QUANTIFICATION OF EXEMESTANE ACCUMULATION DURING MICROBIAL BIOCONVERSION BY TLC IMAGE ANALYSIS


Prachi Patil, Rajesh Sharma, Tushar Banerjee, Shridhar Patil

Abstract


Objective: The present study was aimed at developing a rapid, cost effective and accurate method for quantification of exemestane using thin layer chromatography (TLC) separation followed by image analysis and to test it for monitoring the accumulation of exemestane during microbial bioconversion.

Methods: After microbial bioconversion and TLC separation of products formed, exemestane was quantified using ImageQuant TL v2003 image analysis software and the results were compared with high performance liquid chromatography (HPLC) analysis.

Results: The percentage error between TLC and HPLC analyses was ranged from (-) 5.18 to (+) 5.51. Bacterial strains Arthrobacter simplex IAM 1660, Nocardia sp. MTCC 1534, Pseudomonas putida MTCC 1194 and Rhodococcus rhodochorus MTCC 291 respectively yielded 79.7 (72 h), 63.9 (72 h), 69.8 (96 h) and 83.2 (96 h) mole percent bioconversion of 6-methylene androstenedione to exemestane.

Conclusion: Rhodococcus rhodochorus MTCC 291 was found to be the most suitable organism for the bioconversion and may be used to develop an eco-friendly route to replace chemical synthesis that eliminates the use of toxic chemicals and side products.


Keywords


TLC image analysis, 6-Methylene androstenedione, C-1(2)-Dehydrogenation, Exemestane, Rhodococcus rhodochorus MTCC 291

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About this article

Title

QUANTIFICATION OF EXEMESTANE ACCUMULATION DURING MICROBIAL BIOCONVERSION BY TLC IMAGE ANALYSIS

Keywords

TLC image analysis, 6-Methylene androstenedione, C-1(2)-Dehydrogenation, Exemestane, Rhodococcus rhodochorus MTCC 291

DOI

10.22159/ijpps.2017v9i8.19201

Date

01-08-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 8, 2017 Page: 105-109

Online ISSN

0975-1491

Statistics

19 Views | 89 Downloads

Authors & Affiliations

Prachi Patil
School of Pharmacy, Devi Ahilya University, Indore, India
India

Rajesh Sharma
School of Pharmacy, Devi Ahilya University, Indore, India
India

Tushar Banerjee
School of Life Sciences, Devi Ahilya University, Indore-452001, India
India

Shridhar Patil
School of Life Sciences, Devi Ahilya University, Indore 452001, India
India


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