• Prachi Patil School of Pharmacy, Devi Ahilya University, Indore, India
  • Rajesh Sharma School of Pharmacy, Devi Ahilya University, Indore, India
  • Tushar Banerjee School of Life Sciences, Devi Ahilya University, Indore-452001, India
  • Shridhar Patil School of Life Sciences, Devi Ahilya University, Indore 452001, India


Objective: The present study was aimed at developing a rapid, cost effective and accurate method for quantification of exemestane using thin layer chromatography (TLC) separation followed by image analysis and to test it for monitoring the accumulation of exemestane during microbial bioconversion.

Methods: After microbial bioconversion and TLC separation of products formed, exemestane was quantified using ImageQuant TL v2003 image analysis software and the results were compared with high performance liquid chromatography (HPLC) analysis.

Results: The percentage error between TLC and HPLC analyses was ranged from (-) 5.18 to (+) 5.51. Bacterial strains Arthrobacter simplex IAM 1660, Nocardia sp. MTCC 1534, Pseudomonas putida MTCC 1194 and Rhodococcus rhodochorus MTCC 291 respectively yielded 79.7 (72 h), 63.9 (72 h), 69.8 (96 h) and 83.2 (96 h) mole percent bioconversion of 6-methylene androstenedione to exemestane.

Conclusion: Rhodococcus rhodochorus MTCC 291 was found to be the most suitable organism for the bioconversion and may be used to develop an eco-friendly route to replace chemical synthesis that eliminates the use of toxic chemicals and side products.

Keywords: TLC image analysis, 6-Methylene androstenedione, C-1(2)-Dehydrogenation, Exemestane, Rhodococcus rhodochorus MTCC 291


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How to Cite
Patil, P., R. Sharma, T. Banerjee, and S. Patil. “QUANTIFICATION OF EXEMESTANE ACCUMULATION DURING MICROBIAL BIOCONVERSION BY TLC IMAGE ANALYSIS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 9, no. 8, Aug. 2017, pp. 105-9, doi:10.22159/ijpps.2017v9i8.19201.
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