ANTIHEPATOTOXIC POTENTIALITY OF 2-3-6 TRIMETHYLOCT-6-ENAL AGAINST PARACETAMOL INDUCED LIVER DYSFUNCTION


Suparna Datta, Manabendra Dutta Choudhury

Abstract


Objective: We investigated the liver protective activity of 2-3-6 trimethyloct-6-enal from the methanol extract of Pajanelia longifolia (Willd.) K. Schuman. The liver protective activity of 2,3,6 trimethyloct-6-enal was evaluated against paracetamol (2 mg/kg body weight per orally) induced liver toxicity in swiss albino mice.

Methods: Considering the Spectral data (IR spectrum, 1HNMR spectrum and 13C NMR spectrum) the predictable structure of 2,3,6 trimethyloct-6-enal was elucidated. To study the liver protective activity of the compound, Swiss albino mice of either sex were divided into six groups and treated for 5 d. Group I and II served as normal and toxic control, Group III were treated with Silymarin as a standard drug (50 mg/kg), and Group IV to VI was treated with 2-3-6 trimethyloct-6-enal at the dose of 50 mg/kg, 150 mg/kg and 250 mg/kg b.w. p. o. respectively. The liver protective activity of the compound was measured on biochemical parameters such as aspertate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total bilirubin (TB), triglycerides (TGL), total cholesterol (TC) and protein. Further antioxidant activity of the compound was also measured on antioxidant enzymatic and non-enzymatic levels such as reduced glutathione (GSH), lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)).

Results: The study revealed that the compound has protective activity at the dose of 50, 150 and 250 mg/kg b.w. p. o. against paracetamol induced toxicity. In some biochemical parameters such as aspartate amino transferase and bilirubin, the compound has showed better result at a dose of 150 mg/kg compared to standard drug silymarin (value of aspartate amino transferase (compound) =71.10±0.12, (toxic) = 173.43±1.21, (silymarin) =79.86±0.02and total bilirubin (compound) = 1.04±0.11), (toxic) = 2.69±0.02, (silymarin) ==1.11±0.01. The findings were also confirmed by histopathological observations.

Conclusion: 2,3,6 trimethyloct-6-enal from Pajanelia longifolia may be considered as a potent liver protective agent.


Keywords


2,3,6 trimethyloct-6-enal, Liver protective activity, Paracetamol, Histopathological observations, Pajanelia longifolia (Willd.) K. Schuman

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About this article

Title

ANTIHEPATOTOXIC POTENTIALITY OF 2-3-6 TRIMETHYLOCT-6-ENAL AGAINST PARACETAMOL INDUCED LIVER DYSFUNCTION

Keywords

2,3,6 trimethyloct-6-enal, Liver protective activity, Paracetamol, Histopathological observations, Pajanelia longifolia (Willd.) K. Schuman

DOI

10.22159/ijpps.2017v9i9.19075

Date

13-07-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 9, 2017 Page: 59-63

Online ISSN

0975-1491

Statistics

63 Views | 23 Downloads

Authors & Affiliations

Suparna Datta
Ethnobotany and Medicinal Plant Research Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar, Assam, India
India

Manabendra Dutta Choudhury
Ethnobotany and Medicinal Plant Research Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar, Assam, India
India


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