DETERMINATION OF OCTANOL-WATER PARTITION COEFFICIENT OF NOVEL COUMARIN BASED ANTICANCER COMPOUNDS BY REVERSED-PHASE ULTRA-FAST LIQUID CHROMATOGRAPHY


Venkata Sairam K., Gurupadayya Bm, Vishwanathan B. Iyer, Chandan R. S.

Abstract


Objective: The present study aims at the development of a reversed phase ultra-fast liquid chromatography (RP-UFLC) method for measurement of the lipophilicity (log P) between n-octanol and water for the newly synthesized coumarin derivatives in our laboratory.

Methods: The synthesized compounds were dissolved in methanol and analyzed using XTerra RP18 column as the stationary phase and a mixture of methanol (0.25% v/v octanol) and buffer as the mobile phase with isocratic elution.

Results: In this study we concentrated on the relationship between a reversed-phase ultra-fast liquid chromatography (RP-UFLC) retention parameters and log P of our synthesized compounds. Furthermore, a good correlation and very close values were obtained between the experimentally determined log P values and values obtained from Chemdraw.

Conclusion: The developed method was found to be insensitive to any of the impurities present and moreover it requires very little sample for analysis.


Keywords


RP-UFLC, Synthetic, Coumarin, Log P, Octanol-Water

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About this article

Title

DETERMINATION OF OCTANOL-WATER PARTITION COEFFICIENT OF NOVEL COUMARIN BASED ANTICANCER COMPOUNDS BY REVERSED-PHASE ULTRA-FAST LIQUID CHROMATOGRAPHY

Keywords

RP-UFLC, Synthetic, Coumarin, Log P, Octanol-Water

DOI

10.22159/ijpps.2017v9i8.19128

Date

01-08-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 8, 2017 Page: 98-104

Online ISSN

0975-1491

Statistics

62 Views | 38 Downloads

Authors & Affiliations

Venkata Sairam K.
Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Jagadguru Sri Shivarathreeshwara University, Mysuru 570015, India
India

Gurupadayya Bm
Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Jagadguru Sri Shivarathreeshwara University, Mysuru 570 015, India
India

Vishwanathan B. Iyer
Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Jagadguru Sri Shivarathreeshwara University, Mysuru 570 015, India
India

Chandan R. S.
Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Jagadguru Sri Shivarathreeshwara University, Mysuru 570 015, India
India


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