol 2007;88:361–76.

¬Wang Y, Cheng M, Zhang B, Nie F, Jiang H. Dietary supplementation of blueberry juice enhances hepatic expression of metallothionein and attenuates liver fibrosis in rats. PLoS ¬¬¬¬One 2013;8:e58659.




About this article

Title

BIOCHEMICAL AND HISTOPATHOLOGICAL CHANGES OF TRADITIONAL SIDDHA FORMULATION ON CCL4 INDUCED LIVER FIBROSIS IN RATS

Keywords

Traditional siddha formulation, Liver fibrosis, CCl4 and Vaithiyars

Date

01-07-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 7, 2017 Page: 120-123

Online ISSN

0975-1491

Statistics

29 Views | 15 Downloads

Authors & Affiliations

Diwakar Manickam
Research Scholar, Department of Biochemistry, Regenix Super Speciality Laboratories, affiliated to University of Madras, Chennai, Tamilnadu, India
India

Sri Kamatchi Priya Ramamoorthy
Research Scholar, Department of Biochemistry, Regenix Super Speciality Laboratories, affiliated to University of Madras, Chennai, Tamilnadu, India
India

B. Santhosh Kumar
Associate Professor, Consultant, Lab Services, Apollo Hospital, Chennai, Tamilnadu, India
India

Samu Subramaniam
Lab Director, Head of the Department, Department of Biochemistry, Regenix Super Speciality Laboratories, Chennai, Tamilnadu, India
India

Shyama Subramaniam
Associate Professor, Consultant, Lab Services, Apollo Hospital, Chennai, Tamilnadu, India
India


Article Tools



Refbacks

  • There are currently no refbacks.